Phototropism and Pigment Production in Sordaria in Relation to Quality of Light

Ingold, C.T.; Hadland, Susan A.

doi:10.1093/aob/23.3.425

Cited by 10 publications

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“…Recently, the polar outgrowth and branching of young hyphae that emerge from the germination vesicles of ascospores were reported in Sordaria macrospora (Teichert 2017 ). Although S. fimicola has long been used for various aspects of fungal research (Ingold and Dring 1957 ; Ingold and Hadland 1959 ; Kitani and Whitehou 1974 ; Olive 1956 ), no strain generated via a genetic transformation method has yet been established for this organism to date. To expand the capabilities of S. fimicola in genetic analyses, in this study, we conducted the first successful transformation of S. fimicola and generated a histone H2B-mCherry-labeled strain, which was used to examine the nuclear dynamics during ascospore germination, sexual differentiation, and hyphal extension.…”

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Nuclear dynamics in the homothallic ascomycete Sordaria fimicola

Krobanan

Shen

2018

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BackgroundThe coprophilous ascomycete Sordaria fimicola usually reproduces sexually. Sexual differentiation in S. fimicola is accompanied by cellular and morphological changes, followed by multicellular tissue development to complete the sexual cycle. Although the morphological features of the sexual reproductive structure in S. fimicola have been well characterized, little is known about the nuclear dynamics and organization during these processes. Therefore, in this study, we successfully developed an Agrobacterium-mediated protoplast transformation protocol and generated histone H2B-mCherry-labeled S. fimicola strains. The life cycle of S. fimicola begins with germination of the ascospore and ends with the formation and discharge of new ascospores from the mature black sexual fruiting bodies, the so-called perithecia. The nuclear dynamics of the fluorescently labeled strains were examined during ascospore germination, hyphal elongation, and hyphal fusion using fluorescent microscopy.ResultsLive imaging revealed that the nuclei in the germlings and fusion hyphae during the pre-contact interaction are located adjacent to the tip.ConclusionsThis is the first report of the application of a fluorescence labeling technique in S. fimicola. This application will help researchers gain a better understanding of nuclear distribution and investigate the protein–protein interaction networks during fruiting body formation for advanced molecular genetic studies in S. fimicola.Electronic supplementary materialThe online version of this article (10.1186/s40529-018-0233-y) contains supplementary material, which is available to authorized users.

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