Photothermal effect for localized desorption of primary lymphocytes arrayed on an antibody/DNA-based biochip

Leroy, Loïc; Bombera, Radoslaw; Engel, Élodie; Calemczuk, R.; Laplatine, Loïc; Baganizi, Dieudonné R.; Marche, Patrice N.; Roupioz, Yoann; Livache, Thierry

doi:10.1039/c4lc00336e

Cited by 12 publications

(12 citation statements)

References 31 publications

(31 reference statements)

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“…Additionally, a sensor chip was assembled in a microfluidic device, which was integrated with SPRi. Targeted release was possible due to the laser-induced heating [ 170 ]. Piliarik et al has described the SPR immunosensor exploiting site-selective DNA-directed immobilization of antibodies against human chorionic gonadotropin (hCG) and activated leukocyte cell adhesion molecule (ALCAM), as biomarkers abundant in blood plasma [ 171 ].…”

Section: Affinity-based Oriented Ab Immobilization Strategiesmentioning

confidence: 99%

Recent Advancements in Receptor Layer Engineering for Applications in SPR-Based Immunodiagnostics

Drozd

Karoń

Malinowska

2021

Sensors

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The rapid progress in the development of surface plasmon resonance-based immunosensing platforms offers wide application possibilities in medical diagnostics as a label-free alternative to enzyme immunoassays. The early diagnosis of diseases or metabolic changes through the detection of biomarkers in body fluids requires methods characterized by a very good sensitivity and selectivity. In the case of the SPR technique, as well as other surface-sensitive detection strategies, the quality of the transducer-immunoreceptor interphase is crucial for maintaining the analytical reliability of an assay. In this work, an overview of general approaches to the design of functional SPR-immunoassays is presented. It covers both immunosensors, the design of which utilizes well-known and often commercially available substrates, as well as the latest solutions developed in-house. Various approaches employing chemical and passive binding, affinity-based antibody immobilization, and the introduction of nanomaterial-based surfaces are discussed. The essence of their influence on the improvement of the main analytical parameters of a given immunosensor is explained. Particular attention is paid to solutions compatible with the latest trends in the development of label-free immunosensors, such as platforms dedicated to real-time monitoring in a quasi-continuous mode, the use of in situ-generated receptor layers (elimination of the regeneration step), and biosensors using recombinant and labelled protein receptors.

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Section: Affinity-based Oriented Ab Immobilization Strategiesmentioning

confidence: 99%

Recent Advancements in Receptor Layer Engineering for Applications in SPR-Based Immunodiagnostics

Drozd

Karoń

Malinowska

2021

Sensors

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“…Moreover, for thicknesses of metal much larger than L z,metal the electric field of the SP is almost null at the glass/metal interface, so SP cannot decay into a photon in the glass either. They finally dissipate their energy into heat in the metal layer [41,42]. As a consequence, when an incident ray of light couples to SP, the intensity of the reflected ray decreases due to absorption.…”

Section: Optical Excitation Of Surface Plasmonsmentioning

confidence: 99%

“…For bacterial detection, image analyses can be extended to higher levels of complexity, such as the 2D dispersion of intensity (instead of the 1D histogram), the analysis of the noise frequency, or even a particle counting detection, thereby directly quantifying the number of bacteria and micro-colonies. Since the resolution limit when imaging is also the resolution limit when focusing a light beam, resolution optimized prisms could be used to perform individual cell sorting by inducing a very localized photo-thermal effect [42].…”

Section: Applicationsmentioning

confidence: 99%

Spatial resolution in prism-based surface plasmon resonance microscopy

Laplatine¹,

Leroy²,

Calemczuk³

et al. 2014

Opt. Express

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Several optical surface sensing techniques, such as Surface Plasmon Resonance (SPR), work by imaging the base of a prism by one of its faces. However, such a fundamental optical concern has not been fully analyzed and understood so far, and spatial resolution remains a critical and controversial issue. In SPR, the propagation length L(x) of the surface plasmon waves has been considered as the limiting factor. Here, we demonstrate that for unoptimized systems geometrical aberrations caused by the prism can be more limiting than the propagation length. By combining line-scan imaging mode with optimized prisms, we access the ultimate lateral resolution which is diffraction-limited by the object light diffusion. We describe several optimized configurations in water and discuss the trade-off between L(x) and sensitivity. The improvement of resolution is confirmed by imaging micro-structured PDMS stamps and individual living eukaryote cells and bacteria on field-of-view from 0.1 to 20 mm(2).

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“…Dans les dispositifs existant, le relargage est contrôlé mécaniquement [8], électriquement [9,10], thermiquement [11] ou par des enzymes [12,13]. Notre équipe a quant à elle développé un système de tri cellulaire sans marquage basé sur les propriétés thermodynamiques de l'ADN et sur la résonance des plasmons de surface (SPR) [14].…”

Section: Introductionunclassified

Chauffage par effet plasmon pour la désorption localisée de cellules capturées sur une biopuce

Engel¹,

Bombera²,

Leroy³

et al. 2016

isi

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Photo-thermal Effect for Localized Desorption of Primary Lymphocytes Arrayed on an Antibody/DNA-based Biochip)This work proposes a miniaturized system able to perform multiple cell capture followed by cell-type selective release from a biochip surface. Unlabeled lymphocytes are first specifically captured onto a DNA array by antibody-DNA conjugates. The immobilized cells are subsequently released under spatiotemporal control within local heating generated by intense Surface Plasmon Resonance (SPR) produced by laser illumination.

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Photothermal effect for localized desorption of primary lymphocytes arrayed on an antibody/DNA-based biochip

Cited by 12 publications

References 31 publications

Recent Advancements in Receptor Layer Engineering for Applications in SPR-Based Immunodiagnostics

Recent Advancements in Receptor Layer Engineering for Applications in SPR-Based Immunodiagnostics

Spatial resolution in prism-based surface plasmon resonance microscopy

Chauffage par effet plasmon pour la désorption localisée de cellules capturées sur une biopuce

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