Photothermal and optoacoustic spectroscopy: state of the art and prospects

Проскурнин, М. А.; Khabibullin, Vladislav R.; Usoltseva, Lilia O.; Vyrko, E.A.; Mikheev, Ivan V.; Volkov, Dmitrii S.

doi:10.3367/ufne.2021.05.038976

Cited by 18 publications

(19 citation statements)

References 585 publications

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“…The choice of operating parameters of the built setups is based on an analysis of the literature over the past decades and, in general, considers the main parameters used for dual-beam thermal-lens spectrometry (examples of mainly used thermal-lens spectrometers are given in the Supplementary Materials, Table S2 ). These are most commonly used configurations in thermal-lens measurements in applied chemistry and thermophysical measurements [ 3 , 4 , 5 , 6 , 61 , 65 ]. The spectrometers are built in three configurations ( Figure 1 and Figure 2 ).…”

Section: Resultsmentioning

confidence: 99%

“…In TLS, the refractive index gradient that arises when a sample is irradiated by an excitation beam acts as a lens-like element called the thermal lens [ 3 , 4 , 5 , 6 ]. If the sample has a negative refractive index temperature coefficient (

), then the thermal lens expands the transmitted beam.…”

Section: Methodsmentioning

confidence: 99%

“…Photothermal spectroscopy (PTS) solves a broad range of problems in the analysis and characterization of complex and heterogeneous systems based on the assessment of their thermal and optical properties [ 1 , 2 , 3 ]. Thermal-lens spectrometry (TLS) occupies an essential position in the group of photothermal methods and is used to solve many physicochemical problems [ 3 , 4 , 5 , 6 ]. TLS is used for monitoring the course of chemical reactions [ 7 , 8 ] and is widespread in biochemical analysis [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

“…Currently, most TLS applications rely on the use of the dual-beam (pump–probe) configuration [ 3 , 4 , 5 , 27 ]. As a rule, a probe beam is a continuous He–Ne laser.…”

Section: Introductionmentioning

confidence: 99%

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Accuracy of Measurements of Thermophysical Parameters by Dual-Beam Thermal-Lens Spectrometry

2023

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Thermal-lens spectrometry is a sensitive technique for determination of physicochemical properties and thermophysical parameters of various materials including heterogeneous systems and nanoparticles. In this paper, we consider the issues of the correctness (trueness) of measurements of the characteristic time of the thermal-lens effect and, thus, of the thermal diffusivity determined by dual-beam mode-mismatching thermal lensing. As sources of systematic errors, major factors—radiation sources, sample-cell and detector parameters, and general measurement parameters—are considered using several configurations of the thermal-lens setups, and their contributions are quantified or estimated. Furthermore, with aqueous ferroin and Sudan I in ethanol as inert colorants, the effects of the intermolecular distance of the absorbing substance on the correctness of finding the thermophysical parameters are considered. The recommendations for checking the operation of the thermal-lens setup to ensure the maximum accuracy are given. The results obtained help reducing the impact of each investigated factor on the value of systematic error and correctly measure the thermophysical parameters using thermal-lens spectrometry.

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Section: Resultsmentioning

confidence: 99%

), then the thermal lens expands the transmitted beam.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Currently, most TLS applications rely on the use of the dual-beam (pump–probe) configuration [ 3 , 4 , 5 , 27 ]. As a rule, a probe beam is a continuous He–Ne laser.…”

Section: Introductionmentioning

confidence: 99%

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Accuracy of Measurements of Thermophysical Parameters by Dual-Beam Thermal-Lens Spectrometry

2023

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“…Thus, TLS allows detection of the formation of Cyt c –CL directly in the solution at a low concentration of cytochrome c and lipid (10 μM and below). In addition, thermal lens measurements are significantly less dependent on light scattering than conventional spectrophotometry and depend on the heat transfer properties [ 24 , 27 ], which is vital for such light-scattering samples as suspensions of Cyt c –CL hydrophobic nanospheres. This is especially important at neutral pHs, where strong scattering of the formed complexes of cytochrome c with cardiolipin interferes with the registration of absorption spectra [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Composition of the Cytochrome c Complex with Cardiolipin by Thermal Lens Spectrometry

et al. 2023

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Thermal lens spectrometry along with spectrophotometric titration were used to assess the composition of the complex of oxidized cytochrome c (ferricytochrome c) with 1,1′,2,2′-tetraoleyl cardiolipin, which plays a key role in the initiation of apoptosis. Spectrophotometric titration was carried out for micromolar concentrations at which the complex is mainly insoluble, to assess the residual concentration in the solution and to estimate the solubility of the complex. Thermal lens spectrometry was used as a method of molecular absorption spectroscopy, which has two advantages over conventional optical transmission spectroscopy: the higher sensitivity of absorbance measurements and the possibility of studying the light absorption by chromophores and heat transfer in complex systems, such as living cells or tissues. Thermal lens measurements were carried out at nanomolar concentrations, where the complex is mainly in solution, i.e., under the conditions of its direct measurements. From the thermal lens measurements, the ratios of cytochrome c and cardiolipin in the complex were 50 at pH 7.4; 30 at pH 6.8; and 10 at pH 5.5, which fit well to the spectrophotometric data. The molecular solubility of the complex at pH 6.8–7.4 was estimated as 30 µmol/L.

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Time-resolved photoacoustics of channelrhodopsins: early energetics and light-driven volume changes

Walter

Schubert

Heberle

et al. 2022

Photochem Photobiol Sci

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In biological photoreceptors, the energy stored in early transient species is a key feature to drive the photocycle or a chain of reactions. Time-resolved photoacoustics (PA) can explore the energy landscape of transient species formed within few ns after photoexcitation, as well as volumetric changes (ΔV) of these intermediates with respect to the parental state. In this work, PA identified these important parameters for several channelrhodopsins, namely CaChR1 from Chlamydomonas augustae and CrChR2 from Chlamydomonas reinhardtii and various variants. PA has access to the sub-ns formation of the early photoproduct P1 and to its relaxation, provided that this latter process occurs within a few μs. We found that ΔVP1 for CaChR1 is ca. 12 mL/mol, while it is much smaller for CrChR2 (4.7 mL/mol) and for H. salinarum bacteriorhodopsin (HsBR, ΔVK = 2.8 mL/mol). PA experiments on variants strongly indicate that part of this large ΔVP1 value for CaChR1 is caused by the protonation dynamics of the Schiff base counterion complex involving E169 and D299. PA data further show that the energy level of P1 is higher in CrChR2 (ca. 96 kJ/mol) than in CaChr1 (ca. 46 kJ/mol), comparable to the energy level of the K state of HsBR (60 kJ/mol). Instrumental to gain these molecular values from the raw PA data was the estimation of the quantum yield (Φ) for P1 formation via transient spectroscopy; for both channelrhodopsins, ΦP2 was evaluated as ca. 0.4. Graphical Abstract

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Photothermal and optoacoustic spectroscopy: state of the art and prospects

Cited by 18 publications

References 585 publications

Accuracy of Measurements of Thermophysical Parameters by Dual-Beam Thermal-Lens Spectrometry

Accuracy of Measurements of Thermophysical Parameters by Dual-Beam Thermal-Lens Spectrometry

Composition of the Cytochrome c Complex with Cardiolipin by Thermal Lens Spectrometry

Time-resolved photoacoustics of channelrhodopsins: early energetics and light-driven volume changes

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