Photosystem II-Based Biosensors for the Detection of Photosynthetic Herbicides

Giardi, Maria Teresa; Pace, Emanuela

doi:10.1007/978-0-387-36672-2_13

Cited by 6 publications

(5 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It catalyzes the light-induced transfer of electrons from water to plastoquinone in a process that evolved oxygen. The activity of PS II can be inhibited by several groups of herbicides and heavy metals [66].…”

Section: Photosynthesis-based Biosensorsmentioning

confidence: 99%

Biosensors for Pesticide Detection: New Trends

Sassolas¹,

Prieto‐Simón²,

Marty³

2012

AJAC

185

View full text Add to dashboard Cite

Due to the large amounts of pesticides commonly used and their impact on health, prompt and accurate pesticide analysis is important. This review gives an overview of recent advances and new trends in biosensors for pesticide detection. Optical, electrochemical and piezoelectric biosensors have been reported based on the detection method. In this review biosensors have been classified according to the immobilized biorecognition element: enzymes, cells, antibodies and, more rarely, DNA. The use of tailor-designed biomolecules, such as aptamers and molecularly imprinted polymers, is reviewed. Artificial Neural Networks, that allow the analysis of pesticide mixtures are also presented. Recent advances in the field of nanomaterials merit special mention. The incorporation of nanomaterials provides highly sensitive sensing devices allowing the efficient detection of pesticides

show abstract

Section: Photosynthesis-based Biosensorsmentioning

confidence: 99%

Biosensors for Pesticide Detection: New Trends

Sassolas¹,

Prieto‐Simón²,

Marty³

2012

AJAC

185

View full text Add to dashboard Cite

show abstract

“…In simplest formation, they are composed of biological recognition elements (BREs) and signal probes. 4 Identifying BREs with strong binding affinity and specificity to target biomarkers is critical for biosensor performance. 5 Currently, these limitations restrict the broad use of biosensors with real samples.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Bacterial biosensors are emerging tools for rapid diagnostics and detection of pathogens and their drug-resistant variants. In simplest formation, they are composed of biological recognition elements (BREs) and signal probes . Identifying BREs with strong binding affinity and specificity to target biomarkers is critical for biosensor performance .…”

Section: Introductionmentioning

confidence: 99%

PepDRED: De Novo Peptide Design with Strong Binding Affinity for Target Protein

Azmat,

Ghalandari,

Jessica

et al. 2023

Anal. Chem.

View full text Add to dashboard Cite

De novo design of peptides that bind specifically to functional proteins is beneficial for diagnostics and therapeutics. However, complex permutations and combinations of amino acids pose significant challenges to the rational design of peptides with desirable stability and affinity. Herein, we develop a computational-based evolution method, namely, peptidomimetics-driven recognition elements design (PepDRED), to derive hemoglobin-inspired peptidomimetics. PepDRED mimics the natural evolutionism pipeline to generate stable apovariant (AVs) structures for wild-type counterparts via automated point mutations and validates their efficiency through free binding energy analysis and per residue energy decomposition analysis. For application demonstration, we applied PepDRED to design de novo peptides to bind FhuA, a typical TonB-dependent transporter (TBDT). TBDTs are Gram-negative bacterial outer membrane proteins responsible for iron transport and vital for bacterial resistance. PepDRED generated a pool of AVs and proceeded to reach an optimized peptide, AV440, with a remarkable binding affinity of −21 kcal/mol. AV440 is ∼2.5-fold stronger than the existing FhuA inhibitor Microcin J25. Network energy analysis further unveils that incorporating methionine (M42) in the N-terminal region significantly enhances inter-residue contacts and binding affinity. PepDRED offers a prompt and efficient in silico approach to develop potent peptide candidates for target proteins.

show abstract

“…Such interest could be explained by a fascination with this complex process and also by the possibility of applying the inner photosynthetic mechanisms in the most diversified fields. This last topic is really interesting, and examples of applications of photosynthetic materials can be found in the environmental or in the energy transfer fields [1][2][3][4][5]. Photosynthetic materials, such as the chloroplasts, thylakoid membranes, Photosystem II (PSII) from higher plants and the bacterial reaction centers (RC) could provide a great opportunity to built photo-optical electronic devices based on chloroplast proteins.…”

Section: Introductionmentioning

confidence: 99%

“…These pollutants are present in the industrial waste effluents and agricultural run-off and pose a risk of contamination of agricultural soils, surface and ground waters and a consequent accumulation in plant and animal tissues. Environmental monitoring is of fundamental importance for preservation of the planet and of its natural resources, and this is why the techniques and the procedures for detecting ecosystem pollutants are always in demand [1]. An interesting way for exploiting photosynthetic samples in the environmental field consists in performing bioassays in solutions: the observation of changes in the chemical-physical properties of the materials under test, in the presence of pollutant substances, can give useful information about mechanisms of interaction between photosynthetic systems and toxic compounds.…”

Section: Introductionmentioning

confidence: 99%

Interactions between heavy metals and photosynthetic materials studied by optical techniques

Ventrella

Catucci

Piletska

et al. 2009

Bioelectrochemistry

View full text Add to dashboard Cite

In this work studies on rapid inhibitory interactions between heavy metals and photosynthetic materials at different organization levels were carried out by optical assay techniques, investigating the possibility of applications in the heavy metal detection field. Spinach chloroplasts, thylakoids and Photosystem II proteins were employed as biotools in combination with colorimetric assays based on dichlorophenol indophenole (DCIP) photoreduction and on fluorescence emission techniques. It was found that copper and mercury demonstrated a strong and rapid photosynthetic activity inhibition, that varied from proteins to membranes, while other metals like nickel, cobalt and manganese produced only slight inhibition effects on all tested photosynthetic materials. By emission measurements, only copper was found to rapidly influence the photosynthetic material signals. These findings give interesting information about the rapid effects of heavy metals on isolated photosynthetic samples, and are in addition to the literature data concerning the effects of growth in heavy metal enriched media.

show abstract

Photosystem II-Based Biosensors for the Detection of Photosynthetic Herbicides

Cited by 6 publications

References 54 publications

Biosensors for Pesticide Detection: New Trends

Biosensors for Pesticide Detection: New Trends

PepDRED: De Novo Peptide Design with Strong Binding Affinity for Target Protein

Interactions between heavy metals and photosynthetic materials studied by optical techniques

Contact Info

Product

Resources

About