Photosynthetic characterization of flavodoxin-expressing tobacco plants reveals a high light acclimation-like phenotype

Gómez, Rodrigo; Figueroa, Nicolás; Melzer, Michael; Hajirezaei, Mohammad‐Reza; Carrillo, Néstor; Lodeyro, Anabella F.

doi:10.1016/j.bbabio.2020.148211

Cited by 15 publications

(16 citation statements)

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“…In plants, ROS formation are in the electron transport chains (ETC) of the chloroplasts and the mitochondria. At low levels, ROS are key factors in physiological redox signaling when plants response to stresses, while on the contrary, they are associated with oxidative stress (Gómez et al 2020). In photosystem I (PSI), the electron transport chain light energy is drived electrons to the acceptor molecule.…”

Section: Discussionmentioning

confidence: 99%

Cys-SH based Quantitative Redox Proteomics of Salt Induced Response in Sugar Beet Monosomic Addition Line M14

Zhang

et al. 2021

Preprint

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Background: Salt stress is a major abiotic stress that limits plant growth, development and productivity. Studying the molecular mechanisms of salt stress tolerance may help to enhance crop productivity. Sugar beet monosomic addition line M14 exhibits tolerance to salt stress. Results: In this work, the changes in the BvM14 proteome and redox proteome induced by salt stress were analyzed using a multiplex iodoTMTRAQ double labeling quantitative proteomics approach. A total of 80 proteins were differentially expressed under salt stress. Interestingly, 42 potential redox-regulated proteins showed differential redox change under salt stress. A large proportion of the redox proteins were involved in photosynthesis, ROS homeostasis and other pathways. For example, ribulose bisphosphate carboxylase/oxygenase activase changed in its redox state after salt treatments. In addition, three redox proteins involved in regulation of ROS homeostasis were also changed in redox states. Transcription levels of eighteen differential proteins and redox proteins were profiled. Conclusions: The results showed involvement of protein redox modifications in BvM14 salt stress response and revealed the short-term salt responsive mechanisms. The knowledge may inform marker-based breeding effort of sugar beet and other crops for stress resilience and high yield.

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Section: Discussionmentioning

confidence: 99%

Cys-SH based Quantitative Redox Proteomics of Salt Induced Response in Sugar Beet Monosomic Addition Line M14

Zhang

et al. 2021

Preprint

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“…Indeed, the flavoprotein is typically induced as an adaptive resource under environmental hardships that compromise Fd expression and activity, including osmotic stress, high light, heat, oxidants and especially, iron starvation (reviewed in [ 17 , 39 ]). Moreover, the introduction of Fld in plants via genetic engineering increased tolerance to multiple stresses [ 15 , 22 , 40 ] and complemented Fd-deficient plants [ 41 ]. While this protective effect is clearly associated to Fd substitution, Fld affects plant development [ 22 , 42 ], and transcriptional profiles ( Figure 3 , [ 31 ]) in the absence of stress, when Fd levels are not down-regulated.…”

Section: Discussionmentioning

confidence: 99%

“…While this protective effect is clearly associated to Fd substitution, Fld affects plant development [ 22 , 42 ], and transcriptional profiles ( Figure 3 , [ 31 ]) in the absence of stress, when Fd levels are not down-regulated. The functional basis for these effects remain yet to be elucidated, but they depend on the relative proportions of the two electron carriers [ 40 ], suggesting that they interact with common partners in the redox network of the chloroplast, and that the magnitude of the Fld impact might vary between species accumulating different Fd amounts. Analysis of plastoquinone (PQ) redox status revealed that Fld prevented over-reduction of the PETC [ 40 ], indicating that the flavoprotein was able to function as electron sink in chloroplasts despite eons of evolutionary divergence between cyanobacteria and terrestrial plants [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

“…The functional basis for these effects remain yet to be elucidated, but they depend on the relative proportions of the two electron carriers [ 40 ], suggesting that they interact with common partners in the redox network of the chloroplast, and that the magnitude of the Fld impact might vary between species accumulating different Fd amounts. Analysis of plastoquinone (PQ) redox status revealed that Fld prevented over-reduction of the PETC [ 40 ], indicating that the flavoprotein was able to function as electron sink in chloroplasts despite eons of evolutionary divergence between cyanobacteria and terrestrial plants [ 18 ]. These promising results encouraged application of the Fld approach to crops and to the most agronomically relevant stress, drought.…”

Section: Discussionmentioning

confidence: 99%

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Transcriptional and Metabolic Profiling of Potato Plants Expressing a Plastid-Targeted Electron Shuttle Reveal Modulation of Genes Associated to Drought Tolerance by Chloroplast Redox Poise

Karlusich

Arce

Shahinnia

et al. 2020

IJMS

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Water limitation represents the main environmental constraint affecting crop yield worldwide. Photosynthesis is a primary drought target, resulting in over-reduction of the photosynthetic electron transport chain and increased production of reactive oxygen species in plastids. Manipulation of chloroplast electron distribution by introducing alternative electron transport sinks has been shown to increase plant tolerance to multiple environmental challenges including hydric stress, suggesting that a similar strategy could be used to improve drought tolerance in crops. We show herein that the expression of the cyanobacterial electron shuttle flavodoxin in potato chloroplasts protected photosynthetic activities even at a pre-symptomatic stage of drought. Transcriptional and metabolic profiling revealed an attenuated response to the adverse condition in flavodoxin-expressing plants, correlating with their increased stress tolerance. Interestingly, 5–6% of leaf-expressed genes were affected by flavodoxin in the absence of drought, representing pathways modulated by chloroplast redox status during normal growth. About 300 of these genes potentially contribute to stress acclimation as their modulation by flavodoxin proceeds in the same direction as their drought response in wild-type plants. Tuber yield losses under chronic water limitation were mitigated in flavodoxin-expressing plants, indicating that the flavoprotein has the potential to improve major agronomic traits in potato.

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“…Based on the number of isoprene unit, terpenoids are classified into monoterpene, sesquiterpene, diterpene, sesterterpene, triterpene, and tetraterpene. Increasing studies have shown that terpenoids play essential roles in plant respiration, photosynthesis, and growth and development (Daviere and Achard 2013;Gomez et al 2020;Phillips et al 2008;Umehara et al 2008;Wanke et al 2001). They also mediate several other biological processes, such as resistance to various biotic and abiotic stresses, volatile emission for attracting pollinators in reproductive processes, and intracellular signal transduction (Block et al 2019;Hanhineva et al 2008;Hemmerlin et al 2003;Hunter 2007;Ramya et al 2020;Sacchettini and Poulter 1997).…”

Section: Introductionmentioning

confidence: 99%

A systematic review of 1-Deoxy-D-xylulose-5-phosphate synthase in terpenoid biosynthesis in plants

et al. 2021

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1-Deoxy-D-xylulose-5-phosphate synthase (DXS) is the first rate-limiting enzyme involved in the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for terpenoids biosynthesis. Up to date, three types of DXS (DXS1, DXS2, and DXS3) have been identified in plants. In this review, we searched all the plant DXS orthologs currently registered in the NCBI database, and selected 40 of them for phylogenetic, physicochemical properties, and structure analyses. Our bioinformatic analysis indicates that three types of DXS are all stable and conservative and that the evolutionary discrimination among them is very clear. We provide a summary of our current understanding about expression and regulation of DXS based on reports published in the past two decades, which demonstrates that DXS plays essential roles in many physiological processes, such as photosynthesis, phytohormone regulation, adversity resistance, and pathogen defense. However, additional studies are still needed for a better understanding of the molecular mechanisms underlying the roles of DXS in these biological processes. Moreover, clarifying the X-ray diffraction structure of DXS from plants remains a major issue to be addressed. This review will lay a foundation for further studies of DXS in plants.

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Photosynthetic characterization of flavodoxin-expressing tobacco plants reveals a high light acclimation-like phenotype

Cited by 15 publications

References 70 publications

Cys-SH based Quantitative Redox Proteomics of Salt Induced Response in Sugar Beet Monosomic Addition Line M14

Cys-SH based Quantitative Redox Proteomics of Salt Induced Response in Sugar Beet Monosomic Addition Line M14

Transcriptional and Metabolic Profiling of Potato Plants Expressing a Plastid-Targeted Electron Shuttle Reveal Modulation of Genes Associated to Drought Tolerance by Chloroplast Redox Poise

A systematic review of 1-Deoxy-D-xylulose-5-phosphate synthase in terpenoid biosynthesis in plants

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