Photosynthetic alterations of pea leaves infected systemically by pea enation mosaic virus: A coordinated decrease in efficiencies of CO2 assimilation and photosystem II photochemistry

Kyseláková, Helena; Prokopová, Jitka; Nauš, Jan; Novák, Ondřej; Navrátil, Milan; Šafářová, Dana; Špundová, Martina; Ilı́k, Petr

doi:10.1016/j.plaphy.2011.08.006

Cited by 45 publications

(41 citation statements)

References 55 publications

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“…Merkur plants during their compatible interaction with Pea enation mosaic virus (PEMV) (Kyseláková et al 2011). Since the stress caused by artificial PEMV infection includes also mechanical stress due to leaf abrasion with carborundum, we have used a mock inoculation treatment to study the combination of physiological responses.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, in the fresh material the POX activity and accumulation of H 2 O 2 and NO were localised histochemically. In order to ascertain the presence of the viruses in PEMV plants, the remaining leaves were analysed by enzyme-linked immunosorbent assay double antibody sandwich (ELISA-DAS) -for details see Kyseláková et al (2011).…”

Section: Plantmentioning

confidence: 99%

“…Similarly to the initiation of galls or cancer, the formation of enations is presumably linked to the alteration of phytohormone levels, especially cytokinins and auxins, which leads to the loss of cell cycle control (Choi et al 2011). Our previous work has been focused on the changes in pea photosynthesis during 40 days of PEMV pathogenesis (Kyseláková et al 2011). In this follow-up study we are interested namely in oxidative processes and phytohormone signalling, which might influence the systemic plant response and enation formation.…”

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confidence: 99%

“…Both enzymatic and non-enzymatic antioxidant systems are involved in maintaining the levels and relative proportions of individual RONS molecules. Radical intermediates formed during lipid peroxidation participate in the oxidation of pigment molecules and thus may be partially responsible for chlorosis (RiedleBauer 2000; Kyseláková et al 2011). Peroxidase (POX) not only scavenges H 2 O 2 , but it is also able to catalyse its formation and thus contributes to the oxidative stress during plant-virus interaction.…”

mentioning

confidence: 99%

“…Using both mock-inoculated (MI) and control (C) plants we were able to distinguish the effects of mechanical stress and natural senescence from the processes induced by PEMV infection. The importance of precise regulation of oxidative processes, as well as phytohormone and Hsp70 levels, for PEMV-induced tissue reorganisation are discussed together with the changes in photosynthetic parameters published earlier by Kyseláková et al (2011). nd true leaves of plants were inoculated by gently rubbing of approximately 1 ml of viral inoculum over the leaf surface, after the leaf had been gently wounded with carborundum and subsequently rinsed with sterile water.…”

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confidence: 99%

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Reactive oxygen and nitrogen species and hormone signalling in systemic infection of pea by Pea enation mosaic virus

Kyseláková¹,

Sedlářová²,

Kubala³

et al. 2013

Plant Prot. Sci.

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Kyseláková H., Sedlářová M., Kubala M., Nožková V., Piterková J., Luhová L., Novák O., Ilík P. (2013): Reactive oxygen and nitrogen species and hormone signalling in systemic infection of pea by Pea enation mosaic virus. Plant Protect. Sci., 49: 105-119.The physiological responses of pea plants during 40 days of compatible interaction with Pea enation mosaic virus (PEMV) were evaluated. PEMV induces systemic changes in the concentration of phytohormones. At 5 days post inoculation (dpi), a simultaneous increase in abscisic acid (ABA) level and a decrease in salicylic acid (SA) level was observed, which is known to be involved in the suppression of hypersensitive reaction. In our pathosystem it preceded the virus presence in the systemic leaves. PEMV induces the accumulation of ABA, SA and Hsp70 and enhances peroxidase (POX) activity at 15 dpi, when it is already transmitted throughout the plant. The formation of enations was preceded by a local accumulation of nitric oxide, which was followed by the appearance of reactive oxygen species, mostly in the vicinity of leaf veins. Such heterogeneity suggests involvement of these molecules in the control of hyperplasia and tissue differentiation.

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Section: Resultsmentioning

confidence: 99%

Section: Plantmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Reactive oxygen and nitrogen species and hormone signalling in systemic infection of pea by Pea enation mosaic virus

Kyseláková¹,

Sedlářová²,

Kubala³

et al. 2013

Plant Prot. Sci.

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Blue Native/SDS‐PAGE and iTRAQ‐Based Chloroplasts Proteomics Analysis of Nicotiana tabacum Leaves Infected with M Strain of Cucumber Mosaic Virus Reveals Several Proteins Involved in Chlorosis Symptoms

Lei

Kong

et al. 2017

Proteomics

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Virus infection in plants involves necrosis, chlorosis, and mosaic. The M strain of cucumber mosaic virus (M-CMV) has six distinct symptoms: vein clearing, mosaic, chlorosis, partial green recovery, complete green recovery, and secondary mosaic. Chlorosis indicates the loss of chlorophyll which is highly abundant in plant leaves and plays essential roles in photosynthesis. Blue native/SDS-PAGE combined with mass spectrum was performed to detect the location of virus, and proteomic analysis of chloroplast isolated from virus-infected plants was performed to quantify the changes of individual proteins in order to gain a global view of the total chloroplast protein dynamics during the virus infection. Among the 438 proteins quantified, 33 showed a more than twofold change in abundance, of which 22 are involved in the light-dependent reactions and five in the Calvin cycle. The dynamic change of these proteins indicates that light-dependent reactions are down-accumulated, and the Calvin cycle was up-accumulated during virus infection. In addition to the proteins involved in photosynthesis, tubulin was up-accumulated in virus-infected plant, which might contribute to the autophagic process during plant infection. In conclusion, this extensive proteomic investigation on intact chloroplasts of virus-infected tobacco leaves provided some important novel information on chlorosis mechanisms induced by virus infection.

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Photosynthetic Modulation in Response to Plant Activity and Environment

Adams

2018

Advances in Photosynthesis and Respiration

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Photosynthetic alterations of pea leaves infected systemically by pea enation mosaic virus: A coordinated decrease in efficiencies of CO2 assimilation and photosystem II photochemistry

Cited by 45 publications

References 55 publications

Reactive oxygen and nitrogen species and hormone signalling in systemic infection of pea by Pea enation mosaic virus

Reactive oxygen and nitrogen species and hormone signalling in systemic infection of pea by Pea enation mosaic virus

Blue Native/SDS‐PAGE and iTRAQ‐Based Chloroplasts Proteomics Analysis of Nicotiana tabacum Leaves Infected with M Strain of Cucumber Mosaic Virus Reveals Several Proteins Involved in Chlorosis Symptoms

Photosynthetic Modulation in Response to Plant Activity and Environment

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