Photosynthesis driven conversion of carbon dioxide to fatty alcohols and hydrocarbons in cyanobacteria

Tan, Xiaoming; Yao, Lun; Gao, Qianqian; Wang, Weihua; Qi, Fengxia; Lü, Xuefeng

doi:10.1016/j.ymben.2011.01.001

Cited by 197 publications

(134 citation statements)

References 30 publications

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“…The hydrocarbon composition of Synechocystis sp. PCC 6803 is consistent with the previous reports of Tan et al [29] and Guan et al [40]. However, these results contrast with a recent report that this strain produces 9-heptadecene [18].…”

Section: Discussionsupporting

confidence: 90%

“…Although the baeocystous type (subsection II) and the ramified type (subsection V) exhibit unicellular and filamentous phenotypes, respectively [6], further study of these subsections is necessary for a thorough understanding of the hydrocarbon distribution in cyanobacteria on the phylum level. Nostoc spongiaeforme FACHB 130, which showed a greater hydrocarbon production than all wild type strains previously reported [18,29], was identified as a prospective strain for the production of hydrocarbons with a biomass productivity of 1.3 g/L DCW and a hydrocarbon yield of 1.8 mg/g DCW ( Table 2). It is well known that hydrocarbons can constitute up to 75% of the DCW of Botryococcus braunii, an eukaryotic microalga belonging to the green algae division of chlorophyta [35].…”

Section: Discussionmentioning

confidence: 85%

“…They are known to be monophyletic but morphologically diverse, and traditionally, they are divided into five major subsections according to their engineering strategies. Our previous work demonstrated the viability of a cyanobacteria-based platform that targets fatty acidbased biofuels and discussed the possibility of controlling and regulating the properties of hydrocarbon fuels by choosing different starting cyanobacterial strains or by manipulating hydrocarbon biosynthesis pathways [2,29]. However, why cyanobacterial species produce hydrocarbons and how different types of hydrocarbons are synthesized are phenomena that have not yet been elucidated.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Hydrocarbon profiles and phylogenetic analyses of diversified cyanobacterial species

Liu¹,

Zhu²,

Lü³

et al. 2013

Applied Energy

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

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Hydrocarbon profiles and phylogenetic analyses of diversified cyanobacterial species

Liu¹,

Zhu²,

Lü³

et al. 2013

Applied Energy

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“…Considering these advantages, microalgae biofuels have been recognized as the only current renewable source of liquid transportation fuel which is compatible with the existing engines and distribution systems (Schenk et al 2008). Microalgae, including cyanobacteria, have been reported to generate a variety of lipids, hydrocarbons, fatty alcohols, and other complex oils (Metzger and Largeau 2005, Guschina and Harwood 2006, Schirmer et al 2010, Tan et al 2011. Although not all algal oils are suitable for biodiesel The flasks were incubated on an orbital shaker (Vision Scientific, Bucheon, Korea) at 160 rpm and 15°C under cool fluorescent lighting (approximately 70 μmol photons m -2 s -1 ), until growth was apparent.…”

Section: Introductionmentioning

confidence: 99%

Natural production of alkane by an easily harvested freshwater cyanobacterium, Phormidium autumnale KNUA026

Chang¹,

Hong²,

Chae³

et al. 2013

ALGAE

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A freshwater cyanobacterium, Phormidium autumnale KNUA026, was isolated from puddles of icy water in Gyeongsan City, South Korea and its potential as a biofuel feedstock was investigated. Maximal growth was obtained when the culture was incubated at 25°C and around pH 9.0. The total lipid content of the isolate was approximately 14.0% of dry weight and it was found that strain KNUA026 was able to autotrophically synthesize heptadecane (C 17 H 36 ) which can be directly used as fuel without requiring a transesterification step. As this benthic cyanobacterium was capable of forming thick mats, it could be easily harvested by gravitational settling and this property may reduce the cost of production in commercial applications. Hence, P. autumnale KNUA026 appears to be a promising resource for use in the production of microalgae-based biofuels.Key Words: algae-based fuel; alkane; cyanobacterium; heptadecane; Phormidium autumnale INTRODUCTIONIn recognition of the current energy crisis, alternative energy sources are currently being explored and developed at an increasing pace; biofuels in particular have received a considerable amount of attention over the past few years. Conventional biofuels, such as bioethanol and biodiesel, are produced primarily from cereal crops and oil seeds. However, the mass production of crop-based biofuels has resulted in serious side effects, such as increases in food prices, deforestation, and carbon emissions (Sims et al. 2010). Thus, algae-based biofuels have been recognized as an attractive option, as they use minimal land resources (Chisti 2007) and do not compete with food production (Huang et al. 2010). Additionally, algaebased biofuels have several other advantages, including rapid growth rates (Schenk et al. 2008), higher lipid contents (Hu et al. 2008), and higher CO 2 uptake rates (Jorquera et al. 2010) relative to other energy crops. Considering these advantages, microalgae biofuels have been recognized as the only current renewable source of liquid transportation fuel which is compatible with the existing engines and distribution systems (Schenk et al. 2008). Microalgae, including cyanobacteria, have been reported to generate a variety of lipids, hydrocarbons, fatty alcohols, and other complex oils ( ), until growth was apparent. An aliquot of the brown-colored biomass was taken by pipetting, and was sonicated for approximately 3-5 s using an ultrasonic cell disruptor (Model 550; Fisher Scientific, Pittsburgh, PA, USA). The filaments were then transferred onto BG-11 agar plates containing 100 µg mL -1 of meropenem (Yuhan Pharmaceuticals, Ochang, Korea) and were incubated in the dark for 24 h to eliminate contaminating bacteria (Choi et al. 2008). The culture was then inoculated onto fresh BG-11 agar plates and incubated for 14 days under a light : dark cycle (16 : 8 h) at 15°C. Morphological and molecular identificationThe isolate was grown in BG-11(+) medium for 21 days. Live cells were harvested by centrifugation at 3,000 ×g for 5 min, washed with sterile distilled water, ...

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“…PCC6803 [141,142]. Fatty alcohols such as hexadecanol and octadecanol can be produced by introducing fatty acyl-CoA reductase genes from jojoba, which catalyze a fatty-acyl-ACP (Figure 4) [143] (Table 1).…”

Section: Metabolic Modification For Various Biofuel and Biochemical Pmentioning

confidence: 99%

An overview on biofuel and biochemical production by photosynthetic microorganisms with understanding of the metabolism and by metabolic engineering together with efficient cultivation and downstream processing

Sarkar

Shimizu

2015

Bioresour. Bioprocess.

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Biofuel and biochemical production by photosynthetic microorganisms such as cyanobacteria and algae is attractive to improve energy security and to reduce CO 2 emission, contributing to the environmental problems such as global warming. Although biofuel production by photosynthetic microorganisms is called as the third generation biofuels, and significant innovation is necessary for the feasibility in practice, these fuels are attractive due to renewable and potentially carbon neutral resources. Moreover, photosynthetic microorganisms are attractive since they can grow on non-arable land and utilize saline and wastewater streams. Highly versatile and genetically tractable photosynthetic microorganisms need to capture solar energy and convert atmospheric and waste CO 2 to high-energy chemical products. Understanding of the metabolism and the efficient metabolic engineering of the photosynthetic organisms together with cultivation and separation processes as well as increased CO 2 assimilation enables the enhancement of the feasibility of biofuel and biochemical production.

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Photosynthesis driven conversion of carbon dioxide to fatty alcohols and hydrocarbons in cyanobacteria

Cited by 197 publications

References 30 publications

Hydrocarbon profiles and phylogenetic analyses of diversified cyanobacterial species

Hydrocarbon profiles and phylogenetic analyses of diversified cyanobacterial species

Natural production of alkane by an easily harvested freshwater cyanobacterium, Phormidium autumnale KNUA026

An overview on biofuel and biochemical production by photosynthetic microorganisms with understanding of the metabolism and by metabolic engineering together with efficient cultivation and downstream processing

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