Photosensitizer Radachlorin®: Skin cancer PDT phase II clinical trials

Kochneva, Elena V.; Филоненко, Е. В.; Vakulovskaya, Elena G.; Scherbakova, E G; Seliverstov, Oleg V.; Markichev, Nikolay A.; Решетников, А. В.

doi:10.1016/j.pdpdt.2010.07.006

Cited by 64 publications

(26 citation statements)

References 2 publications

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“…The microscopy images (Figure 9, S8) clearly showed that Hep-2 cells in groups without irradiation and in the negative control group and SiO 2 after irradiation had spindle shape, were in close contact with neighbouring cells and adhered to the surface of the culture flask. 46,47 We believe that the photo-induced cytotoxic effect of {Mo 6 I 8 } 0.001 @SiO 2 and {Mo 6 I 8 } 0.01 @SiO 2 is caused mostly by free particles rather than ones localised in endosomes, since they extend cytotoxic effect of ROS within the cytoplasm. The effect of concentration of the photosensitisers on the cells is demonstrated on Figure 10.…”

Section: Cellular Internalisation and Intracellular Distributionmentioning

confidence: 97%

Cellular internalisation, bioimaging and dark and photodynamic cytotoxicity of silica nanoparticles doped by {Mo₆I₈}⁴⁺ metal clusters

et al. 2016

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Silica nanoparticles (SNPs) doped by hexanuclear molybdenum cluster complexes [{Mo6X8}L6] n (X = Cl, Br, or I; L = various inorganic or organic ligands) have been recently suggested as materials with a high potential for biomedical applications due to both the outstanding photoluminescent properties and the ability to efficiently generate singlet oxygen upon photoirradiation. However, no studies were undertaken so far to prove this concept. Therefore, here we examined the potential of photoluminescent SNPs doped by {Mo6I8} 4+ for such applications as bioimaging and photodynamic therapy using human epidermoid larynx carcinoma (Hep-2) cell line as a model. Our results demonstrated both: (i) significant luminescence from cells with internalised molybdenum cluster-doped SNPs combined with the low cytotoxicity of particles in the darkness and (ii) significant cytotoxicity of the particles upon photoirradiation. Thus, this research provides strong experimental evidence for high potential of molybdenum-cluster-doped materials in such biomedical applications as optical bioimaging, biolabeling and photodynamic therapy.

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Section: Cellular Internalisation and Intracellular Distributionmentioning

confidence: 97%

Cellular internalisation, bioimaging and dark and photodynamic cytotoxicity of silica nanoparticles doped by {Mo₆I₈}⁴⁺ metal clusters

et al. 2016

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“…PDT is a minimally invasive therapeutic modality which has been shown to be effective against different types of malignancies like skin and esophageal cancer. 8,9 PDT has some advantage in cancer treatments, it has a less invasive than surgery and radiotherapy. It has no long-term side effects when used properly and PDT can be repeated many times at the same site if needed.…”

Section: Discussionmentioning

confidence: 99%

Effect of Photodynamic Therapy in Melanoma Skin Cancer Cell Line A375: in vivo Study

et al. 2014

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Background and ObjectivesAminolevulinic acid (ALA), a precursor of heme, which is converted to protoporphyrin IX, is used as an effective photosensitizer. The aim of this study is to evaluate the effect of photodynamic therapy (PDT) using 5-ALA in an in vivo experiment using melanoma skin cancer cell line A375. Materials and MethodsFemale nude mice (BALB/C/nu/nu), four weeks of age and 25-30 g (Narabio, Korea) in weight, were used in the study. When the actual tumor size reached more than 100-200 mm 3 , the mice were divided randomly into the PDT group (n = 7), LED (light emitting diode) group (n = 3), ALA group (n = 3), and control group (n = 7). ALA 375 mg/kg was used in the study as an intraperitoneally injected photosensitizer. The tumors were then irradiated using a 630 nm LED for 30 min after 3 hours in a dark room. Tumor dimensions were measured using a caliper at 0, 7, 14, 21, and 28 days after treatment in all groups and tumor volume was calculated. ResultsThere was no significant difference in the mean tumor volumes on 0 day before treatment in each group (p>0.05). However, significant differences in the mean tumor volumes were observed between the PDT and control groups at 7, 14, 21, and 28 days after treatment (p<0.05). ConclusionALA mediated PDT was found to be effective in melanoma skin cancer cell A375 induced tumor xenograft in nude mice.

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“…One and a half year after the treatment, CR was present in 92.8% patients who had CR as evaluated after 2 months [4].…”

Section: Our Phase II Clinical Studies Dating Back To 2003-2005 Demonmentioning

confidence: 97%

Melanoma: Surgery Subsequent to PDT—Is It Worth Doing? A 10-Year Pilot Trial Using Chlorin-Type Photosensitizer Bremachlorin<sup>&reg</sup>

Volkov¹,

Решетников²,

Vink³

2017

JCT

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Bremachlorin ® , also known as "Radachlorin Further studies described in the present paper were fulfilled in accordance with a skin melanoma PDT + surgery combined technique protocol in 2006-2016. Resulting from the present trial, up to the 100% complete responses were achieved after resection and plastic surgery following PDT, with no melanoma regrowth or metastases during 5 and 10-year observation periods. Conducting PDT one day prior to tumor dissection can guarantee to meet the ablastics principles at the subsequent surgery. At the same time, the PDT + surgery protocol can allow for dissecting the tumor with less distance from its edge, which seems to be important for the face localizations of tumors.

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Photosensitizer Radachlorin®: Skin cancer PDT phase II clinical trials

Cited by 64 publications

References 2 publications

Cellular internalisation, bioimaging and dark and photodynamic cytotoxicity of silica nanoparticles doped by {Mo₆I₈}⁴⁺ metal clusters

Cellular internalisation, bioimaging and dark and photodynamic cytotoxicity of silica nanoparticles doped by {Mo₆I₈}⁴⁺ metal clusters

Effect of Photodynamic Therapy in Melanoma Skin Cancer Cell Line A375: in vivo Study

Melanoma: Surgery Subsequent to PDT—Is It Worth Doing? A 10-Year Pilot Trial Using Chlorin-Type Photosensitizer Bremachlorin<sup>&reg</sup>

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Photosensitizer Radachlorin®: Skin cancer PDT phase II clinical trials

Cited by 64 publications

References 2 publications

Cellular internalisation, bioimaging and dark and photodynamic cytotoxicity of silica nanoparticles doped by {Mo6I8}4+ metal clusters

Cellular internalisation, bioimaging and dark and photodynamic cytotoxicity of silica nanoparticles doped by {Mo6I8}4+ metal clusters

Effect of Photodynamic Therapy in Melanoma Skin Cancer Cell Line A375: in vivo Study

Melanoma: Surgery Subsequent to PDT—Is It Worth Doing? A 10-Year Pilot Trial Using Chlorin-Type Photosensitizer Bremachlorin&lt;sup&gt;&amp;reg&lt;/sup&gt;

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Cellular internalisation, bioimaging and dark and photodynamic cytotoxicity of silica nanoparticles doped by {Mo₆I₈}⁴⁺ metal clusters

Cellular internalisation, bioimaging and dark and photodynamic cytotoxicity of silica nanoparticles doped by {Mo₆I₈}⁴⁺ metal clusters

Melanoma: Surgery Subsequent to PDT—Is It Worth Doing? A 10-Year Pilot Trial Using Chlorin-Type Photosensitizer Bremachlorin<sup>&reg</sup>