Effect of Photodynamic Therapy in Melanoma Skin Cancer Cell Line A375: in vivo Study

Abstract

Background and ObjectivesAminolevulinic acid (ALA), a precursor of heme, which is converted to protoporphyrin IX, is used as an effective photosensitizer. The aim of this study is to evaluate the effect of photodynamic therapy (PDT) using 5-ALA in an in vivo experiment using melanoma skin cancer cell line A375. Materials and MethodsFemale nude mice (BALB/C/nu/nu), four weeks of age and 25-30 g (Narabio, Korea) in weight, were used in the study. When the actual tumor size reached more than 100-200 mm 3 , the mice were divided randomly into the PDT group (n = 7), LED (light emitting diode) group (n = 3), ALA group (n = 3), and control group (n = 7). ALA 375 mg/kg was used in the study as an intraperitoneally injected photosensitizer. The tumors were then irradiated using a 630 nm LED for 30 min after 3 hours in a dark room. Tumor dimensions were measured using a caliper at 0, 7, 14, 21, and 28 days after treatment in all groups and tumor volume was calculated. ResultsThere was no significant difference in the mean tumor volumes on 0 day before treatment in each group (p>0.05). However, significant differences in the mean tumor volumes were observed between the PDT and control groups at 7, 14, 21, and 28 days after treatment (p<0.05). ConclusionALA mediated PDT was found to be effective in melanoma skin cancer cell A375 induced tumor xenograft in nude mice.

An Analysis of the Effects of In Vitro Photodynamic Therapy on Prostate Cancer Tissue by Histopathological Examination and Magnetic Resonance Imaging

“…This was an advanced squamous cell carcinoma obstructing the right main bronchus; PDT resulted in the opening of the bronchus. The second case was an early-stage squamous cell carcinoma of the right upper bronchus in March 1980 [ 50 , 51 ]. The variation in the initial tumor volumes (12–55 mm 3 ) between different tumors within the same treatment condition could introduce variations in local tumor control [ 52 ].…”

Synergistic Effects of Cisplatin-epigel and Interstitial KTP Laser Treatment on a Xenografted Squamous Cell Carcinoma

“…1,2 The KTP laser used in this study is the most frequently used laser in interstitial laser treatment because it penetrates approximately 5 to 6 mm in the tissue and is in the visible light region. [17][18][19] Cisplatin induces tissue necrosis when administered topically. Most drugs, particularly when administered directly to tumors with developed blood vessels, spread to the periphery of the tumor and throughout the body in a short period, increasing the concentration of anticancer drugs at the tumor site.…”

Reactive oxygen species overload: a review of plasma therapy and photobiomodulation for cancer treatment

“…(LLL) [18]. Different parameters of the lasers, including wavelength and output power, were shown to induce different reactions for different cell types, providing more opportunities for scientists to test specific parameters and freely explore various effects, diagnostic potential [88] and mechanisms aside from mere tissue ablation and vaporization [89,90].…”

“…Studies have noted the importance of taking advantage of exogenous and endogenous photosensitizers capable of absorbing radiation at the wavelength typically of ultraviolet A and near-visible light [89,91,92]. After radiation absorption, the photosensitizers transfer their energy to form singlet oxygens [93] which are expected to last for extended periods [94].…”