Photosensitization of in vitro biofilms formed on denture base resin

Freitas-Pontes, Karina M.; Gomes, Carlos Eduardo de Albuquerque; Carvalho, Bruna Marjorie Dias Frota de; Saboia, Rafael de Sousa Carvalho; Garcia, Bruna Albuquerque

doi:10.1016/j.prosdent.2014.01.001

Cited by 21 publications

(11 citation statements)

References 32 publications

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“…Thus, the present scheme of illumination would also be suitable for in vivo application. In previous studies on LED-based in vitro aPDT against P. aeruginosa, light intensities in the range of 100 -200 mW cm À2 were used (2,11,26). The intensity used in our study (45 mW cm À2 ) is lower than those values, and the lower intensity was possible due to the effects of the additives.…”

Section: Discussionmentioning

confidence: 94%

“…Methylene blue (MB), a cationic photosensitizer, has been widely used for aPDT because of its low cost and efficacy against a broad spectrum of both Gram-positive (+) and Gramnegative (À) bacteria. MB has been reported to be safe for use in humans (10,11). Cell death caused by aPDT depends on the probability of a dye adhering to the outside of the cell membrane and permeating across the cell membrane.…”

Section: Introductionmentioning

confidence: 99%

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Combined Addition of Ethanol and Ethylenediaminetetraacetic Acid Enhances Antibacterial and Antibiofilm Effects in Methylene Blue‐Mediated Photodynamic Treatment against Pseudomonas aeruginosa In Vitro

Sarker

Tsunoi

Haruyama

et al. 2020

Photochem & Photobiology

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Antimicrobial photodynamic treatment (aPDT) for infection with drug-resistant bacteria has received much attention. For P. aeruginosa, however, efficient formation of biofilms and the nature of Gram-negative bacteria often limit the efficacy of aPDT. In this study, we investigated the effects of ethanol and ethylenediaminetetraacetic acid (EDTA) as additives on bacterial viability, biofilm biomass, and structures of bacteria and biofilms in methylene blue (MB)-mediated aPDT in vitro. Matured P. aeruginosa biofilms were incubated with 32-µM MB solutions with different concentrations of additives and then illuminated with 665-nm light from an LED array. The combined addition of 10% ethanol and 10 mM EDTA to MB resulted in significantly greater bactericidal effects than those of MB alone and of MB with 10% ethanol or 10 mM EDTA. Crystal violet assays showed significant reductions in biofilm biomass by aPDT with addition of both ethanol and EDTA compared to that in the case of aPDT with MB alone. Scanning electron microscopy showed broken bacterial cells and reduction in the cell density and amount of biofilm under those conditions. Ethanol addition alone did not improve aPDT efficacy. Reduced amount of biofilm by EDTA addition would have improved the transportation of MB and ethanol to bacteria.

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Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Combined Addition of Ethanol and Ethylenediaminetetraacetic Acid Enhances Antibacterial and Antibiofilm Effects in Methylene Blue‐Mediated Photodynamic Treatment against Pseudomonas aeruginosa In Vitro

Sarker

Tsunoi

Haruyama

et al. 2020

Photochem & Photobiology

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“…Multiple bacterial biofilms have been shown to be eradicated using diode lasers (405–940 nm) and different photosensitizers on acrylic resin, glass, titanium, and zirconia, which in some cases was shown to be synergistic with antibiotic treatment [163, 167, 168, 169, 170, 171, 172, 173, 174]. LEDs (385–660 nm) have also been used to inhibit and eradicate bacterial formation on silicone coupons, dental implant surface, acrylic resin and titanium [162, 168, 175, 176, 177, 178, 179, 180]. A wide range of light in the electromagnetic spectrum has been shown to be useful in destroying bacteria, including the higher energy UVC and blue light, when combined with antibiotics [170].…”

Section: Main Textmentioning

confidence: 99%

Bacterial biofilm formation on implantable devices and approaches to its treatment and prevention

Khatoon

McTiernan

Suuronen

et al. 2018

Heliyon

869

652

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In living organisms, biofilms are defined as complex communities of bacteria residing within an exopolysaccharide matrix that adheres to a surface. In the clinic, they are typically the cause of chronic, nosocomial, and medical device-related infections. Due to the antibiotic-resistant nature of biofilms, the use of antibiotics alone is ineffective for treating biofilm-related infections. In this review, we present a brief overview of concepts of bacterial biofilm formation, and current state-of-the-art therapeutic approaches for preventing and treating biofilms. Also, we have reviewed the prevalence of such infections on medical devices and discussed the future challenges that need to be overcome in order to successfully treat biofilms using the novel technologies being developed.

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“…Several methods of disinfection have been suggested 3 , 5 , 28 , mainly including immersion of dentures in chemical solutions of sodium hypochlorite, alkaline glutaraldehyde, 4% chlorhexidine, chlorine dioxide, and denture cleansers such as alkaline peroxides, herbal and photodynamic therapy 1 , 11 , 13 , 15 . However, past studies have indicated that such solutions affect the physico-mechanical properties of the materials used to construct removable prostheses 19 , 25 .…”

Section: Introductionmentioning

confidence: 99%

Long-term microwaving of denture base materials: effects on dimensional, color and translucency stability

et al. 2018

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While the combined effect of microwave irradiation with cleansing solutions on denture base materials has been investigated, the effects of only using microwave irradiation and, more importantly, in a long-term basis, was not studied yet. Objective The purpose of this study was to evaluate the effect of a long-term repeated microwaving on the dimensional, color and translucency stability of acrylic and polyamide denture base materials.Material and Methods Thirty two specimens (32 mm x 10 mm x 2.5 mm) from polyamide (Valplast) and PMMA (Vertex Rapid Simplified) denture base materials were made. Eight specimens from each material were immersed in distilled water (control) and 8 were subjected to microwave exposure at 450 W for 3 minutes for a period simulating 224 days of daily disinfection. Linear dimension, color change (ΔE*) and translucency parameter (TP) were measured at baseline and after certain intervals up to 224 cycles of immersion, using a digital calliper and a portable colorimeter. The results were analysed using two-way repeated measures ANOVA to estimate possible differences among predetermined cycles and material type. Regression analysis was also performed to estimate the trend of changes with time. Statistical evaluations performed at a significance level of 5%.Results Data analysis showed significant changes in length at baseline with an increasing number of cycles (p<0.05) and a significant interaction of cycle-material (p<0.001). The ΔΕ* parameter was significantly higher with a higher number of cycles (p<0.001), but it did not vary between materials (p>0.05). TP decreased similarly in both materials following microwave action but in a significantly higher level for Valplast (p<0.001).Conclusions The results indicated that long-term repeated microwaving affects linear dimensional, color and translucency changes of both materials. Differences between PMMA and polyamide material were noted only in dimension and translucency changes.

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Photosensitization of in vitro biofilms formed on denture base resin

Abstract: Photodynamic therapy was effective in reducing E coli counts on biofilms formed on acrylic resin specimens. The inhibition of microorganism growth tended to be directly proportional to the amount of energy provided by the light-emitting diode.

Cited by 21 publications

References 32 publications

Combined Addition of Ethanol and Ethylenediaminetetraacetic Acid Enhances Antibacterial and Antibiofilm Effects in Methylene Blue‐Mediated Photodynamic Treatment against Pseudomonas aeruginosa In Vitro

Combined Addition of Ethanol and Ethylenediaminetetraacetic Acid Enhances Antibacterial and Antibiofilm Effects in Methylene Blue‐Mediated Photodynamic Treatment against Pseudomonas aeruginosa In Vitro

Bacterial biofilm formation on implantable devices and approaches to its treatment and prevention

Long-term microwaving of denture base materials: effects on dimensional, color and translucency stability

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