Photorezeptortransplantation in die degenerative Netzhaut

Borsch, Oliver; Santos-Ferreira, Tiago; Ader, Marius

doi:10.1055/s-0043-104421

Cited by 4 publications

(3 citation statements)

References 71 publications

(93 reference statements)

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“…Previously, we confirmed fetal and hPSC expression of GNGT2 as part of the human cone-enriched gene signature by RNAseq analysis [10] while others have shown early expression in the mouse retina [11]. Fluorescent reporter genes driven by various photoreceptor-specific promoters have demonstrated specific and robust labelling of target cells in rodents and have enabled numerous studies of retinal development, gene profiling, and transplantation [12][13][14][15][16][17]. To facilitate similar studies focused on cone photoreceptors, equally robust A c c e p t e d M a n u s c r i p t 7 tools created from hPSCs are required ideally labelling cells throughout development.…”

Section: Introductionmentioning

confidence: 57%

Generation of a Cone Photoreceptor-specific GNGT2 Reporter Line in Human Pluripotent Stem Cells

et al. 2022

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Fluorescent reporter lines generated in human pluripotent stem cells are a highly useful tool to track, isolate and analyse cell types and lineages in live cultures. Here, we generate the first human cone photoreceptor reporter cell line by CRISPR/Cas9 genome editing of a human embryonic stem cell (hESC) line to tag both alleles of the Guanine nucleotide-binding protein subunit gamma-T2 (GNGT2) gene with a mCherry reporter cassette. Three-dimensional optic vesicle-like structures were produced to verify reporter fidelity and track cones throughout their development in culture. The GNGT2-T2A-mCherry hESC line faithfully and robustly labels GNGT2-expressing cones throughout the entirety of their differentiation in vitro, recapitulating normal fetal expression of this gene. Our observations indicate that human cones undergo significant migratory activity during the course of differentiation in vitro. Consistent with this, our analysis of human fetal retinae from different stages of development finds positional differences of the cone population depending on their state of maturation. This novel reporter line will provide a useful tool for investigating human cone development and disease.

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Section: Introductionmentioning

confidence: 57%

Generation of a Cone Photoreceptor-specific GNGT2 Reporter Line in Human Pluripotent Stem Cells

et al. 2022

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“…Some studies have successfully established the cultivation of photoreceptor cells. While the technique of the sheet transplantation of allogenic photoreceptor cells in animals is successful, there are still doubts about the functional reconstruction [87][88][89][90]. Allogenic RPE transplantation can induce the immunological rejection reaction [91], but it is difficult to collect autologous RPE.…”

Section: Prevention and Intervention Strategies Of Rpmentioning

confidence: 99%

Cell Ferroptosis: New Mechanism and New Hope for Retinitis Pigmentosa

Yang

Lam

et al. 2021

Cells

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Retinitis pigmentosa (RP) is a leading cause of inherited retinal degeneration, with more than 60 gene mutations. Despite the genetic heterogenicity, photoreceptor cell damage remains the hallmark of RP pathology. As a result, RP patients usually suffer from reduced night vision, loss of peripheral vision, decreased visual acuity, and impaired color perception. Although photoreceptor cell death is the primary outcome of RP, the underlying mechanisms are not completely elucidated. Ferroptosis is a novel programmed cell death, with characteristic iron overload and lipid peroxidation. Recent studies, using in vitro and in vivo RP models, discovered the involvement of ferroptosis-associated cell death, suggesting a possible new mechanism for RP pathogenesis. In this review, we discuss the association between ferroptosis and photoreceptor cell damage, and its implication in the pathogenesis of RP. We propose that ferroptotic cell death not only opens up a new research area in RP, but may also serve as a novel therapeutic target for RP.

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“…Deshalb war zu beobachten, dass tatsächlich eine der Open Access publizierten Arbeiten [8] unter den meistzitierten Arbeiten für den IF 2017 war. Wenn man auf die Gesamtzitate schaut, sind es immerhin 4 Arbeiten, die unter den IF-relevanten Zitaten wieder auftauchen [5,8,9,15]. Man sieht damit, für die Nutzung eines Artikelssprich die Onlinezugriffeist der Effekt einer Open-Access-Publikation wesentlich deutlicher als für die IF-relevanten Zitate.…”

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2018

Klin Monatsbl Augenheilkd

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Photorezeptortransplantation in die degenerative Netzhaut

Cited by 4 publications

References 71 publications

Generation of a Cone Photoreceptor-specific GNGT2 Reporter Line in Human Pluripotent Stem Cells

Generation of a Cone Photoreceptor-specific GNGT2 Reporter Line in Human Pluripotent Stem Cells

Cell Ferroptosis: New Mechanism and New Hope for Retinitis Pigmentosa

Thieme Open und Open Access (OA)

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