Photoreceptor Degeneration in Mice: Adeno-Associated Viral Vector-Mediated Delivery of Erythropoietin

Colella, Pasqualina; Auricchio, Alberto

doi:10.1007/978-1-62703-308-4_16

Cited by 2 publications

(2 citation statements)

References 52 publications

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“…Pre-clinical studies demonstrated that recombinant AAV vectors have been a promising tool to deliver EPO to the retina. Colella and Auricchio 94 described a method using AAV viral serotype 2 to deliver EPO subretinally in a light-damage model of induced retinal degeneration and observed successful neuroprotective effect. They used non-erythropoietic EPO derivatives, which did not increase hematocrit level.…”

Section: Formulation Of Epo For the Treatment In Ocular Disordersmentioning

confidence: 99%

Revisiting the role of erythropoietin for treatment of ocular disorders

Ding

Leow

Munisvaradass

et al. 2016

Eye

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Erythropoietin (EPO) is a glycoprotein hormone conventionally thought to be responsible only in producing red blood cells in our body. However, with the discovery of the presence of EPO and EPO receptors in the retinal layers, the EPO seems to have physiological roles in the eye. In this review, we revisit the role of EPO in the eye. We look into the biological role of EPO in the development of the eye and the physiologic roles that it has. Apart from that, we seek to understand the mechanisms and pathways of EPO that contributes to the therapeutic and pathological conditions of the various ocular disorders such as diabetic retinopathy, retinopathy of prematurity, glaucoma, age-related macular degeneration, optic neuritis, and retinal detachment. With these understandings, we discuss the clinical applications of EPO for treatment of ocular disorders, modes of administration, EPO formulations, current clinical trials, and its future directions.

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Section: Formulation Of Epo For the Treatment In Ocular Disordersmentioning

confidence: 99%

Revisiting the role of erythropoietin for treatment of ocular disorders

Ding

Leow

Munisvaradass

et al. 2016

Eye

View full text Add to dashboard Cite

show abstract

“…Epo was recognized as an engine for blood generation in various hematopoietic and non-hematopoietic mammalian tissues, including the central and peripheral nerve systems. Various experimental studies have shown that Epo exerts a marked neuroprotective effect in vivo and in vitro in nervous system disorders of the brain ( 3 , 4 ), spinal cord ( 5 , 6 ) and retina ( 7 - 9 ), among others.…”

Section: Introductionmentioning

confidence: 99%

Protective effect of adenovirus-mediated erythropoietin expression on the spiral ganglion neurons in the rat inner ear

et al. 2018

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The aim of the present study was to evaluate the expression of erythropoietin (Epo) and the Epo receptor (Epo-R) in the spiral ganglion neurons (SGNs) of the rat inner ear, and to assess the effect of Epo adenovirus vector (Ad-Epo) on the spontaneous apoptosis of SGNs. A total of 60 ears from 30 healthy neonatal (2-3 days postnatal) Sprague-Dawley rats were used to examine the expression of Epo in the SGNs. The rats were divided into three groups: The negative control group, the vector control group [infected with a green fluorescent protein expression vector (Ad-GFP)] and the Ad-Epo group (infected with Ad-Epo). The expression of Epo and Epo-R was detected by immunohistochemistry and dual immunofluorescence staining using polyclonal antibodies directed against Epo and Epo-R, followed by confocal laser-scanning microscopy. An adenovirus vector was constructed and used to transfect the cultured SGNs. Following adenovirus infection, apoptosis of the SGNs was evaluated and Epo protein expression was assessed. Epo and Epo-R were widely expressed in the plasma membrane and the cytoplasm of the SGNs, as well as in the organ of Corti and the stria vascularis within the inner ear. Epo protein expression was upregulated in the Ad-Epo group compared with that in the other two groups (P<0.05). Apoptotic cells were seldom observed at day 4 of SGN culture in the negative control group. At day 7, marked apoptotic cells were detected in the negative control group and the vector control group. The apoptosis level in the Ad-Epo group was significantly decreased compared with that in the negative control group or the vector control group at day 7 (P<0.05). In conclusion, Epo and Epo-R are expressed in the SGNs of the inner ear of the rat, and Ad-Epo can decrease the spontaneous apoptosis of SGNs, which may provide a basis for the prevention or alleviation of sensorineural hearing loss.

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Photoreceptor Degeneration in Mice: Adeno-Associated Viral Vector-Mediated Delivery of Erythropoietin

Cited by 2 publications

References 52 publications

Revisiting the role of erythropoietin for treatment of ocular disorders

Revisiting the role of erythropoietin for treatment of ocular disorders

Protective effect of adenovirus-mediated erythropoietin expression on the spiral ganglion neurons in the rat inner ear

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