Photoreceptor allografts in a feline model of retinal degeneration

Ivert, Lena; Gouras, Peter; Naeser, Peter; Narfström, Kristina

doi:10.1007/s004170050169

Cited by 23 publications

(16 citation statements)

References 12 publications

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“…Initially, a subretinal injection of dissociated neuro‐retinal cells was administered using a one‐port sclerotomy procedure and visualizing the injection through the pupil with a contact lens and an operating microscope 3 . These transplants survived for at least 6 months, but rosette formation was invariably observed.…”

Section: Introductionmentioning

confidence: 99%

Lens sparing pars plana vitrectomy and retinal transplantation in cats

Bragadottir

Narfström

2003

Veterinary Ophthalmology

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Neuroretinal transplantation techniques have been evolving during recent years. Experiments in rodent models with degenerative retinal disease have been encouraging. This paper describes a surgical technique developed for use in the Abyssinian cat mutant. After two-port pars plana vitrectomy, retinotomy and bleb formation, whole sheets of neonatal neuroretinal allografts were placed into the subretinal space. The surgery was difficult but feasible, and the main complication was intraoperative hemorrhage.

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Section: Introductionmentioning

confidence: 99%

Lens sparing pars plana vitrectomy and retinal transplantation in cats

Bragadottir

Narfström

2003

Veterinary Ophthalmology

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“…In an earlier study that used the Abyssinian mutant for retinal transplantation, dissociated neuro‐retinal cells were administered subretinally using a one‐port sclerotomy procedure, without vitrectomy 25 . These transplants survived for at least 6 months, but severe rosette formation was invariably observed.…”

Section: Discussionmentioning

confidence: 99%

“…Using a well‐established large animal model for human recessive RP 24 we wanted to investigate whether intact sheets of feline fetal retina, treated with BDNF microspheres integrate into the dystrophic Abyssinian cat retina and if the transplanted tissue develops normally in vivo . This cat model has been used previously for transplantation of small pieces of 3–5‐day‐old kitten retina to the subretinal space 25 . These transplants developed rosettes.…”

Section: Introductionmentioning

confidence: 99%

Functional and structural assessment of retinal sheet allograft transplantation in feline hereditary retinal degeneration

Seiler¹,

Aramant

Seeliger

et al. 2009

Veterinary Ophthalmology

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“…Such transplanted photoreceptors could survive and maintain their outer segments long-term (Gouras and Tanabe, 2003). However, after transplantation of retinal microaggregates to retinal degenerate cats, photoreceptors mostly formed rosettes (spheres of photoreceptors with outer segments in the center) (Ivert et al, 1998). Rosette formation thus showed some level of laminar organization that could not be achieved with dissociated cells.…”

Section: History Of Retinal Transplantationmentioning

confidence: 99%

Cell replacement and visual restoration by retinal sheet transplants

Seiler

Aramant

2012

Progress in Retinal and Eye Research

139

119

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Retinal diseases such as age-related macular degeneration (ARMD) and retinitis pigmentosa (RP) affect millions of people. Replacing lost cells with new cells that connect with the still functional part of the host retina might repair a degenerating retina and restore eyesight to an unknown extent. A unique model, subretinal transplantation of freshly dissected sheets of fetal-derived retinal progenitor cells, combined with its retinal pigment epithelium (RPE), has demonstrated successful results in both animals and humans. Most other approaches are restricted to rescue endogenous retinal cells of the recipient in earlier disease stages by a ‘nursing’ role of the implanted cells and are not aimed at neural retinal cell replacement. Sheet transplants restore lost visual responses in several retinal degeneration models in the superior colliculus (SC) corresponding to the location of the transplant in the retina. They do not simply preserve visual performance – they increase visual responsiveness to light. Restoration of visual responses in the SC can be directly traced to neural cells in the transplant, demonstrating that synaptic connections between transplant and host contribute to the visual improvement. Transplant processes invade the inner plexiform layer of the host retina and form synapses with presumable host cells. In a Phase II trial of RP and ARMD patients, transplants of retina together with its RPE improved visual acuity. In summary, retinal progenitor sheet transplantation provides an excellent model to answer questions about how to repair and restore function of a degenerating retina. Supply of fetal donor tissue will always be limited but the model can set a standard and provide an informative base for optimal cell replacement therapies such as embryonic stem cell (ESC)-derived therapy.

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Photoreceptor allografts in a feline model of retinal degeneration

Cited by 23 publications

References 12 publications

Lens sparing pars plana vitrectomy and retinal transplantation in cats

Lens sparing pars plana vitrectomy and retinal transplantation in cats

Functional and structural assessment of retinal sheet allograft transplantation in feline hereditary retinal degeneration

Cell replacement and visual restoration by retinal sheet transplants

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