Functional and structural assessment of retinal sheet allograft transplantation in feline hereditary retinal degeneration

Seiler, Magdalene J.; Aramant, Robert B.; Seeliger, Mathias W.; Bragadóttir, Ragnheiður; Mahoney, Melissa J.; Narfström, Kristina

doi:10.1111/j.1463-5224.2009.00693.x

Cited by 22 publications

(24 citation statements)

References 54 publications

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“…However, it was revealed that the clonogenic spheres derived from the mouse and human CE originate from differentiated pigmented CE cells rather than the stem cell population harbored in the CE [5]. Fetal cat retinal sheets incubated with BDNF microspheres were transplanted to the subretinal space to investigate whether it is possible for sheets of fetal retinal allograft to integrate into the dystrophic Abyssinian cat retina where progressive rod cone degeneration is taking place [6]. To the authors knowledge there is a lack of information regarding generation, proliferation, and differentiation of progenitor cells during embryonic and fetal stages in other mammals, particularly in the dog where progressive retinal degeneration associated with different ocular diseases is frequently observed.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Progenitor Cells during Canine Retinal Development

Ávila-García

García-Sánchez

Lira-Romero

et al. 2012

Stem Cells International

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We identify the presence of progenitor cells during retinal development in the dog, as this species represents a natural model for studying several breed-specific degenerative retinal disorders. Antibodies to detected progenitor cells (Pax6, C-kit, and nestin) and ganglion cells (BDNF, Brn3a, and Thy1) were used in combination with H3 for the purpose of identifying proliferating cells. Pax6, nestin, C-kit, and H3 were localized mainly in the neuroblastic layer of the retina during the embryonic stage. During the fetal stage, proteins were expressed in the inner neuroblastic layer (INL) as well as in the outer neuroblastic layer; BDNF, Thy1, and Brn3a were also expressed in the INL. During the neonatal stage only C-kit was not expressed. Proliferating cells were present in both undifferentiated and differentiated retina. These results suggest that, during canine retinogenesis, progenitor cells are distributed along the retina and some of these cells remain as progenitor cells of the ganglion cells during the first postnatal days.

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Section: Introductionmentioning

confidence: 99%

Characterization of Progenitor Cells during Canine Retinal Development

Ávila-García

García-Sánchez

Lira-Romero

et al. 2012

Stem Cells International

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“…Transplantation of full-thickness retina has been reported in several animal models previously [6–8,14]. In earlier work in pig and rabbit eyes, we used a siliconized glass cannula for transplantation in which the elastic retinal graft adopted a rolled-up shape.…”

Section: Discussionmentioning

confidence: 99%

“…To improve graft survival and organization, transplantation of non-disrupted tissue in the form of full-thickness retina has been explored by a few groups [5–8]. The rationale of the full-thickness paradigm is to limit antigen exposure to the host immune system, and retain the laminated organization of the donor retina with its delicate intrinsic microcircuitry.…”

Section: Introductionmentioning

confidence: 99%

Retinal transplantation using surface modified poly(glycerol-co-sebacic acid) membranes

et al. 2010

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In retinal transplantation experiments it is hypothesized that remaining diseased photoreceptor cells in the host retina and inner retinal cells in transplants physically obstruct the development of graft-host neuronal contacts which are required for vision. Recently, we developed methods for the isolation of donor photoreceptor layers in vitro, and the selective removal of host photoreceptors in vivo using biodegradable elastomeric membranes composed of poly(glycerol-co-sebacic acid) (PGS). We also coated PGS membranes with electrospun nanofibers, composed of laminin and poly(epsilon-caprolactone) (PCL), to promote attachment of embryonic retinal explants, allowing the resulting composites to be handled surgically as a single entity. Here, we report subretinal transplantation of these composites into adult porcine eyes. In hematoxylin and eosin stained sections of composite explants after 5–7 days in vitro, excellent fusion of retinas and biomaterial membranes was noted, with the immature retinal components showing laminated as well as folded and rosetted areas. The composite grafts could be transplanted in all cases and, 3 months after surgery, eyes displayed clear media, attached retinas and the grafts located subretinally. Histological examination revealed that the biomaterial membrane had degraded without any signs of inflammation. Transplanted retinas displayed areas of rosettes as well as normal lamination. In most cases inner retinal layers were present in the grafts. Laminated areas displayed well-developed photoreceptors adjacent to an intact host retinal pigment epithelium and degeneration of the host outer nuclear layer (ONL) was often observed together with occasional fusion of graft and host inner layers.

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“…Other therapy approaches for which the CEP290-mutant cat has been used include the following: transplantation of sheets of allogenic fetal retinal tissue [21], and of brain derived neural precursor cells [22] into the subretinal space. In the latter study retinal incorporation of some of the injected cells was detected.…”

Section: Cep290-mutant Catmentioning

confidence: 99%

Drug and gene therapy of hereditary retinal disease in dog and cat models

Petersen‐Jones

2013

Drug Discovery Today: Disease Models

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Functional and structural assessment of retinal sheet allograft transplantation in feline hereditary retinal degeneration

Cited by 22 publications

References 54 publications

Characterization of Progenitor Cells during Canine Retinal Development

Characterization of Progenitor Cells during Canine Retinal Development

Retinal transplantation using surface modified poly(glycerol-co-sebacic acid) membranes

Drug and gene therapy of hereditary retinal disease in dog and cat models

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