Photoprotective potential of medicinal plants from Cerrado biome (Brazil) in relation to phenolic content and antioxidant activity

Nunes, Alesandra Ricardo; Rodrigues, Ana Livya Moreira; Queiróz, Dinalva Brito de; Vieira, Ícaro Gusmão Pinto; Neto, João Francisco Câmara; Júnior, João Tavares Calixto; Tintino, Saulo Relison; Morais, Selene Maia de; Coutinho, Henrique Douglas Melo

doi:10.1016/j.jphotobiol.2018.10.013

Cited by 44 publications

(33 citation statements)

References 27 publications

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“…UVB also induces increased formation of reactive oxygen species (ROS) capable of activating the phosphorylation of p53 and inhibiting DNA repair [8]. Cells can protect themselves against the production of UVB-induced ROS by producing several endogenous and exogenous antioxidants [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Protective effects of novel derivatives of vitamin D3 and lumisterol against UVB-induced damage in human keratinocytes involve activation of Nrf2 and P53 defense mechanisms

Chaiprasongsuk

Kim

Holick

et al. 2018

Free Radical Biology and Medicine

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We tested whether novel CYP11A1-derived vitamin D 3-and lumisterol-hydroxyderivatives, including 1,25(OH) 2 D 3 , 20(OH)D 3 , 1,20(OH) 2 D 3 , 20,23(OH) 2 D 3 , 1,20,23(OH) 3 D 3 , lumisterol, 20(OH)L 3 , 22(OH)L 3 , 20,22(OH) 2 L 3 , and 24(OH)L 3 , can protect against UVB-induced damage in human epidermal keratinocytes. Cells were treated with above compounds for 24 h, then subjected to UVB irradiation at UVB doses of 25, 50, 75, or 200 mJ/cm 2 , and then examined for oxidant formation, proliferation, DNA damage, and the expression of genes at the mRNA and protein levels. Oxidant formation and proliferation were determined by the DCFA-DA and MTS assays, respectively. DNA damage was assessed using the comet assay. Expression of antioxidative genes was evaluated by real-time RT-PCR analysis. Nuclear expression of CPD, phospho-p53, and Nrf2 as well as its target proteins including HO-1, CAT, and MnSOD, were assayed by immunofluorescence and western blotting. Treatment of cells with the above compounds at concentrations of 1 or 100 nM showed a dose-dependent reduction in oxidant formation. At 100 nM they inhibited the proliferation of cultured keratinocytes. When keratinocytes were irradiated with 50-200 mJ/cm 2 of UVB they also protected against DNA damage, and/or induced DNA repair by enhancing the repair of 6-4PP and attenuating CPD levels and the tail moment of comets. Treatment with test compounds increased expression of Nrf2-target genes involved in the antioxidant response including GR, HO-1, CAT, SOD1, and SOD2, with increased protein expression for HO-1, CAT, and MnSOD. The treatment also stimulated the phosphorylation of p53 at Ser-15, increased its concentration in the nucleus and enhanced Nrf2 translocation into the nucleus. In conclusion, pretreatment of keratinocytes with 1,25(OH) 2 D 3 or CYP11A1-derived vitamin D 3-or lumisterol hydroxy-derivatives, protected them against UVB-induced damage via activation of the Nrf2-dependent antioxidant response and p53-phosphorylation, as well as by the induction of the DNA repair system. Thus, the new vitamin D 3 and lumisterol hydroxy-derivatives represent promising anti-photodamaging agents.

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Section: Introductionmentioning

confidence: 99%

Protective effects of novel derivatives of vitamin D3 and lumisterol against UVB-induced damage in human keratinocytes involve activation of Nrf2 and P53 defense mechanisms

Chaiprasongsuk

Kim

Holick

et al. 2018

Free Radical Biology and Medicine

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“…Tabela 2: Espécie, nome popular, produto extraído e os principais princípios ativos identificado nas plantas para controle de acantocéfalos no tambaqui. Aureus (NUNES et al, 2018). Estes autores relatam que esta atividade da L. microphylla pode estar relacionada provavelmente à presença dos monoterpenos β-ocimeno e 1,8-cineole, compostos que possuem capacidade de penetrar as membranas celulares, alterando sua função, causando inchaço e aumentando a permeabilidade da membrana citoplasmática.…”

Section: Resultsunclassified

Potencial de Lippia microphylla cham. (verbenaceae) como tratamento alternativo para acantocefalose no tambaqui (Colossoma macropomum)

Jesus

Pereira

2020

BJD

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“…Bulla et al (2015), analysed the in vitro photoprotective potential and the in vivo percutaneous Other plant species also showed photoprotective action. Nunes et al (2018) demonstrated that the ethanolic extracts of the leaves and barks of Amburana cearenses and Curatella americana presented values of SPF of 12.60-14.74, at concentration of 0.2 mg mL −1 , below the SPF value found for the present peel extract, at the same concentration (24.84 ± 0.76, Table 3), and the ethanolic extract of Camellia sinensis (Kaur and Saraf, 2011), which presented a value of SPF 18.10 ± 0.05 at the same concentration. Silva et al (2016) investigated the crude peptone extract of Spondias purpurea at 10 mg mL -1 and obtained SPF values of the formulations prepared, with the extracts in 10, 20 and 30 % of 4.13, 7.82 and 13.99, respectively, inferior to the present data (Table 3).…”

Section: In Vitro Sun Protection Factor (Spf) Of the Crude Extracts Amentioning

confidence: 99%

Schinus terebenthifolius Raddi extracts: From sunscreen activity toward protection of the placenta to Zika virus infection, new uses for a well-known medicinal plant

Oliveira

Valentim

Rocha

et al. 2020

Industrial Crops and Products

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Schinus terebinthifolius Raddi is a well-known medicinal plant native of South America. This species has demonstrated important biological activities such as antihypertensive and vasodilator, antimicrobial, anti-inflammatory and antioxidant. However, no studies have been, so far, reported with the fruits of S. terebinthifolius as a protector of the placenta against Zika virus infection and as sunscreen agents. The present study aimed to investigate new uses for the ethanolic fruit extracts of S. terebinthifolius, from fruits'peel (STPE) and from the whole fruits (STWFE). Zika virus (ZIKV) has been linked to several fetal malformations, such as microcephaly and other central nervous system abnormalities. Thus, the potential of these natural extracts against ZIKV infection was evaluated, using an in vitro method. The photoprotective potential, determined by spectrometry, along with phenolic content, antioxidant capacity, and chemical composition of both extracts were also evaluated. The chemical composition of the extracts was evaluated by HPLC-UV / vis. The cytotoxicity of peel and whole fruit extracts in vero E6 cell lines, in placental cell lines and placental explant cultures were evaluated by the MTT assay. The infectivity of placental cells and explants was evaluated by qRT-PCR and the effects of extracts on ZIKV infection were investigated using HTR-8/SVneo cells, pre-treated with 100 μg mL −1 of STWFE for 1 h, and infected with MR766 (AD) or PE243 (EH) ZIKV strains. STFE and STWFE were well-tolerated by both placental-derived trophoblast cell line HTR-8/SVneo as well as by term placental chorionic villi explants, which indicate absence of cytotoxicity in all analysed concentrations. Two strains of ZIKV were tested to access if pretreatment of trophoblast cells with the STWFE would protect them against infection. Flow cytometry analysis revealed that STWFE extract greatly reduced ZIKV infection. The extracts were also photoprotective with SPF values equivalent to the standard, benzophenone-3. The formulations prepared in different concentrations of the extracts (5-10 %) had shown maximum SPF values of 32.21. STWFE represents a potential natural mixture to be used in pregnancy in order to restrain placental infection by ZIKV and might potentially protect fetus against ZIKV-related malformations. The extracts exhibited photoprotective activity and some of the phenolic compounds, mainly resveratrol, catechin and epicatechin, are active ingredients in all assayed activities. The development of biotechnological/medical products, giving extra value to products from family farming, is expected, with strong prospects for success.

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Photoprotective potential of medicinal plants from Cerrado biome (Brazil) in relation to phenolic content and antioxidant activity

Cited by 44 publications

References 27 publications

Protective effects of novel derivatives of vitamin D3 and lumisterol against UVB-induced damage in human keratinocytes involve activation of Nrf2 and P53 defense mechanisms

Protective effects of novel derivatives of vitamin D3 and lumisterol against UVB-induced damage in human keratinocytes involve activation of Nrf2 and P53 defense mechanisms

Potencial de Lippia microphylla cham. (verbenaceae) como tratamento alternativo para acantocefalose no tambaqui (Colossoma macropomum)

Schinus terebenthifolius Raddi extracts: From sunscreen activity toward protection of the placenta to Zika virus infection, new uses for a well-known medicinal plant

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