Photophosphorylation activity in cell-free preparations of a blue-green alga

Duane, Warren C.; Hohl, Mary C.; Krogmann, David W.

doi:10.1016/0926-6585(65)90095-6

Cited by 34 publications

(3 citation statements)

References 10 publications

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“…Although the blue-green algae are morphologically simple, their level of metabolic complexity is quite high. For example, their photosynthetic mechanism is similar to that observed in the higher plants: two photoacts are necessary and the high potential oxygen evolution mechanism is present (3,8).…”

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confidence: 76%

Respiration in Blue-Green Algae

Biggins

1969

J Bacteriol

140

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The low rate of endogenous respiration exhibited by the blue-green algae Anacystis nidulans and Phormidium luridum was not increased by the addition of respiratory substrates. However, endogenous respiration was inhibited by low concentrations of cyanide and by high carbon monoxide tensions. In addition, the uncouplers dinitrophenol and carbonyl cyanide p-trifluoromethoxyphenylhydrazone both stimulated the respiratory rate. The transition of cells from the aerobic steady state to anaerobiosis was accompanied by a decrease in the concentration of cellular nicotinamide adenine dinucleotide phosphate (NADP+) and adenosine triphosphate (ATP), whereas the concentration of nicotinamide adenine dinucleotide (NAD+) was unchanged. Concomitant with the metabolite decreases were stoichiometric increases io reduced NADP+ (NADPH), adenosine diphosphate, and adenosine monophosphate. A decrease in ATP was also observed after the addition of uncouplers. These data are interpreted as evidence for the association of oxidative phosphorylation with the oxidation of NADP+-linked substrates in these algae. Membrane fragments isolated from the algal cells oxidized succinate, malate, ferrocytochrome c, ascorbate-tetramethyl-p-phenylenediamine, and reduced 2,6dichlorophenol indophenol but did not oxidize NADPH or reduced NAD+ in a cyanide-sensitive system. Oxidative phosphorylation has not yet been demonstrated in these fragments, but a dark ATP-Pi exchange, distinct from the lighttriggered exchange associated with photosynthesis, is readily observed. This exchange was inhibited by phloridzin, Atabrine, and uncouplers in concentrations which suggest that the mechanism of oxidative phosphorylation in blue-green algae is different from that found in other bacteria and in mitochondria. These results led to the conclusion that the biochemical basis for obligate autotrophy in these organisms does not lie in the metabolic events associated with terminal electron transport and energy conservation.

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confidence: 76%

Respiration in Blue-Green Algae

Biggins

1969

J Bacteriol

140

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“…chlorophyll a and d-carotene, as well as the Blue-green algae are photoautotrophic prokaaccessory pigment c -phycocyanin in common with some eukaryotic algae (30). Both photosystems I and II and related reactions, including the oxygen-evolution mechanism (14,29,147), are operative in blue-green algae, yielding electron flow from H2O to triphosphopyridine nucleotide (oxidized form) and the accompanying noncyclic photophosphorylation. It is possible to inhibit photosystem II by 3-(3, 4-dichlorophenyl)-1, 1-dimethylurea (DCMU) in blue-green algal cell extracts and to obtain only cyclic photophosphorylation (147).…”

Section: Interaction Between Cyanophage Development and The Photoautomentioning

confidence: 99%

Cyanophages-viruses attacking blue-green algae.

Padan¹,

Shilo²

1973

Bacteriological Reviews

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“…Sucrose-washed particles containing only a trace of phycocyanin retain a high specific Hill reaction activity, although in the presence of phycocyanin, higher rates were found under light-limited conditions (190,743). Corresponding particles from A. nidulans, prepared with dextran or carbowax, lose Hill reaction activity (with ferricyanide, indophenol dyes, or cytochrome c) upon extraction with water, and regain that activity upon readdition of the extract.…”

Section: Fig 10 Polyhedral Body Frommentioning

confidence: 99%