Cyanophages-viruses attacking blue-green algae.

Padan, E; Shilo, Moshe

doi:10.1128/mmbr.37.3.343-370.1973

Cited by 81 publications

(28 citation statements)

References 93 publications

(167 reference statements)

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“…Despite the early isolation of cyanophages infecting filamentous cyanobacteria (Safferman and Morris, 1963), studies attempting to elucidate their impact on the functions of the host organisms or their ecological role are scarce. Although interactions between freshwater filamentous cyanobacteria and their phages have been studied before (Padan and Shilo, 1973), only a few studies have been conducted on the interactions between filamentous, nitrogen-fixing and toxinproducing cyanobacteria and their viral parasites in the Baltic Sea. Jenkins and Hayes (2006) isolated and analysed the morphology and host range of 17 myo-and siphoviruses infecting Nodularia spumigena.…”

Section: Introductionmentioning

confidence: 99%

Newly isolated Nodularia phage influences cyanobacterial community dynamics

Coloma

Dienstbier

Bamford

et al. 2017

Environmental Microbiology

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Cyanophages, that is, viruses infecting cyanobacteria, are a key component driving cyanobacterial community dynamics both ecologically and evolutionarily. In addition to reducing biomass and influencing the genetic diversity of their host populations, they can also have a wider community-level impact due to the release of nutrients by phage-induced cell lysis. In this study, we isolated and characterized a new cyanophage, a siphophage designated as vB_NpeS-2AV2, capable of infecting the filamentous nitrogen fixing cyanobacterium Nodularia sp. AV2 with a lytic cycle between 12 and 18 hours. The role of the phage in the ecology of its host Nodularia and competitor Synechococcus was investigated in a set of microcosm experiments. Initially, phage-induced cell lysis decreased the number of Nodularia cells in the cultures. However, around 18%-27% of the population was resistant against the phage infection. Nitrogen was released from the Nodularia cells as a consequence of phage activity, resulting in a seven-fold increase in Synechococcus cell density. In conclusion, the presence of the cyanophage vB_NpeS-2AV2 altered the ecological dynamics in the cyanobacterial community and induced evolutionary changes in the Nodularia population, causing the evolution from a population dominated by susceptible cells to a population dominated by resistant ones.

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Section: Introductionmentioning

confidence: 99%

Newly isolated Nodularia phage influences cyanobacterial community dynamics

Coloma

Dienstbier

Bamford

et al. 2017

Environmental Microbiology

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“…However, LPP-cyanophages have been found only in freshwater. Furthermore, their morphology is different from that of the phage reported here; the tail of LPP-cyanophages is very short (<50 nm, Padan and Shilo 1973). Safferman et al (1983) proposed a classification of cyanophages according to morphological characteristics following the principles for classification and nomenclature of bacteriophages.…”

Section: Discussionmentioning

confidence: 61%

OCCURRENCE OF A TEMPERATE CYANOPHAGE LYSOGENIZING THE MARINE CYANOPHYTE PHORMIDIUM PERSICINUM¹

Ohki

Fujita

1996

Journal of Phycology

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A temperate cyanophage was found to lysogenize the marine cyanophyte Phormidium persicinum (Reinke) Com. (Provasoli strain). The lytic cycle was induced by the addition of mitomycin C or by brief illumination with ultraviolet light. The lytic process observed under the electron microscope showed that phage particles appeared in a nucleoplasm region 15 to 24 h after the addition of mitomycin C. The induction of the lytic process occurred simultaneously in almost all cells of every trichome. Matured phage particles were released to the medium 30 to 50 h after the addition of mitomycin C. Phage particles isolated from algal lysates had a polyhedral head (about 40 nm in diameter) with a long (about 300 nm) and noncontractile tail. The most abundant protein, presumably a structural protein, had an apparent molecular mass of about 38 kDa. The genome size estimated from restriction analysis was about 50 kbp. Phage DNA was digested with several restriction endonucleases including Sau3AI and DpnI. However, MboI failed to digest the phage DNA, suggesting that the phage DNA is highly methylated. Southern blot analysis suggested that some part of the phage was in the lytic cycle in algal cells growing under normal conditions. A possible role of temperate cyanophages in the regulation of cyanophyte populations in the marine environment is discussed.

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“…The cyanophages are viruses that infect cyanobacteria (Diener, 1963;Ginzburg, Padan & Shilo, 1968;SafFerman et al, 1969; for review see Padan & Shilo, 1973). Very little is known about these viruses; there are relatively few isolates and none of them has been studied in detail.…”

Section: Introductionmentioning

confidence: 99%

Oriented degradation of the genome of cyanophage AN‐23 after exposure to NaCl

Bancroft

Smith

1992

New Phytologist

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SUMMARYThe low tolerance of cyanophage AN-23 to NaCl at more than 0-05 M has been investigated. When isolated from clarified lysates that had been exposed to NaCl in the presence of added DNAse I, the phage genomic DNA consisted of a series of discrete fragments. The fragments were recovered from low melting temperature agarose electrophoresis gels and used for restriction analysis. Comparison of the restriction cleavage products with a restriction map of the 46 kbp AN-23 genome has shown that the fragments are generated by removal of DNA from one end of the linear genome in steps of about 3 kbp. The data are consistent with a model in which NaCl triggers the ejection of the phage genome from the capsid.

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Cyanophages-viruses attacking blue-green algae.

Abstract: All results from Safferman et al. (106). Goldstein et al. (40). Schneider et al. (113). d Adolph and Haselkorn (4). e Safferman et al. (112). ' Luftig and Haselkorn (79). g Mackenzie and Haselkorn (83). Luftig and Haselkorn (77). Safferman and Morris (108).

Cited by 81 publications

References 93 publications

Newly isolated Nodularia phage influences cyanobacterial community dynamics

Newly isolated Nodularia phage influences cyanobacterial community dynamics

OCCURRENCE OF A TEMPERATE CYANOPHAGE LYSOGENIZING THE MARINE CYANOPHYTE PHORMIDIUM PERSICINUM¹

Oriented degradation of the genome of cyanophage AN‐23 after exposure to NaCl

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Cyanophages-viruses attacking blue-green algae.

Abstract: All results from Safferman et al. (106). Goldstein et al. (40). Schneider et al. (113). d Adolph and Haselkorn (4). e Safferman et al. (112). ' Luftig and Haselkorn (79). g Mackenzie and Haselkorn (83). Luftig and Haselkorn (77). Safferman and Morris (108).

Cited by 81 publications

References 93 publications

Newly isolated Nodularia phage influences cyanobacterial community dynamics

Newly isolated Nodularia phage influences cyanobacterial community dynamics

OCCURRENCE OF A TEMPERATE CYANOPHAGE LYSOGENIZING THE MARINE CYANOPHYTE PHORMIDIUM PERSICINUM1

Oriented degradation of the genome of cyanophage AN‐23 after exposure to NaCl

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OCCURRENCE OF A TEMPERATE CYANOPHAGE LYSOGENIZING THE MARINE CYANOPHYTE PHORMIDIUM PERSICINUM¹