Photopatterned materials in bioanalytical microfluidic technology

Tentori, Augusto M.; Herr, Amy E.

doi:10.1088/0960-1317/21/5/054001

Cited by 15 publications

(17 citation statements)

References 65 publications

(88 reference statements)

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“…Contact photolithography has been used to photopolymerize hydrogels [32] to create permeable plugs or microchamber walls [33][34][35][36][37]. At standard device thicknesses, contact lithography exhibits limited resolution [38], which was overcome by projecting shaped laser beams into microchannels using custom optics [38][39][40].…”

Section: Previous Workmentioning

confidence: 99%

Microfluidic Microdialysis: Spatiotemporal Control over Solution Microenvironments Using Integrated Hydrogel Membrane Microwindows

et al. 2013

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We present a powerful and versatile technique that enables exquisite spatial and temporal control over local solution chemistry in microfluidic devices. Using a microscope and a UV lamp, we use projection lithography to photopolymerize thin (10-25 m) hydrogel membrane ''microwindows'' (HMMs) into standard microfluidic devices. These microwindows are permeable to solute and solvent diffusion and to electric fields, yet act as rigid walls from the standpoint of fluid flow. Reservoirs of solution may thus be rapidly imposed, switched, and maintained on one side of a HMM using standard microfluidic techniques, provoking changes in solution conditions on the other side without active mixing, stirring, or diluting. We highlight three paradigmatic experimental capabilities enabled by HMMs: (1) rapid dialysis and swapping of solute and/or solvent, (2) stable and convection-free localized concentration gradients, and (3) local electric permeability. The functional versatility of hydrogel microwindow membranes, coupled with the ease and speed of their fabrication and integration into simple microchannels or multilayer devices, will open a variety of novel applications and studies in a broad range of fields.

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Section: Previous Workmentioning

confidence: 99%

Microfluidic Microdialysis: Spatiotemporal Control over Solution Microenvironments Using Integrated Hydrogel Membrane Microwindows

et al. 2013

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“…Interested readers should consult the reviews published on this topic for photopatterning 123 and immobilization. …”

Section: 121mentioning

confidence: 99%

Polymer sieving matrices in microanalytical electrophoresis

Chung

Kim

Herr

2014

Analyst

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Microfluidic design has advanced existing protein separation capabilities and supported novel assays. Key metrics for successful protein separations include fast, robust, and sensitive analysis of complex mixtures of bio-macromolecules. Attaining high separation resolution is a chief concern. Here we review recent advances in polymer-based electrophoresis sieving materials that are impacting microfluidic bioanalytical applications. Looking forward, we comment on unmet needs for advanced separation media in microto-nanoscale devices.

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“…Photopatterning enables the modification of channel surfaces with a high degree of spatial precision. Photografting and photolinker chemicals can be used inside completely enclosed microchannels which are made of light‐permeable materials such as glass or plastics . In addition, photopatterning technology provides a more convenient, quicker and more accurate positioning method to fabricate polymeric films in order to prepare spatially localized enzyme microchips capable of directional synthesis in comparison to conventional methods.…”

Section: Introductionmentioning

confidence: 99%

Enzyme immobilization on photopatterned temperature‐response poly (N‐isopropylacrylamide) for microfluidic biocatalysis

Zhu

Xiong

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND Microfluidic chips have gained growing attention from the scientific community due to their large surface/interface area and fast mass transfer. However, their application is challenged by the instability of enzymes and time‐consuming catalytic process. Poly (N‐isopropylacrylamide) (PNIPAAm) coupled with photopatterning technology was applied to immobilize an enzyme on the microchannel surface to realize the biotransformation in this study. RESULTS The photopattern‐immobilized naringinase on a microchip achieved a yield of 93.63 ± 1.12% for isoquercitrin production within 5 min. The production of unit time per unit enzyme (g g−1 h−1) increased 2.1‐fold whereas the reaction time decreased to 1/12 of the time required in a batch reactor. The enzyme was absorbed and desorbed at 40 and 25 °C, respectively, and the release efficiency of immobilized naringinase reached 80.57%, indicating that most of the enzymes were replaced with fresh enzymes to proceed each enzymatic reaction. Six cycles of enzymatic hydrolysis reactions were completed successively, maintaining >60% of relative enzymatic activity. CONCLUSION The results indicated that using the immobilized enzyme on the photopatterned PNIPAAm in the enzymatic hydrolysis of rutin was an efficient and simple way to achieve a high yield of isoquercitrin. Thus, this approach represents a convenient and cost‐saving method to produce fine chemicals using microfluidic biocatalysis. © 2019 Society of Chemical Industry

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Photopatterned materials in bioanalytical microfluidic technology

Cited by 15 publications

References 65 publications

Microfluidic Microdialysis: Spatiotemporal Control over Solution Microenvironments Using Integrated Hydrogel Membrane Microwindows

Microfluidic Microdialysis: Spatiotemporal Control over Solution Microenvironments Using Integrated Hydrogel Membrane Microwindows

Polymer sieving matrices in microanalytical electrophoresis

Enzyme immobilization on photopatterned temperature‐response poly (N‐isopropylacrylamide) for microfluidic biocatalysis

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