Photon flux density and wavelength influence on growth, photosynthetic pigments and volatile organic compound accumulation in Aeollanthus suaveolens (Catinga-de-mulata) under in vitro conditions

Araújo, Diene Xavier; Rocha, Tainá Teixeira; Carvalho, Alexandre Alves de; Bertolucci, Suzan Kelly Vilela; Medeiros, Ana Paula Ribeiro; Ribeiro, F. N. S.; Barbosa, Suzana Marques; Pinto, José Eduardo Brasil Pereira

doi:10.1016/j.indcrop.2021.113597

Cited by 18 publications

(7 citation statements)

References 50 publications

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“…For green light this relationship was also confirmed in terms of the content of chlorophyll b (Fan et al 2013). Green and yellow light favored the production of photosynthetic pigments in Aeollanthus suaveolens (Araújo et al 2021) and Lippia dulcis (Rocha et al 2022). Our study demonstrated an increased production of photosynthetic pigments under green LED (RBG).…”

Section: Effect Of Spectral Combination On Photosynthetic Pigment Con...supporting

confidence: 77%

Adventitious organogenesis and phytochemical composition of Madonna lily (Lilium candidum L.) in vitro modeled by different light quality

Pałka

Cioć

Hura

et al. 2022

Plant Cell Tiss Organ Cult

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Lilium candidum L. is a plant with rich cultural traditions and huge potential in floriculture and modern medicine. Our study analyzed the effects of light emitting diodes (LEDs) of variable quality on adventitious organogenesis induced in bulb scales placed on Murashige and Skoog medium. In addition to the spectral compositions of red (100%), blue (100%), and red and blue light (RB ratio 7:3), RB light was mixed in equal proportions (50%) with green (RBG), yellow (RBY), UV (RBUV), and far red (RBfR) light. We also tested white LED (Wled). Fluorescent lamp light (Fl) and darkness (D) served as controls. We assessed the effects of light on the morphometric features as well as the physiological and biochemical quality of the bulblets (adventitious bulbs). Bulb formation was observed under all light qualities, even in darkness, albeit to a limited extent. White LED light (Wled) treatment translated into the highest efficiency of bulblet formation and the greatest number of bulbs with developed leaves. The leaves, even though the shortest ones, also developed as a result of RBG treatment, and this light mixture enhanced the diameter of the forming bulblets. The bulbletsunder this light spectrum accumulated the most chlorophyll and carotenoids. The bulblets formed under B, RBfR and RBG LED had more than two times higher phenoliclevels than those formed under R LED and Fl. The bulbletsformed under B LED were the richest in soluble sugars, similarly to those developing in darkness (D).

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Section: Effect Of Spectral Combination On Photosynthetic Pigment Con...supporting

confidence: 77%

Adventitious organogenesis and phytochemical composition of Madonna lily (Lilium candidum L.) in vitro modeled by different light quality

Pałka

Cioć

Hura

et al. 2022

Plant Cell Tiss Organ Cult

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“…Silva et al (2017) reported that the highest carvacrol content and growth parameters of Plectranthus amboinicus were obtained when the plantlets were cultivated with one or two porous membranes. Araújo et al (2021) showed also that in Aeollanthus suaveolens the accumulation of VOCs was in uenced by photon ux density and spectral quality.…”

Section: Effect the Number Of Membranes On Vocs By Gc/msmentioning

confidence: 94%

Volatile organic compound (VOC) profile and plantlet growth of Aeollanthus suaveolens under conventional and alternative membrane systems

Araújo

Rocha

Carvalho

et al. 2023

Plant Cell Tiss Organ Cult

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In conventional in vitro culture, plantlets are kept in closed containers to avoid contamination and drying of the explant. Ventilation inside the containers improves plant growth and affects secondary metabolism, as it modi es the microenvironmental conditions. Therefore, the objective of this study was to evaluate the effect of the use of ventilation systems on the growth characteristics and production of photosynthetic pigments and volatile organic compounds (VOCs) in Aeollanthus suaveolens Mart. ex Spreng. (Lamiaceae) cultured in vitro. Nodal segments containing one pair of leaves were cultured in a conventional system (NMS) and in natural ventilation systems with one (AMS1), two (AMS2) and four (AMS4) porous membranes. At 40 days, the plantlets were evaluated for growth, VOC concentration, and photosynthetic pigment production. The number of porous membranes used in the vial cap affected growth, photosynthetic pigments and VOCs. A higher number of porous membranes (AMS4) led to greater dry weight accumulation, increased production of photosynthetic pigments, and enhanced synthesis of (Z)-β-farnesene. Lower growth and fewer photosynthetic pigments, and increase linalool acetate synthesis were observed in the culture without the use of porous membranes (NMS). The leaf area of plantlets cultivated with the use of four membranes was 3.8 times greater than that of plantlets cultivated without the use of membranes. For the photoautotrophic cultivation of A. suaveolens in vitro, the use of natural ventilation with four membranes is recommended because it promotes better growth, increases the production of photosynthetic pigments and is superior to the conventional sealed system.

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“…According Araújo et al (2021), the LAR expresses the leaf area corresponding to 1 g of dry weight. The results obtained for LAR (0.014 cm 2 mg -1 ) suggest that Lippia dulcis plantlets grown in MS had a larger leaf area that was useful for carrying out photosynthesis, which contributed to gains in LDW shown in Table 2.…”

Section: Growth Analysismentioning

confidence: 99%

Establishment and clonal propagation of Lippia dulcis Trevir. through in vitro single node cultures

Rocha

Araújo

Assis

et al. 2023

PCC&M

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Lippia dulcis Trevir. (Verbenaceae) is a species with medicinal potential that is used as a tranquilizer and to control diabetes in the western region of Pará, Brazil. The objective of this study was to determine the establishment and clonal propagation of Lippia dulcis through single nodal segments grown in different concentrations of salts in a MS (Murashige and Skoog) medium (MS, MS/2, MS/4) and determine the best period for subculturing the species. Nodal segments cultivated in the MS medium showed a higher survival percentage and better development than those grown in more dilute media (MS/2, MS/4). The concentration of the salts affected the dry weight gain of some plant parts. Plantlets cultivated in the MS medium obtained better leaf dry weight and total leaf area than plantlets cultivated in half-strength MS. The half-strength MS medium proved to be most effective for root length growth and root dry weight gainin vitro. The growth curve showed that day 30 was the time for subculturing the species. Starting with only one nodal segment, 3125 plantlets were obtained after sixth months of cultivation at a multiplication rate of five. The plantlets of Lippia dulcis were removed from the flasks and acclimatized with success in a greenhouse.

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Photon flux density and wavelength influence on growth, photosynthetic pigments and volatile organic compound accumulation in Aeollanthus suaveolens (Catinga-de-mulata) under in vitro conditions

Cited by 18 publications

References 50 publications

Adventitious organogenesis and phytochemical composition of Madonna lily (Lilium candidum L.) in vitro modeled by different light quality

Adventitious organogenesis and phytochemical composition of Madonna lily (Lilium candidum L.) in vitro modeled by different light quality

Volatile organic compound (VOC) profile and plantlet growth of Aeollanthus suaveolens under conventional and alternative membrane systems

Establishment and clonal propagation of Lippia dulcis Trevir. through in vitro single node cultures

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