weight. Photosynthetic pigment levels were lower under LED light compared to control lamps. Phenolic acids and flavonoids were identified in M. communis leaf extracts. Myricetin was the major constituent with highest concentration under red LEDs and highest BA level. Keywords Myrtle · Light quality · Photosynthetic pigments · Secondary metabolites · HPLC Abbreviations LED Light emitting diode B 100% blue LED RB 70% red and 30% blue LED R 100% red LED C Control fluorescent light BA 6-Benzyladenine NAA 1-Naphthaleneacetic acid MS Murashige and Skoog medium HPLC High performance liquid chromatography PPFD Photosynthetic photon flux density Communicated by Sergio J. Ochatt.

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A mixture of red and blue light-emitting diodes (LEDs; at a ratio of 7:3, respectively) were used to analyze the effects of different photosynthetic photon flux densities (PPFDs) (40, 80, and 120 µmol m−2 s−1 hereafter known as LED 40, 80, and 120, respectively) on the micropropagation of Gerbera jamesonii Bolus shoots. The experiment also examined the effect of 6-benzyladenine (BA) in 1, 2.5, and 5 µM concentrations in the media. Biometrical observations and analyses of leaf morphometry and photosynthetic pigment content were conducted. Shoot multiplication increased with an increasing BA concentration. A PPFD of 80 µmol m−2 s−1 and 5 µM BA is suggested as efficient for shoot propagation and economically viable. LED 120 increased the leaf blade area and its width, and circularity and elongation ratios. The intensity of light did not affect the fresh weight, which increased at higher BA concentrations (2.5 and 5 μM). The dry weight content decreased with increasing cytokinin concentration; the greatest content was observed on media with 1 µM BA under PPFD 120 µmol m−2 s−1. LED 80 increased the photosynthetic pigments content in the leaves in comparison to the standard intensity of LED 40. Increased BA concentration raises the content of chlorophyll a.

The experiment evaluated the influence of different light qualities and 6-benzyladenine (BA) concentration in a medium on the leaf response of multiplied Gerbera jamesonii Bolus ex Hook. f.‘Big Apple’ shoots. Three different light-emitting diode (LED) spectra—100% blue (B), 100% red (R) and red and blue mixture (7:3, RB)—were used, and a fluorescent lamp was used as a control (Fl). Concentrations of BA in Murashige and Skoog (MS) medium were 1, 2.5 and 5 µM. Leaves developed under 100% blue light had a lower frequency of stomata and a smaller area as compared with those from plants exposed to light with red in spectrum. Under 100% red light, the leaf area and the frequency of stomata increased along with growing concentration of BA in the medium. The thickest mesophyll was spotted in the cross-section of leaves exposed to the blue LED light. Leaves developed under the 100% red light had the thinnest mesophyll layers. Increasing concentration of BA in the medium resulted in enhanced leaf blade thickness. The cross-section of leaf vascular bundles was only half of that in petioles. The leaves under the LED combinations had larger vascular bundles than those under fluorescent light. The highest level of photosynthetic pigments was noticed in the leaves grown under LED R and RB lights. Our study demonstrated that 2.5 µM BA and a mixture of blue and red light provided by LED improved leaf quality during multiplication of gerbera shoots.

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An energy-saving light emitting diode (LED) system allows for adjustment of light quality, which affects plant development and metabolic processes in in vitro cultures. The study investigated the content of endogenous carbohydrates and the condition of the photosynthetic apparatus of Gerbera jamesonii Bolus ex Hook. f. Our aim was to analyze the effects of different LED light qualities—100% red light (R LED), 100% blue (B LED), a mixture of red and blue (7:3) (RB LED), and a fluorescent lamp as a control (Fl)—during the multiplication of axillary shoots. After 40 days, the culture measurements were performed using a non-invasive pulse amplitude modulation (PAM) fluorimeter. Sugar content was assessed with high performance liquid chromatography (HPLC). Two forms of free monosaccharides (glucose and fructose), two sugar alcohol derivatives (inositol and glycerol), and seven forms of free oligosaccharides were identified. Of those, glucose content was the highest. LEDs did not disturb the sugar metabolism in multiplied shoots. Their monosaccharides were three times more abundant than oligosaccharides; the same results were found in plants grown under control light. R light depleted the performance of the photosynthetic apparatus and caused its permanent damage. The RB LED spectrum ensured the most efficient non-photochemical quenching of the photosystem II (PS II) excitation state and high shoot quality.

Light quality is essential in in vitro cultures for morphogenesis process. Light emitting diodes system (LED) allows adjustment as desired and the most appropriate light spectrum. The study analyzed the influence of different LED light quality on the balance of endogenous phytohormones and related compounds (PhRC) in in vitro multiplied axillary shoots of Gerbera jamesonii. Over a duration of 40 days, the shoots were exposed to 100% red light, 100% blue light, red and blue light at a 7:3 ratio with control fluorescent lamps. Every 10 days plant tissues were tested for their PhRC content with the use of an ultra-high performance liquid chromatography (UHPLC). Shoots’ morphometric features were analyzed after a multiplication cycle. We identified 35 PhRC including twelve cytokinins, seven auxins, nine gibberellins, and seven stress-related phytohormones. Compounds content varied from 0.00052 nmol/g to 168.15 nmol/g of dry weight (DW). The most abundant group were stress-related phytohormones (particularly benzoic and salicylic acids), and the least abundant were cytokinins (about 370 times smaller content). LED light did not disturb the endogenous phytohormone balance, and more effectively mitigated the stress experienced by in vitro grown plants than the fluorescent lamps. The stress was most effectively reduced under the red LED. Red and red:blue light lowered tissue auxin levels. Blue LED light lowered the shoot multiplication rate and their height, and induced the highest content of gibberellins at the last stage of the culture.

The tree ferns are an important component of tropical forests. In view of this, the enhancement of in vitro production of these plants is needed. Thus, the effect of different light-emitting diodes (LEDs) as well as control fluorescent lamps (Fl) and a 3-week-long period of darkness at the beginning of in vitro culture on micropropagation of the tree fern Cyathea delgadii Sternb. was analysed. Moreover, the photosynthetic pigment content and secondary metabolite profiles were estimated. The period of darkness contributed to a high production of somatic embryo-derived sporophytes and a low production of gametophytes. The formation of new sporophytes was stimulated by RBY (35% red, 15% blue, and 50% yellow) and B (100% blue) lights when the stipe explants or whole young sporophytes were used in the culture, respectively. The elongation of the roots and leaves was stimulated by RBfR light (35% red, 15% blue, and 50% far red), while root production increased under RBY light. The RB (70% red and 30% blue) and B lights stimulated the accumulation of chlorophyll better than Fl light. The most abundant metabolite found in the plant extracts was trans-5-O-caffeoylquinic acid (1.013 µg/mg of dry weight). The extract obtained from plants growing in a greenhouse had the best antioxidant activity.