Photolithographic patterning of polyethylene glycol hydrogels

Hahn, Mariah S.; Taite, Lakeshia J.; Moon, James J.; Rowland, M. Cannon; Ruffino, Katie A.; West, Jennifer L.

doi:10.1016/j.biomaterials.2005.11.045

Cited by 380 publications

(331 citation statements)

References 18 publications

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“…PEGDA was prepared as previously described [31] by combining 0.1 mmol ml −1 dry PEG (8 or 10 kDa, Fluka), 0.4 mmol ml −1 acryloyl chloride and 0.2 mmol ml −1 triethylamine in anhydrous dichloromethane (DCM) and stirring under argon overnight. The resulting solution was washed with 2 M K 2 CO 3 and separated into aqueous and DCM phases to remove HCl.…”

Section: Polymer Synthesismentioning

confidence: 99%

“…Cell adhesion peptide RGDS (American Peptide) was conjugated to an acrylated PEG derivative (3.4 kDa) by reaction with acryloyl-PEG-N-hydroxysuccinimide (ACRL-PEG-NHS, Nektar) at a 1:1 molar ratio for 2 h in 50 mM sodium bicarbonate buffer, pH 8.5 [31]. The product (ACRL-PEG-RGDS) was purified by dialysis, lyophilized and stored at −20 °C until use.…”

Section: Synthesis Of Acrylate-derivatized Peptidesmentioning

confidence: 99%

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Influence of hydrogel mechanical properties and mesh size on vocal fold fibroblast extracellular matrix production and phenotype

et al. 2008

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Current clinical management of vocal fold (VF) scarring produces inconsistent and often suboptimal results. Researchers are investigating a number of alternative treatments for VF lamina propria (LP) scarring, including designer implant materials for functional LP regeneration. In the present study, we investigate the effects of the initial scaffold elastic modulus and mesh size on encapsulated VF fibroblast (VFF) extracellular matrix (ECM) production toward rational scaffold design. Polyethylene glycol diacrylate (PEGDA) hydrogels were selected for this study since their material properties, including mechanical properties, mesh size, degradation rate and bioactivity, can be tightly controlled and systematically modified. Porcine VFF were encapsulated in four PEGDA hydrogels with degradation half lives of ~25 days and initial elastic compressive moduli ranging from ~30 to 100 kPa and initial mesh sizes ranging from ~9 to 27 nm. After 30 days of static culture, VFF ECM production and phenotype in each formulation was assessed biochemically and histologically. Sulfated glycosaminoglycan synthesis increased in similar degree with both increasing initial modulus and decreasing initial mesh size. In contrast, elastin production decreased with increasing initial modulus but increased with decreasing initial mesh size. Both collagen deposition and the induction of a myofibroblastic phenotype depended strongly on initial mesh size but appeared largely unaffected by variations in initial modulus. The present results indicate that scaffold mesh size warrants further investigation as a critical regulator of VFF ECM synthesis. Furthermore, this study validates a systematic and controlled approach for analyzing VFF response to scaffold properties, which should aid in rational scaffold selection/design.

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Section: Polymer Synthesismentioning

confidence: 99%

Section: Synthesis Of Acrylate-derivatized Peptidesmentioning

confidence: 99%

Influence of hydrogel mechanical properties and mesh size on vocal fold fibroblast extracellular matrix production and phenotype

et al. 2008

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“…To date, three-dimensional micro hydrogel structures have been created by a variety of methods, including photolithography, softlithography (e.g., micro-contact printing, microfluidic patterning, and micro molding) [9]- [11]. However, the performance of these traditional methods is limited by several drawbacks: (1) requirement for lithographic masks to define micro/nano scale structures; (2) extremely difficult if at all possible to obtain high-aspect-ratio structures or structures with variable height on the same substrate.…”

Section: Introductionmentioning

confidence: 99%

3-D Non-UV Digital Printing of Hydrogel Microstructures by Optically Controlled Digital Electropolymerization

Liu

Pan

Liu

et al. 2015

J. Microelectromech. Syst.

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Abstract-A technique using digital masks without ultraviolet light to rapidly print 3-D biopolymer structures with complex microarchitectures in a microfluidic chip has been demonstrated. In this approach, a customized system is used to project light images on a photoconductive substrate in order to create localized virtual electrodes when an alternating electric field is applied across the fluidic medium in an optically controlled digital electropolymerization chip. Upon these virtual electrodes, the localized electric fields are generated, which could activate the polymerization of acrylate-based molecules, such as poly(ethylene glycol) diacrylate (PEGDA), to form microstructures with the same shapes as the projected light images. We have demonstrated that the 3-D PEGDA microstructures with the customized shapes could be fabricated rapidly through a layer-by-layer process by applying a series of digital masks (projected light images). With our current projection and microscopy system, the fabrication of microhydrogel structures with a lateral resolution of 3 µm and an adjustable thickness ranging from tens of nanometers to hundreds of micrometers has been demonstrated. In summary, this novel technique provides an efficient process for the rapid printing of the 3-D biopolymer-based microstructures, and could enable many future applications in a mechanoanalysis of cancer cells, tissue engineering, and drug screening.[2015-0110]

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“…Poly(ethylene glycol)-diacrylate (PEG-DA) was prepared as previously described 25 from PEG (M n = 3400 Da). The resultant polymer had a molecular weight M n = 3676 -16 Da with a polydispersity of 1.088 -0.015 as determined by gel permeation chromatography (Shimadzu), and the presence of conjugated acrylate groups was verified with 1 H-NMR.…”

Section: Polymer Synthesis and Characterizationmentioning

confidence: 99%

Three-Dimensional In Vitro Tri-Culture Platform to Investigate Effects of Crosstalk Between Mesenchymal Stem Cells, Osteoblasts, and Adipocytes

Hammoudi

Rivet

Kemp

et al. 2012

Tissue Engineering Part A

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The bone marrow niche for mesenchymal stem cells (MSCs) contains different amounts of bone and fat that vary with age and certain pathologies. How this dynamic niche environment may affect their differentiation potential and/or healing properties for clinical applications remains unknown, largely due to the lack of physiologically relevant in vitro models. We developed an enabling platform to isolate and study effects of signaling interactions between tissue-scale, laminated hydrogel modules of multiple cell types in tandem. We applied this platform to co-and tri-culture of primary human MSCs, osteoblasts, and adipocytes over 18 days in vitro. Each cell type was analyzed separately with quantitative polymerase chain reaction (qPCR) and histochemistry for several mesenchymal lineage markers. Distinct expression dynamics for osteogenic, adipogenic, chondrogenic, and myogenic transcriptional regulators resulted within each cell type depending on its culture setting. Incorporating this data into multivariate models produced latent identifiers of each emergent cell type dependent on its co-or triculture setting. Histological staining showed sustained triglyceride storage in adipocytes regardless of culture condition, but transient alkaline phosphatase activity in both osteoblasts and MSCs. Taken together, our results suggest novel emergent phenotypes for MSCs, osteoblasts, and adipocytes in bone marrow that are dependent on and result in part from paracrine interactions with their neighboring cell types.

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Photolithographic patterning of polyethylene glycol hydrogels

Cited by 380 publications

References 18 publications

Influence of hydrogel mechanical properties and mesh size on vocal fold fibroblast extracellular matrix production and phenotype

Influence of hydrogel mechanical properties and mesh size on vocal fold fibroblast extracellular matrix production and phenotype

3-D Non-UV Digital Printing of Hydrogel Microstructures by Optically Controlled Digital Electropolymerization

Three-Dimensional In Vitro Tri-Culture Platform to Investigate Effects of Crosstalk Between Mesenchymal Stem Cells, Osteoblasts, and Adipocytes

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