Photokilling of bacteria by the natural dye curcumin

Dahl, Thomas; McGowan, William M.; Shand, Mark A.; Srinivasan, Vinod

doi:10.1007/bf00414437

Cited by 149 publications

(116 citation statements)

References 5 publications

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“…Thus, although promising results were described with the use of CUR without light excitation, higher concentrations of the compound were needed to induce the antifungal effect. Likewise, the studies of Dahl et al [34] and Tønnesen et al [35] reported that the antibacterial activity of some CUR concentrations was greatly enhanced by light. Another finding in the present study was that PDT effectiveness was not always fluence-dependent.…”

Section: Discussionmentioning

confidence: 90%

Susceptibility of clinical isolates of Candida to photodynamic effects of curcumin

et al. 2011

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Background and ObjectiveThe resistance of Candida species to antifungals represents a major challenge for therapeutic and prophylactic strategies. This study evaluated photodynamic therapy (PDT) mediated by Curcumin (CUR) against clinical isolates of C. albicans, C. tropicalis, and C. glabrata, both in planktonic and biofilm forms.Study Design/Materials and MethodsSuspensions of Candida were treated with three CUR concentrations and exposed to four LED fluences. The protocol that showed the best outcomes for inactivation of the planktonic phase was selected to be evaluated against Candida biofilms. In addition, two higher CUR concentrations were tested. The metabolic activity of biofilms was evaluated by means of XTT reduction assay and the biofilm biomass was evaluated using crystal violet (CV) staining assay. Data were analyzed in a mixed model nested ANOVA, Wilcoxon's nonparametric tests, and the Kruskal–Wallis test (α = 5%).ResultsThe use of CUR in association with light was able to promote a significant antifungal effect against the planktonic form of the yeasts. When using 40 µM of CUR, the metabolic activity of C. albicans, C. glabrata, and C. tropicalis biofilms was reduced by 85%, 85%, and 73%, respectively, at 18 J/cm2. CUR‐mediated PDT also decreased the biofilm biomass of all species evaluated. In addition, CV staining showed that C. albicans isolates were strong biofilm‐forming strains, when compared with C. glabrata and C. tropicalis isolates.ConclusionThe results from the present investigation showed that low CUR concentrations can be highly effective for inactivating Candida isolates when associated with light excitation. Lasers Surg. Med. 43:927‐934, 2011. © 2011 Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 90%

Susceptibility of clinical isolates of Candida to photodynamic effects of curcumin

et al. 2011

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“…This hypothesis can be sustained by the work published by Dahl [15] that investigated the oxidation capability of singlet oxygen on Gram-positive and Gram-negative bacteria. The authors concluded that this extremely reactive free radical presents a very short lifetime (in order of 0,04 µs) and low diffusion capacity.…”

Section: Discussionmentioning

confidence: 90%

Viability study of antimicrobial photodynamic therapy using curcumin, hypericin and photogem photosensitizers in planktonic cells of Streptococcus mutans

Florez

Constante²,

Arco³

et al. 2015

sjod

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Aims: Investigate the potential of curcumin, hypericin and photogem as antimicrobial agents in the antimicrobial photodynamic therapy (APDT) in Streptococcus mutans planktonic cultures. Methods and Results: Susceptibility of S. mutans (ATCC 25175) to APDT using three concentrations of curcumin (250, 750 and 1000 mg l-1), hypericin (1, 5 and 10 mg l-1) and photogem (1, 5 and 10 mg l-1) was examined by colony-forming units method. Separated specimens of each group were irradiated using specific wavelengths (450 ± 10 nm, 590 ± 10 nm and 630 ± 10 nm). Results demonstrate absence of antibacterial effects for the groups that didn't have the association of light with photosensitizers. Antibacterial effects were observed in the groups using photogem and hypericin. When compared with the positive control, the observed reductions were in the order of 6-logs up to the eradication of the treated cells. It is possible to co-relate the increase of photosensitizer concentration and irradiation time with higher antibacterial effect. Conclusions: APDT have potential to be used as an oral disinfectant method. Significance and Impact of the Study: APDT can be used prior to the execution of any oral treatment to decrease the dentist and staff exposure to a big bacterial load during the subsequent treatments and to lower the potential of environmental cross-contamination.

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“…The bactericidal effect of light-irradiated curcumin occurs at relatively short drug-light intervals (0-60 min), whereby longer drug-light intervals (90 min) did not yield an additional cytotoxic effect [95]. These data suggest that curcumin either distributes rapidly throughout the cell or confers its phototoxic effect at superficial loci, from which the generated radical intermediates may induce local damage or diffuse to intracellular targets.…”

Section: Curcuminmentioning

confidence: 86%

“…The photokilling propensity of curcumin was considerably more profound in the Gram-positive S. aureus than the Gramnegative E. coli and Salmonella typhimurium (~300 times more effective when corrected for light dose and concentration) [95], indicating an associative predilection for Gram-positive species. The bactericidal effect of light-irradiated curcumin occurs at relatively short drug-light intervals (0-60 min), whereby longer drug-light intervals (90 min) did not yield an additional cytotoxic effect [95].…”

Section: Curcuminmentioning

confidence: 96%

Antibacterial photodynamic therapy: overview of a promising approach to fight antibiotic-resistant bacterial infections

et al. 2015

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Antibacterial photodynamic therapy (APDT) has drawn increasing attention from the scientific society for its potential to effectively kill multidrug-resistant pathogenic bacteria and for its low tendency to induce drug resistance that bacteria can rapidly develop against traditional antibiotic therapy. The review summarizes the mechanism of action of APDT, the photosensitizers, the barriers to PS localization, the targets, the in vitro-, in vivo-, and clinical evidence, the current developments in terms of treating Gram-positive and Gram-negative bacteria, the limitations, as well as future perspectives. Relevance for patients: A structured overview of all important aspects of APDT is provided in the context of resistant bacterial species. The information presented is relevant and accessible for scientists as well as clinicians, whose joint effort is required to ensure that this technology benefits patients in the post-antibiotic era.

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Photokilling of bacteria by the natural dye curcumin

Cited by 149 publications

References 5 publications

Susceptibility of clinical isolates of Candida to photodynamic effects of curcumin

Susceptibility of clinical isolates of Candida to photodynamic effects of curcumin

Viability study of antimicrobial photodynamic therapy using curcumin, hypericin and photogem photosensitizers in planktonic cells of Streptococcus mutans

Antibacterial photodynamic therapy: overview of a promising approach to fight antibiotic-resistant bacterial infections

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