Photoinduction kinetics of electrical potential in a single chloroplast as studied with micro-electrode technique

Булычев, А. А.; Андрианов, В. К.; Kurella, G. A.; Litvin, F. F.

doi:10.1016/0005-2728(76)90090-6

Cited by 38 publications

(11 citation statements)

References 19 publications

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“…5) might suggest such an inference. In leaves exposed to FRL after preillumination with WL, high photochemical activity of PSI has been shown to be manifested in effective generation of thylakoid electric potential, while the electrogenic effect was low after preillumination with FRL [23]. From comparison of PSI electrogenic activity as a function of WL or FRL preillumination with the respective patterns of ΔA 810 (not shown) it is evident that FRL induced excitation of PSI after preillumination with WL gives rise to effective electro genesis and insignificant changes in P700 redox state.…”

Section: Discussionmentioning

confidence: 99%

Induction kinetics of photosystem I-activated P700 oxidation in plant leaves and their dependence on pre-energization

Булычев

Vredenberg

2010

Russ J Plant Physiol

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Abstract-Absorbance changes ΔA 810 were measured in pea (Pisum sativum L., cv. Premium) leaves to track redox transients of chlorophyll P700 during and after irradiation with far red (FR) light under various preil lumination conditions in the absence and presence of inhibitors and protonophorous uncoupler of photosyn thetic electron transport. It was shown that cyclic electron transport (CET) in chloroplasts of pea leaves oper ates at its highest rate after preillumination of leaves with white light and is strongly suppressed after preillu mination with FR light. The FR light induced suppression was partly released during prolonged dark adaptation. Upon FR illumination of dark adapted leaves, the induction of CET was observed, during which CET activity increased to the peak from the low level and then decreased gradually. The kinetics of P700 oxi dation induced by FR light of various intensities in leaves preilluminated with white light were fit to empirical sigmoid curves containing two variables. In leaves treated with a protonophore FCCP, the amplitude of FR light induced changes ΔA 810 was strongly suppressed, indicating that the rate of CET is controlled by the pH gradient across the thylakoid membrane.

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Section: Discussionmentioning

confidence: 99%

Induction kinetics of photosystem I-activated P700 oxidation in plant leaves and their dependence on pre-energization

Булычев

Vredenberg

2010

Russ J Plant Physiol

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“…The pf18 mutant strain of the algal cells was grown in TAP medium and used for oxygen sensing since it exhibits no motility. Chloroplasts purified from Peperomia metallica leaves were also prepared as described elsewhere (Bulychev et al 1976). …”

Section: Cellsmentioning

confidence: 99%

In vivo O2 measurement inside single photosynthetic cells

et al. 2011

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The oxygen evolution of single cells was investigated using a nano-probe with an ultra-micro electrode (UME) in a submicron sized system in combination with a micro-fluidic system. A single cell was immobilized in the micro-fluidic system and a nano-probe was inserted into the cytosolic space of the single cell. Then, the UME was used for an in vivo amperometric experiment at a fixed potential and electrochemical impedance spectroscopy to detect oxygen evolution of the single cell under various light intensities.

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“…nOX_n k~ k~x +( X+ k2 kl kl+ k2 ) e-(k' + k~)t/n (8) If we define t = 0 as the moment saturating actinic illumination starts and X = 1, i.e. a fully oxidized intermediary redox pool, the two fluxes can be calculated as shown in Figure 2A.…”

Section: The Light Driven Proton Pumpsmentioning

confidence: 99%

“…However, for actinic illuminations of longer duration (~> 10 ms), the absorbance change is obscured by scattering changes and this technique is not applicable [38,12]. The only other kinetic technique available to measure the electric potential changes with sufficient time resolution, during actinic illumination periods of intermediate duration, is the open ended glass capillary microelectrode technique [6][7][8][9][10][11][49][50][51][52]. Recent results, comparing flash-induced potential changes measured with both of the above mentioned techniques in the same specimen, further enhance our trust in the reliability of the microelectrode measurements [47].…”

Section: Introductionmentioning

confidence: 99%

Photosynthetic free energy transduction related to the electric potential changes across the thylakoid membrane

1986

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A model based on our present knowledge of photosynthetic energy transduction is presented. Calculated electric potential profiles are compared with microelectrode recordings of the thylakoid electric potential during and after actinic illumination periods of intermediate duration. The information content of the measured electric response is disclosed by a comparison of experimental results with calculations. The proton flux through the ATP synthase complex is seen to markedly influence the electric response. Also the imbalance in maximum turnover rate between the two photosystems, common to obligate shade plants like Peperomia metallica used in the microelectrode experiments, is clearly reflected in the electric potential profile.

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Photoinduction kinetics of electrical potential in a single chloroplast as studied with micro-electrode technique

Cited by 38 publications

References 19 publications

Induction kinetics of photosystem I-activated P700 oxidation in plant leaves and their dependence on pre-energization

Induction kinetics of photosystem I-activated P700 oxidation in plant leaves and their dependence on pre-energization

In vivo O2 measurement inside single photosynthetic cells

Photosynthetic free energy transduction related to the electric potential changes across the thylakoid membrane

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