Photofunctionalization increases the bioactivity and osteoconductivity of the titanium alloy Ti6Al4V

Minamikawa, Hajime; Ikeda, Takayuki; Att, Wael; Hagiwara, Yoshiyuki; Hirota, Makoto; Tabuchi, Masako; Aita, Hideki; Park, Won-Hee; Ogawa, Takahiro

doi:10.1002/jbm.a.35030

Cited by 66 publications

(68 citation statements)

References 55 publications

(117 reference statements)

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“…Previous studies reported that photofunctionalisation of Ti and Ti6Al4V surfaces enhanced attachment, spread, proliferation, and differentiation of osteoblasts in vitro . 3,13 The present study suggests that photofunctionalisation enhances the bioactivity and osteoconductivity of both surfaces. Notably, the BIC ratios were similar between the UV-treated groups at two weeks and control groups at four weeks, clearly demonstrating an acceleration of osseointegration.…”

Section: Discussionmentioning

confidence: 50%

“…17-19 The results of Ti6Al4V in this study are similar to those in previous reports. 3,5,13 The previous studies indicated that there were no differences in surface morphology before and after UV irradiation. 3,20 This study suggests that the carbon deposition on the Ti and Ti6Al4V surfaces was removed after UV irradiation, increasing the hydroxyl groups and hydrophilicity on both surfaces.…”

Section: Discussionmentioning

confidence: 97%

“…Previous studies showed that the push-in values for photofunctionalised Ti and Ti6Al4V implants were significantly higher than those for control implants. 3,13 Second, only five specimens were included in each group, making it difficult to come to a statistical conclusion. Third, to analyse peri-implant bone regeneration, fluorochrome staining was not performed.…”

Section: Discussionmentioning

confidence: 99%

“…The effect of photofunctionalisation on Ti6Al4V surfaces was demonstrated in vitro and enhanced both bioactivity and osteoconductivity. 13 Photofunctionalised Ti implants are already being used in dentistry, and clinical outcome studies have shown that the use of photofunctionalisation results in a high success rate and decreased healing time. 12 Currently used dental and orthopaedic Ti implants were developed based on the concept of bone–implant integration.…”

Section: Introductionmentioning

confidence: 99%

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Photofunctionalised Ti6Al4V implants enhance early phase osseointegration

Yamauchi

Itabashi²,

Wada

et al. 2017

Bone & Joint Research

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ObjectivesUltraviolet (UV) light-mediated photofunctionalisation is known to improve osseointegration of pure titanium (Ti). However, histological examination of titanium alloy (Ti6Al4V), which is frequently applied in orthopaedic and dental surgery, has not yet been performed. This study examined the osseointegration of photofunctionalised Ti6Al4V implants.MethodsTi and Ti6Al4V implants were treated with UV light, and the chemical composition and contact angle on the surfaces were evaluated to confirm photofunctionalisation. The implants were inserted into femurs in rats, and the rats were killed two or four weeks after the surgery. For histomorphometric analysis, both the bone–implant contact (BIC) ratio and the bone volume (BV) ratio were calculated from histological analysis and microcomputed tomography data.ResultsThe amount of carbon and the contact angle on both implants were significantly reduced after UV irradiation. The BIC ratios for both UV light-treated implants significantly increased at two weeks, but there was no significant difference at four weeks. There was no significant difference in the BV ratios between the UV light-treated and control implants at two or four weeks.ConclusionsThis study suggests that photofunctionalisation of Ti6Al4V implants, similar to that of Ti implants, may promotes osseointegration in early but not in the late phase of osseointegration.Cite this article: R. Yamauchi, T. Itabashi, K. Wada, T. Tanaka, G. Kumagai, Y. Ishibashi. Photofunctionalised Ti6Al4V implants enhance early phase osseointegration. Bone Joint Res 2017;6:331–336. DOI: 10.1302/2046-3758.65.BJR-2016-0221.R1.

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Section: Discussionmentioning

confidence: 50%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Photofunctionalised Ti6Al4V implants enhance early phase osseointegration

Yamauchi

Itabashi²,

Wada

et al. 2017

Bone & Joint Research

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“…We then sterilised them using ethylene oxide (EO) gas at a temperature of 55 °C for 1 h 24, 25 . The prepared titanium discs were fully aged by storing in sealed 12-well cell culture plates under dark ambient conditions at room temperature for at least 8 weeks 26, 27 . After the end of the aging period, we divided the titanium samples into three groups for further processing:

Immediately: The titanium specimens were irradiated by UV under ambient conditions.

…”

Section: Methodsmentioning

confidence: 99%

Overcoming the biological aging of titanium using a wet storage method after ultraviolet treatment

Choi

Jeong

Yul

et al. 2017

Sci Rep

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We evaluated whether the biological activity of the surface of titanium, when stored in an aqueous solution after ultraviolet (UV) treatment, is comparable to that of the surface immediately after UV treatment. We subjected Grade IV titanium discs with machined surfaces to UV radiation for 15 min and then tested them immediately and after storage for 28 days, with and without distilled H 2 O (dH 2 O). We evaluated the surface characteristics using surface profiling, contact angle analysis, X-ray photoelectron spectroscopy, and in terms of the surface zeta-potential. We determined the level of biological activity by analysing albumin adsorption, MC3T3-E1 and human mesenchymal cell adhesion and cytoskeleton development, as well as the production of intracellular reactive oxygen species between groups. The surface characteristics produced by the UV irradiation were maintained in dH 2 O for 28 days. We found that titanium stored in dH 2 O for 28 days after UV treatment exhibited enhanced protein adsorption, cell attachment, and cytoskeleton development. Titanium stored in dH 2 O for 28 days after UV irradiation exhibited a lower level of oxidative stress, comparable to that of the titanium immediately after UV treatment. UV treatment combined with wet storage can be used as a means of overcoming the biological aging of titanium.

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Ultraviolet Light‐Based Micropattern Printing on Titanium Surfaces to Promote Early Osseointegration

Lou

Zhang

Wang

et al. 2023

Adv Healthcare Materials

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Patterned interfaces are widely used for surface modification of biomaterials because of a morphological unit similar to that of native tissue. However, engineering fast and cost‐effective high‐resolution micropatterns directly onto titanium surfaces remains a grand challenge. Herein, a simply designed ultraviolet (UV) light‐based micropattern printing to obtain geometrical patterns on implant interfaces is fabricated by utilizing customized photomasks and titanium dioxide (TiO2) nanorods as a photo‐responsive platform. The technique manipulates the cytoskeleton of micropatterning cells on the surface of TiO2 nanorods. The linear pattern surface shows the elongated morphology and parallel linear arrangements of human mesenchymal stem cells (hMSCs), significantly enhancing their osteogenic differentiation. In addition to the upregulated expression of key osteo‐specific function genes in vitro, the accelerated osseointegration between the implant and the host bone is obtained in vivo. Further investigation indicates that the developed linear pattern surface has an outstanding effect on the cytoskeletal system, and finally activates Yes‐Associated Protein (YAP)‐mediated mechanotransduction pathways, initiating hMSCs osteogenic differentiation. This study not only offers a microfabrication method that can be extended to fabricate various shape‐ and size‐controlled micropatterns on titanium surfaces, but also provides insight into the surface structure design for enhanced bone regeneration.

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Photofunctionalization increases the bioactivity and osteoconductivity of the titanium alloy Ti6Al4V

Cited by 66 publications

References 55 publications

Photofunctionalised Ti6Al4V implants enhance early phase osseointegration

Photofunctionalised Ti6Al4V implants enhance early phase osseointegration

Overcoming the biological aging of titanium using a wet storage method after ultraviolet treatment

Ultraviolet Light‐Based Micropattern Printing on Titanium Surfaces to Promote Early Osseointegration

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