Photoelectron Imaging in Cell Biology

Griffith, O. Hayes; Rempfer, Gertrude F.

doi:10.1146/annurev.bb.14.060185.000553

Cited by 28 publications

(18 citation statements)

References 5 publications

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“…PEEM images for cells (8–10), viruses (11,12) and DNA (11,13–15), among others (16–18), were reported using both labeling and nonlabeling techniques. In addition to the experimental evidence, reviews were written establishing PEEM as a powerful tool for biological imaging (19–21).…”

Section: Introductionmentioning

confidence: 99%

Challenges in Applying Photoemission Electron Microscopy to Biological Systems^†

Peles

Simon²

2009

Photochem & Photobiology

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Photoemission electron microscopy (PEEM) is a unique surface-sensitive instrument capable of providing real-time images with high spatial resolution. While similar to the more common electron microscopies, scanning electron microscopy and transmission electron microscopy, the imaging technology relies on the photogeneration of electrons emitted from a sample through light excitation. This imaging technique has found prominence in surface and materials sciences, being well suited for imaging flat surfaces, and changes that occur to that surface as various parameters are changed (e.g. temperature, exposure to reactive gases). Biologically focused PEEM received significant attention in the 1970s, but was not aggressively advanced since that pioneering work. PEEM is capable of providing important insights into biological systems that extend beyond simple imaging. In this article, we identify and establish important issues that affect the acquisition and analysis of biological samples with PEEM. We will briefly review the biological impact and importance of PEEM with respect to our work. The article also concludes with a discussion of some of the current challenges that must be addressed to enable PEEM to achieve its maximum potential with biological samples.

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Section: Introductionmentioning

confidence: 99%

Challenges in Applying Photoemission Electron Microscopy to Biological Systems^†

Peles

Simon²

2009

Photochem & Photobiology

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“…The support of Rolf Könenkamp, is gratefully acknowledged. and produced the first uncoated images of naked bacterial DNA, and flagellar filaments (Griffith and Rempfer, 1985). The first instrument was returned to Portland State University, the Gertrude F. Rempfer Laboratory upon the retirement of O. Hayes Griffith.…”

Section: Methodsmentioning

confidence: 99%

“…The productive collaboration resulted in several important works, including Griffith and Rempfer (1985); Griffith, 1987;Birrell, Habliston and Griffith, 1994;and Habliston, Hedberg, Birrell, et al, (1995), all key articles in photoemission electron microscopy.…”

Section: Chapter Nine Photoemission Electron Microscopy -Cpemmentioning

confidence: 99%

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Investigations of the Air-Water Interface: A Structural Analysis of Metallic Surface Films and Aquatic Surface Films by Comparative Microscopy

Smith¹

2000

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The air-water interface is an important natural boundary layer that has been neglected as an area of environmental field research. This study establishes that comparative microscopy can be an effective environmental method, and establishes that the term metallic surface films, is a more accurate descriptor than iron oxide surface films. This research shows that surface films are complex, often with layered structure, serve as habitat for significant biota, and act as a point of mineralization to several transition metal elements including manganese, iron, copper, nickel and zinc. This study demonstrates that surface films form under several conditions and can have diverse morphology. Activity of biota, microbes, particularly diatoms, suggests that bacteria and cyanobacteria integrate into the film often in patches, represented by forms and casts. Analytical imaging is used to document and compare film morphology and structures, using scanning electron microscopy, photoemission electron microscopy and transmission electron microscopy with elemental analysis by energy dispersive spectroscopy to confirm the hypothesis. Instrument parameters and strengths are reviewed. Component layers of a copper/zinc film were used to confirm metallic layers and elemental distribution. Bacterial casts were used to confirm film interaction, and to show entrainment and enrichment of the film to incorporate autochthonous and allochthonous materials into the films themselves. Most samples were from Oregon selected sites, with some samples from Maryland and Barbados.ii DEDICATIONThe best way to have good ideas is to have lots of ideas and then develop a method for sorting the good ones from the others.

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“…Later, Griffith and Rempfer performed extensive work in these fields [73], before Tonner and Harp introduced PEEM at synchrotron radiation sources [26]. Using their instrument, de Stasio et al could image neurons using XANES [74].…”

Section: Biomineralsmentioning

confidence: 99%

Chemical and Magnetic Imaging with X-Ray Photoemission Electron Microscopy

Locatelli

Menteş

2014

Synchrotron Radiation

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X-ray photoemission electron microscopy (XPEEM) is a full-field imaging technique giving access to the chemical state and magnetic order of laterally inhomogeneous surfaces, interfaces and thin films. In its simplest variant, synchrotronbased PEEM uses secondary emission to map local differences in the oxidation state, valence, and bond orientation around the emitter. The combination with X-ray circular and linear dichroism techniques represents the most frequent application, and has found extensive use in imaging ferromagnetic and antiferromagnetic domains. XPEEM instruments with energy filter can implement laterally resolved X-ray photoelectron spectroscopy (XPS) and angle resolved photoelectron spectroscopy (ARPES), reaching high chemical and electronic structure sensitivity. Here, we describe the basic aspects and methods of synchrotron-based spectromicroscopy with the PEEM, and its combination with low energy electron microscopy (LEEM). The present state of the art of the technique will be illustrated by applications in diverse fields, spanning from surface and materials sciences to biology and magnetism.

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Photoelectron Imaging in Cell Biology

Cited by 28 publications

References 5 publications

Challenges in Applying Photoemission Electron Microscopy to Biological Systems^†

Challenges in Applying Photoemission Electron Microscopy to Biological Systems^†

Investigations of the Air-Water Interface: A Structural Analysis of Metallic Surface Films and Aquatic Surface Films by Comparative Microscopy

Chemical and Magnetic Imaging with X-Ray Photoemission Electron Microscopy

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Photoelectron Imaging in Cell Biology

Cited by 28 publications

References 5 publications

Challenges in Applying Photoemission Electron Microscopy to Biological Systems†

Challenges in Applying Photoemission Electron Microscopy to Biological Systems†

Investigations of the Air-Water Interface: A Structural Analysis of Metallic Surface Films and Aquatic Surface Films by Comparative Microscopy

Chemical and Magnetic Imaging with X-Ray Photoemission Electron Microscopy

Contact Info

Product

Resources

About

Challenges in Applying Photoemission Electron Microscopy to Biological Systems^†

Challenges in Applying Photoemission Electron Microscopy to Biological Systems^†