Photodynamic therapy and nuclear imaging activities of zinc phthalocyanine‐integrated TiO₂ nanoparticles in breast and cervical tumors

Abstract

In recent years, phthalocyanines (Pcs) have been widely used as photosensitizer in photodynamic therapy applications. Because of their strong absorptions in the near-infrared region (640-700 nm). The integration of phthalocyanine derivatives to a nanoparticle is expected to be efficient way to improve the activity of the photosensitizer on the targeted tissue. It is known that the integrated molecules not only show better accumulation on tumor tissue but also reduce toxicity in healthy tissues. In this study, the ZnPc molecule was synthesized and integrated to the TiO nanoparticle, to investigate the potential of PDT and its cytotoxicity. Additionally, ZnPc and ZnPc-TiO molecules were labeled with I and it was aimed to put forth the nuclear imaging/therapy potentials of I labeled ZnPc/ZnPc-TiO by determining in vitro uptakes in mouse mammary carcinoma (EMT6), human cervical adenocarcinoma (HeLa). In result of our study, it was observed that the radiolabeling yields of the synthesized ZnPc and ZnPc-TiO with I were quite high. In vitro uptake studies shown that I-ZnPc-TiO could be a potential agent for nuclear imaging/treatment of breast and cervical cancers. According to PDT results, ZnPc-TiO might have as to be a potential PDT agent in the treatment of cervical tumor. ZnPc and ZnPc-TiO might be used as theranostic agents.

Titanium Dioxide Nanoparticles – Prospects and Applications in Medicine

“…MCZnPc-TiO2 caused significant phototoxicity in EMT6 cell lines at 1.57 and 3.13 μm with 30 J/cm 2 and 6.25 μm at 60 J/cm 2 . The photocytotoxicity in HeLa cell lines was the highest for MCZnPc-TiO2 at 6.25 μm with 60 J/cm 2 , but MCZnPc was more photocytotoxic at 3.13 μm with 90 J/cm 2 [60].…”

“…Yurt et al used in their study unsymmetrical monocarboxylic derivative of zinc(II) phthalocyanine (MCZnPc) and MCZnPc anchored onto TiO2 NPs -labeled with 131 I to assess their potential in therapy and diagnosis of selected cancers. MCZnPc and MCZnPc-TiO2 NPs were tested for their antitumor activity against mouse mammary carcinoma (EMT6) and human cervical adenocarcinoma (HeLa) cells [60]. After a three hour incubation period in the dark, the cell cultures with photosensitizers were irradiated with light at 684 nm wavelength.…”

Antimicrobial photodynamic therapy against Staphylococcus aureus using zinc phthalocyanine and zinc phthalocyanine-integrated TiO2 nanoparticles

“…It is known that the lipophilicity of Pcs assists them penetrate outer cell walls in bacteria [38,50]. We previously demonstrated that ZnPc is more lipophilic than ZnPc-TiO 2 [37].…”

“…Zinc (II) 9,10,16,17,23,24-hexa(4´-tert-butylphenoxy)-2-[2´-(4´´-carboxyphenyl)ethynyl] phthalocyaninato (2-)-N29, N30, N31,N32 ZnPc are shown in Fig. 2 and ZnPc integrated TiO 2 nanoparticles (ZnPc-TiO 2 ) were synthesized according to the previously reported method [37,38]. Both compounds were dissolved in dimethyl sulfoxide (DMSO) to a final concentration of 64 mg/ml and were stored in the dark at 4 °C before use.…”

“…Also, antimicrobial efficiency of ZnPcs has been tested as in vivo animal models for clinical trials [35,36]. In our previous studies it is observed that synthesized ZnPc has highly promising PDT agent for cancer treatment [23,35,[37][38][39]. For this reason, we have examined APDT efficiency of synthesized ZnPc and ZnPc-TiO 2 in this article.…”