Photodynamic Inactivation of Candida Albicans by Hematoporphyrin Monomethyl Ether

Abstract

Candida albicans can be effectively inactivated by HMME in the presence of light, and HMME-mediated PACT shows its potential as an antifungal treatment.

Evaluation of the photodynamic efficacy and effects of haematoporphyrin monomethyl ether on Trichophyton rubrum microconidia in vitro

“…The TEM samples were prepared according to a previously described method. 28 In brief, microconidia of T rubrum were treated with HMME-mediated aPDT and collected by centrifugation at 3500 rpm for 10 minutes. Next, 2.5% glutaraldehyde was added, and the microconidia were incubated at 4°C for 2 hours.…”

“…[24][25][26] In our previous studies, it was found that HMME-mediated aPDT could inactivate several important gram-positive and gram-negative antibiotic-resistant bacteria and Candida albicans in planktonic condition and biofilms. [27][28][29] However, the photodynamic inactivation (PDI) efficacy and effects of HMME-mediated aPDT on dermatophyte fungi remain unclear. Therefore, in this study, we have assessed the potential of HMME to mediate photodynamic treatment against T rubrum and evaluated its effects on cellular structure in vitro.…”

“…Previous studies demonstrated that photodynamic therapy (PDT) mediated by HMME was effective to kill cancerous cells 24‐26 . In our previous studies, it was found that HMME‐mediated aPDT could inactivate several important gram‐positive and gram‐negative antibiotic‐resistant bacteria and Candida albicans in planktonic condition and biofilms 27‐29 . However, the photodynamic inactivation (PDI) efficacy and effects of HMME‐mediated aPDT on dermatophyte fungi remain unclear.…”

“…(P − L+): microconidia were irradiated with 48 J cm −2 of light. (P + L+): microconidia were incubated with 10 μM HMME in the dark for 2 h and irradiated with 48 J cm −2 of light the results obtained in the HMME-mediated PDI of C albicans 28. In contrast, blue fluorescence from Hoechst 33342 showed no significant difference in its intracellular localisation before and after light irradiation, indicating that HMME-mediated aPDT had inapparent effect on the nucleus, as well as Hoechst 33342 fluorescence was not confined to the nucleus for C albicans after light irradiation.…”

Haematoporphyrin monomethyl ether-mediated photodynamic inactivation of the biofilms produced by standard and fluconazole-resistantCandida albicansstrains

“…Moreover, HMME is less costly than other photoactive drugs . In our previous study, we found that HMME was effective at killing C. albicans cells in planktonic suspensions in the presence of light . The aim of the present work was to assess the PDI efficacy of HMME for the biofilms produced by standard and fluconazole‐resistant C. albicans strains.…”