Photodynamic inactivation of Gram-positive bacteria employing natural resources

Mamone, Leandro; Venosa, Gabriela Di; Gándara, Lautaro; Sáenz, Daniel A.; Vallecorsa, Pablo; Schickinger, Sarah; Rossetti, María Victoria; Batlle, Alcira; Buzzola, Fernanda R.; Casas, Adriana

doi:10.1016/j.jphotobiol.2014.03.003

Cited by 24 publications

(19 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…10 times the singlet oxygen as methylene blue at the concentrations tested (Maraccini et al 2016b). However, rose bengal had a neutral or minimal effect on Gram-negative facultative anaerobes, likely as a result from the added protection provided by the outer membrane that Grampositive bacteria do not have (Dahl et al 1988;Mamone et al 2014). The natural photosensitizers enhanced photoinactivation for 8 of the 10 bacteria-natural photosensitizer pairs tested, although the resultingk exogenous was typically among the lowestk exogenous observed.…”

Section: Discussionmentioning

confidence: 96%

“…; Mamone et al . ). The natural photosensitizers enhanced photoinactivation for 8 of the 10 bacteria‐natural photosensitizer pairs tested, although the resulting

{\overset{false^}{k}}_{exogenous}

was typically among the lowest

{\overset{false^}{k}}_{exogenous}

observed.…”

Section: Discussionmentioning

confidence: 97%

“…; Mamone et al . ) that the Gram‐positive bacteria do not have. To our knowledge, this is the first study to report the vulnerability of Gram‐negative bacteria to exogenous‐induced photoinactivation.…”

Section: Discussionmentioning

confidence: 99%

“…In Gram-negative bacteria, the additional outer membrane may confer some protection against exogenous photoinactivation (Dahl et al 1988;Mamone et al 2014) that the Gram-positive bacteria do not have. To our knowledge, this is the first study to report the vulnerability of Gram-negative bacteria to exogenousinduced photoinactivation.…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Exogenous indirect photoinactivation of bacterial pathogens and indicators in water with natural and synthetic photosensitizers in simulated sunlight with reduced UVB

2016

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 96%

“…; Mamone et al . ). The natural photosensitizers enhanced photoinactivation for 8 of the 10 bacteria‐natural photosensitizer pairs tested, although the resulting

{\overset{false^}{k}}_{exogenous}

was typically among the lowest

{\overset{false^}{k}}_{exogenous}

observed.…”

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Exogenous indirect photoinactivation of bacterial pathogens and indicators in water with natural and synthetic photosensitizers in simulated sunlight with reduced UVB

2016

View full text Add to dashboard Cite

show abstract

“…While photodynamic therapy was, for example, established as a successful modality for malignancies and age‐related macular degeneration , PDI has only recently been intensively investigated as an alternative route for the eradication of microorganisms . MB is a cationic phenothiazine dye binding to chemical groups with a negative charge, an ability to enter into oxidation‐reduction processes, and a high photoactivity.…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of the Photoactive Dye Methylene Blue versus Caspofungin on the Candida parapsilosis Biofilm in vitro and ex vivo

Černáková

Chupáčová

Židlíková

et al. 2015

Photochem & Photobiology

View full text Add to dashboard Cite

This research studied the effectiveness of the photoactive compound methylene blue (MB) activated with red LED light (576-672 nm) compared to that of caspofungin (CAS) on 1 Candida albicans and 3 Candida parapsilosis strains. Results were evaluated in terms of SMIC50 for CAS or in PDI (photodynamic inactivation)-SMIC50 for MB (minimal inhibitory concentration inhibiting sessile biofilm to 50% in comparison to the control without CAS or after irradiation in comparison to the control without MB). While all strains were susceptible to CAS in planktonic form, the SMIC50 was determined to be >16 μg mL(-1) when CAS was added to a 24 h biofilm. However, PDI-MIC50s (1.67 mW cm(-2) , fluence 15 J cm(-2) ) were 0.0075-0.03 mmol L(-1) . For biofilm, PDI-SMIC50s were in the range from 0.7 to 1.35 mmol L(-1) . MB concentration of 1 mmol L(-1) prevented a biofilm being formed ex vivo on mouse tongues after irradiation regardless of the application time, in contrast to CAS, which was only effective at a concentration of 16 μg mL(-1) when it was added at the beginning of biofilm formation. PDI seems to be a promising method for the prevention of microbial biofilms that do not respond significantly to conventional drugs.

show abstract

Antimicrobial and Light‐Enhanced Antimicrobial Activities, Cytotoxicity and Chemical Variability of All Tunisian Eryngium Species

Landoulsi

Hennebelle

Béro

et al. 2020

Chemistry & Biodiversity

View full text Add to dashboard Cite

This study was performed on all Eryngium species growing in Tunisia in order to evaluate their intra and interspecies variabilities and to investigate their biological activities. These species are used in traditional medicine, and literature about the phytochemical investigations of most of them is scarce. Antimicrobial and light-enhanced activities were tested against multiresistant microorganisms and extended spectrum betalactamase producing bacteria (ESBL). All studied species showed antimicrobial effect with several MIC values lower than 70 μg/ml. Tested Eryngium species have proven to be a promising source of photoactive compounds, while light-enhanced activity offers an alternative for the inactivation of pathogenic microorganisms which is currently subjected to a great interest. This is the first report of this activity in genus Eryngium. A significant improvement of antimicrobial activity with UV irradiation was observed, mainly for E. dichotomum, E. ilicifolium and E. triquetrum. Cytotoxicity, studied for the first time for the most species, was evaluated against cancer (J774) and non-cancer (WI38) human cell lines. Chemical composition of volatile compounds presented in the most active crude extracts (petroleum ether extracts) of the aerial parts was investigated using GC/MS analysis and was submitted to statistical analyses. It revealed their high content of bioactive phytochemicals, particularly oxygenated sesquiterpenes like spathulenol, ledol and α-bisabolol but also hydrocarbon sesquiterpenes such as β-bisabolene and copaene, as well as polyacetylene derivatives such as falcarinol. Statistical analyses permitted to evaluate the interrelations between all Tunisian Eryngium species. Figure 3. Principal component analysis of Tunisian Eryngium species.

show abstract

Photodynamic inactivation of Gram-positive bacteria employing natural resources

Cited by 24 publications

References 52 publications

Exogenous indirect photoinactivation of bacterial pathogens and indicators in water with natural and synthetic photosensitizers in simulated sunlight with reduced UVB

Exogenous indirect photoinactivation of bacterial pathogens and indicators in water with natural and synthetic photosensitizers in simulated sunlight with reduced UVB

Effectiveness of the Photoactive Dye Methylene Blue versus Caspofungin on the Candida parapsilosis Biofilm in vitro and ex vivo

Antimicrobial and Light‐Enhanced Antimicrobial Activities, Cytotoxicity and Chemical Variability of All Tunisian Eryngium Species

Contact Info

Product

Resources

About