Photodynamic effects on human and chicken erythrocytes studied with microirradiation and confocal laser scanning microscopy

König, Karsten; Kimel, S.; Berns, Michael W.

doi:10.1002/(sici)1096-9101(1996)19:3<284::aid-lsm4>3.0.co;2-o

Cited by 9 publications

(5 citation statements)

References 18 publications

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“…For a number of phototoxic agents, the plasma membrane is the target of phototoxicity (16)(17)(18)(19)(20)(21). However, for BCA the situation is apparently different.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Photodynamic Therapy on Human Neutrophil Migration Using Bacteriochlorin a

Schuitmaker

Koster

Elferink

1998

Photochem & Photobiology

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Bacteriochlorin a photodynamic therapy (BCA-PDT) caused inhibition of interleukin (IL)-8-activated neutrophil migration, at concentrations that did not induce membrane damage. Random migration and migration induced by other chemoattractants were also inhibited, indicating that the effect of BCA-PDT was not at the level of chemoattractant-receptor interaction but down stream. The BCA-PDT completely blocked superoxide production of phorbol ester-stimulated neutrophils indicating that superoxide production by neutrophils present in the tumor before and during BCA-PDT is not the cause of inactivation of tumor cells. Both type I and type II quenchers prevented inhibition by BCA-PDT but only in electroporated cells. Confocal laser scanning microscopy showed that the fluorescence of BCA was located inside the cell. These results show that the effects of BCA-PDT are intracellular and of a mixed type I/type II character and that the neutrophils present in the tumor during illumination probably do not contribute to tumor eradication by releasing reactive oxygen species.

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“…For a number of phototoxic agents, the plasma membrane is the target of phototoxicity (16)(17)(18)(19)(20)(21). However, for BCA the situation is apparently different.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Photodynamic Therapy on Human Neutrophil Migration Using Bacteriochlorin a

Schuitmaker

Koster

Elferink

1998

Photochem & Photobiology

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“…This is the case for avian erythrocytes, 121 which can be conveniently used for relevant PDT studies. The first investigation on photodynamic effects on cell organelles of chicken erythrocytes has been published, 122 although it did not focus on mitochondria.…”

Section: Future Aspectsmentioning

confidence: 99%

Erythrocytes—the ‘house elves’ of photodynamic therapy

Kaestner

Juzeniene

Moan

2004

Photochem Photobiol Sci

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Photodynamic therapy (PDT) and fluorescence diagnosis (FD) are being developed for a number of clinical applications. Since fluorophores and photosensitising drugs are usually given systemically their effect on blood elements are of significant importance. Photodynamic effects on erythrocytes occur naturally in patients with erythropoietic protoporphyria (EPP). Exposure to small fluences, as obtained by the erythrocytes when they pass capillaries in the skin, leads to transfer of the photosensitiser protoporphyrin IX (PP IX), from EPP erythrocytes to endothelial cells. Thus, the erythrocytes are partly protected while the endothelial cells suffer photodamage. During photodynamic therapy in vivo erythrocytes are regularly photosensitised. This side effect is partly intended but mostly unwanted, and a summary of this topic is given. Furthermore, the effect of UV-A on erythrocytes that is accompanied with the formation of bilirubin is reviewed. Erythrocytes serve as convenient model cells for experimental research. Such use of erythrocytes to screen new photosensitisers may be of limited value. A combination of photohaemolysis and haemoglobin oxygenation may become the basis for an assay for in vitro phototoxicity. Erythrocytes from birds are good model cells for exploration of physiological and molecular mechanisms involved in PDT. A potential mechanism of PDT induced behaviour resembling apoptosis in erythrocytes is provided.PDT for sterilisation of erythrocyte concentrates has a potential for medical use. Photodynamic effects on the erythrocytes themselves should be avoided. This is realised by choosing a virus-selective photosensitiser, low fluences and treatment of the concentrates with agents like dipyridamole and antioxidants. Future aspects of applications of photosensitisation of red blood cells are discussed.

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“…This rapidly leads to vasoconstriction and cell death via both necrotic and apoptotic pathways (47)(48)(49). BPD-mediated PDT, in particular, induces severe vasoconstriction, which has been directly observed using light microscopy (50,51) and optical coherence tomography (52). Evidence for the formation of reactive-oxygen intermediates has been shown to be coupled with the depletion of ground-state molecular oxygen (5).…”

Section: Discussionmentioning

confidence: 99%

Monitoring Tumor Response During Photodynamic Therapy Using Near-infrared Photon-migration Spectroscopy¶

Pham¹,

Hornung²,

Berns³

et al. 2007

Photochemistry and Photobiology

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Benzoporphyrin-derivative (BPD)-monoacid-ring A photodynamic therapy (PDT) was performed on subcutaneous tumor implants in a rat ovarian cancer model. In order to assess PDT efficacy the tumor and normal tissue optical properties were measured noninvasively prior to and during PDT using frequency-domain photon migration (FDPM). FDPM data were used to quantify tissue absorption and reduced scattering properties (given by the parameters a and s , respectively) at four nearinfrared (NIR) wavelengths (674, 811, 849 and 956 nm). Tissue physiologic properties, including the in vivo concentration of BPD, deoxy-hemoglobin (Hb), oxy-hemoglobin (HbO 2 ), total hemoglobin (TotHb), water (H 2 O) and percent tissue hemoglobin oxygen saturation (%S t O 2 ), were calculated from optical property data. PDT efficacy was also determined from morphometric analysis of tumor necrosis in histologic specimens. All the measured tumor properties changed significantly during PDT. [Hb] increased by 9%, while [HbO 2 ], [TotHb] and %S t O 2 decreased by 18, 7 and 12%, respectively. Using histologic data we show that long-term PDT efficacy is highly correlated to mean BPD concentration in tumor and PDT-induced acute changes in [HbO 2 ], [TotHb] and %S t O 2 (correlation coefficients of 0.829, 0.817 and 0.953, respectively). Overall, our results indicate that NIR FDPM spectroscopy is able to quantify noninvasively and dynamically the PDT-induced physiological effects in vivo that are highly correlated with therapeutic efficacy. tration in tumor during PDT

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Photodynamic effects on human and chicken erythrocytes studied with microirradiation and confocal laser scanning microscopy

Cited by 9 publications

References 18 publications

The Effects of Photodynamic Therapy on Human Neutrophil Migration Using Bacteriochlorin a

The Effects of Photodynamic Therapy on Human Neutrophil Migration Using Bacteriochlorin a

Erythrocytes—the ‘house elves’ of photodynamic therapy

Monitoring Tumor Response During Photodynamic Therapy Using Near-infrared Photon-migration Spectroscopy¶

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