The Effects of Photodynamic Therapy on Human Neutrophil Migration Using Bacteriochlorin a

Schuitmaker, J. J.; Koster, Ben M. de; Elferink, J. G. R.

doi:10.1111/j.1751-1097.1998.tb05293.x

Cited by 13 publications

(14 citation statements)

References 17 publications

(5 reference statements)

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“…In the case of tumors, the response to PDT is thought to be caused by a combination of direct cytotoxicity and vascular occlusion, which leads to tumor ischemia (36). Inflammatory reactions are also very important, because they give rise to neutrophil infiltration, which itself participates in tumor cell eradication (30). In these last two situations, endothelial cells play a pivotal role.…”

Section: Discussionmentioning

confidence: 99%

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Involvement of Oxidative Stress in NF-κB Activation in Endothelial Cells Treated by Photodynamic Therapy¶

Volanti¹,

Matroule²,

Piette³

2002

Photochem Photobiol

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In human endothelial cells ECV 304 and HMEC-1 photosensitized by pyropheophorbide-a methylester (PPME) in sublethal conditions transcription factor Nuclear Factor kappa B (NF-kappaB) activation takes place for several hours. Activated NF-kappaB was functional because it stimulated the transcriptional activation of either a transfected reporter gene or the endogenous gene encoding interleukin (IL)-8. Concomitant with NF-kappaB activation, inhibitor of NF-kappaB alpha (IkappaB alpha) was degraded during photosensitization and IkappaB beta, p100, p105 and IkappaB epsilon were slightly modified. Reactive oxygen species (ROS) were shown to be crucial intermediates in the activation because antioxidants strongly decreased NF-kappaB activation. Using both a fluorescent probe and isotope substitution, it was shown that ROS, and especially singlet oxygen (1O2), were important in the activation process. Because NF-kappaB activation in the presence of ROS was suspected to proceed through a pathway independent of the IkappaB kinases (IKK), we demonstrated that the IKK were indeed not activated by photosensitization but required an intact tyrosine residue at position 42 on IkappaB alpha, suggesting the involvement of a tyrosine kinase in the activation process. This was further reinforced by the demonstration that herbimycin A, a tyrosine kinase inhibitor, prevented NF-kappaB activation by photosensitization but not by TNF alpha, a cytokine known to activate NF-kappaB through an IKK-dependent mechanism.

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Section: Discussionmentioning

confidence: 99%

“…genes encoding chemokines. These genes were chosen because neutrophil recruitment was reported post-PDT (30). As shown in Fig.…”

Section: Nf-b Is Activated In Endothelial and Colon Cancer Cells By Pmentioning

confidence: 99%

Involvement of Oxidative Stress in NF-κB Activation in Endothelial Cells Treated by Photodynamic Therapy¶

Volanti¹,

Matroule²,

Piette³

2002

Photochem Photobiol

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“…BCA can be an effective PDT that induces death of cancer cells; it has also been applied for locating tumor tissues both in vitro and in vivo . Therefore, we attempted to determine a specific enzyme that can obtain BChlide a, which could be produced directly by degrading BChl a. BChl a is similar to Chls that can perform a unique light‐capturing errand for photosynthesis in photosynthetic bacteria .…”

Section: Discussionmentioning

confidence: 99%

“…However, the physical and chemical properties of Photofrin II™ restrict its applicability; thus, current studies have focused on developing new photosensitizers. Previous studies have reported that bacteriochlorin a (BCA) is a favorable photosensitizer candidate for both in vitro and in vivo studies, and rarely causes allergic reactions or photosensitizes the skin . WST09 and WST11 are used in clinical evaluations of commercial BCA.…”

Section: Introductionmentioning

confidence: 99%

A novel recombinant chlorophyllase from cyanobacterium Cyanothece sp. ATCC 51142 for the production of bacteriochlorophyllide a

Chou

Lee

Yen

et al. 2015

Biotech and App Biochem

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Bacteriopheophorbide a (BPheid a) is used as a precursor for bacteriochlorin a (BCA), which can be used for photodynamic therapy in both in vitro and in vivo biochemical applications. This study successfully isolated and expressed a photosynthetic bacterium (Cyanothece sp. ATCC 51142) chlorophyllase called CyanoCLH, which can be used as a biocatalyst for the production of a BCA precursor by degrading bacteriochlorophyll a (BChl a). Substrate specificity and enzyme kinetic analyses were performed and the results verified that the recombinant CyanoCLH preferred hydrolyzing BChl a to produce bacteriochlorophyllide a (BChlide a), which can be converted to BPheid a by removing magnesium ion. The recombinant CyanoCLH was cloned and expressed in Escherichia coli BL-21 (DE3), and its molecular weight was 54.7 kDa. The deduced amino acid sequence of the recombinant CyanoCLH comprised a unique lipase-motif GHSLG, which differs from the GHSRG sequence of other plants and lacks a histidine of the typical and conserved catalytic triad Ser-Asp-His. The recombinant CyanoCLH was subjected to biochemical analyses, and the results indicated that its optimal pH and temperature were 7.0 and 60 °C, respectively.

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“…Two BCs recently investigated consist of a BChla-serine complex 8 and a BChl a derivative, BCa, 9 which absorb light in the 760-780 nm region. A considerable effort is now devoted to additional secondgeneration photosensitizers with tailored light-absorption properties such as the purpurins, porphyrins with annelated cyclopentenyl rings which are formally at the saturated level of chlorins and typically absorb light in the 650-715 nm region.…”

Section: Introductionmentioning

confidence: 99%

Magnetic Resonance Studies and Molecular Orbital Calculations on the Doublet and Triplet States of Bacteriopurpurin: a Potential Second-Generation Photosensitizer for Photodynamic Therapy

Kay¹,

Conti²,

Fuhs³

et al. 2002

J. Phys. Chem. B

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Bacteriopurpurin is a macrocyclic compound at the saturation level of bacteriochlorins that was specifically synthesized as a potential photosensitizer in photodynamic therapy. It exhibits a long-wavelength Q y absorption band at 843 nm, which is a necessary prerequisite for a deep tissue penetration. Magnetic resonance spectroscopic properties of bacteriopurpurin have been examined by fluorescence-detected magnetic resonance (FDMR) and electron paramagnetic resonance (EPR) in conjunction with molecular orbital calculations. The electronic structure of the radical cation state obtained by oxidation of bacteriopurpurin with iodine is mapped by the determination of isotropic proton hyperfine coupling constants from electron-nuclear double-resonance spectroscopy at 298 K. A tentative assignment of the couplings to the various proton positions in the molecule is achieved by comparison of the experimental values with simulated ones based on semiempirical intermediate neglect of differential overlap calculations and density functional theory. The triplet-state electronic structure of the ground-state trans-configured bacteriopurpurin is characterized by the determination of the zero-field splitting parameters which represent sensitive probes for the geometric structure of the molecule. The |D| and |E| values obtained from time-resolved EPR (24.4 and 5.4 mT) are in favorable agreement with the respective values obtained from FDMR (24.0 and 5.3 mT). By both techniques, the presence of additional photoexcited triplet states has been confirmed. It is assumed that they arise from higher-energy tautomers of bacteriopurpurin. Their occurrence is discussed in terms of a photoinitiated intramolecular one-proton transfer from either ring II or ring IV of the trans-configured ground state to ring I or ring III to form one of the cis-configured bacteriopurpurins.

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The Effects of Photodynamic Therapy on Human Neutrophil Migration Using Bacteriochlorin a

Cited by 13 publications

References 17 publications

Involvement of Oxidative Stress in NF-κB Activation in Endothelial Cells Treated by Photodynamic Therapy¶

Involvement of Oxidative Stress in NF-κB Activation in Endothelial Cells Treated by Photodynamic Therapy¶

A novel recombinant chlorophyllase from cyanobacterium Cyanothece sp. ATCC 51142 for the production of bacteriochlorophyllide a

Magnetic Resonance Studies and Molecular Orbital Calculations on the Doublet and Triplet States of Bacteriopurpurin: a Potential Second-Generation Photosensitizer for Photodynamic Therapy

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