Photodynamic effectiveness of laser diode combined with ozone to reduce STaphylicoccus aureus biofilm with exogenous chlorophyll of Dracaena angustifolia leaves

Astuti, Suryani Dyah; Drantantiyas, Nike Dwi Grevika; Puspita, Putri Suryaning; Syahrom, Ardiyansyah; Suhariningsih, S.

doi:10.24931/2413-9432-2019-8-2-4-13

Cited by 20 publications

(14 citation statements)

References 23 publications

(1 reference statement)

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“…Biofilm research is also found in other journals. 22,23 The difference in the reduction rate of PDT increased when ozone was added, thus helping biofilm reduction compared to PDT. 22 The results showed that the Chlo+Ozon+Laser combination treatment at 20 seconds of ozone exposure with 4 minutes irradiation time caused a decrease in biofilm activity by 80.26%, which was the highest efficacy of all treatment groups.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the antimicrobial reduction effect of photodynamic inactivation with the addition of chlorophyll and curcumin photosensitizer in Aggregatibacter actinomycetemcomitans and Enterococcus faecalis

Arifianto,

Astuti,

Medyaz

et al. 2024

F1000Res

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Background Aggregatibacter actinomycetemcomitans and Enterococcus faecalis are pathogenic bacteria of the oral cavity that cause various diseases such as periodontitis and endodontics. These bacteria are easily resistant to antibiotics. Photodynamic inactivation (PDI) is a method of inactivating microorganisms that utilizes light to activate a photosensitizer agent (PS) that produces reactive oxygen species causing cell lysis. Methods This study used the PDI method with a 405 nm diode laser at various energy density with the addition PS curcumin or chlorophyll Alfalfa, as much as 1.6 mg/ml on A. actinomycetemcomitans and E. faecalis bacteria. Results The study on E. faecalis bacteria showed that the energy density diode laser irradiation of 1.59 J/cm² gave the percentage of E. faecalis bacteria death 36.7% without PS, 69.30% with the addition of chlorophyll Medicago sativa L and 89.42% with the addition of curcumin. Meanwhile, the bacteria A. actinomycetemcomitans showed that the energy density diode laser irradiation of 1.59 J/cm² gave the percentage of bacterial death 35.81% without PS, 64.39% with the addition of chlorophyll Medicago sativa L and 89.82% with the addition of curcumin. PS was critical to the success of the PDI. Conclusions The addition of PS curcumin increased the effectiveness of reducing bacteria E. faecalis and A. actinomycetemcomitans compared to chlorophyll Medicago sativa L.

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Section: Discussionmentioning

confidence: 99%

Comparison of the antimicrobial reduction effect of photodynamic inactivation with the addition of chlorophyll and curcumin photosensitizer in Aggregatibacter actinomycetemcomitans and Enterococcus faecalis

Arifianto,

Astuti,

Medyaz

et al. 2024

F1000Res

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“…Усиление антибактериального эффекта в культуре золотистого стафилококка было отмечено группой авторов при использовании люминофора хлорофилла за счет переизлучения в красную область видимого спектра [14].…”

Section: оригинальные статьи Original Articlesunclassified

Application of visible longwave radiation for inactivation of microorganisms

Kasparova¹,

Yang²,

Bocharova³

et al. 2020

Vestn. oftal'mol.

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РЕЗЮМЕ Цель исследования. Сравнительная оценка бактерицидного и фунгицидного воздействия разных частей спектра излучения (ультрафиолетовых лучей спектра А (УФА), красного, зеленого и синего). Материал и методы. В исследование были включены штаммы наиболее клинически значимых микроорганизмов-S. aureus, P. aeruginosa и грибы C. albicans. После равномерного заселения поверхности чашек Петри микроорганизмами каждой культуры, на 4 из 5 образцов центральную зону поверхности диаметром 1 см облучали разными спектрами света-от ультрафиолетового до красного при одинаковой общей плотности энергии облучения 5,4 Дж/см 2. Один образец оставался контрольным. После облучения с помощью сканирующей электронной микроскопии с лантаноидным контрастированием (СЭМЛК) оценивали общую метаболическую активность, активность эффлюкс-систем и морфологические характеристики микроорганизмов. Результаты. В эксперименте доказано повреждающее действие светового и УФА-излучений на клетки микроорганизмов при исследовании методом СЭМЛК облученных разными частями спектра видимого диапазона и УФА-излучением культур S. aureus, P. aeruginosa и C. albicans. Наибольшей антимикробной активностью обладало излучение зеленого спектра с длиной волны 500 нм. Это проявлялось снижением общего уровня метаболической активности (с 40-63 до 26-37 усл. ед. (S. aureus (p<0,01), P. aeruginosa (p<0,01) и C. albicans (p<0,05)), а также ростом доли клеток S. aureus с активными эффлюкссистемами в 2 раза. Заключение. Метод СЭМЛК позволяет оценивать жизнеспособность и функциональное состояние клеток микроорганизмов на основании определения их морфологических характеристик (форма и размеры) и функциональных показателей (общая метаболическая активность, активация эффлюкс-систем). При исследовании облученных культур S. aureus, P. aeruginosa и C. albicans методом СЭМЛК показано повреждающее излучение зеленого спектра с длиной волны 500 нм. Этот факт может послужить основой для дальнейших исследований и разработки метода лечения инфекционных кератитов с применением зеленого света. Ключевые слова: спектр излучения, ультрафиолетовые лучи спектра А, красный спектр излучения, зеленый спектр излучения, синий спектр излучения, сканирующая электронная микроскопия, бактерицидное и фунгицидное действие, инфекционные кератиты.

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“…Chlorophyll is a green substance found in green plants that photosynthesizes. 13 In photosynthesis, chlorophyll acts as a light catcher, energy transfer, and light conversion and can absorb a maximum wavelength between 400-700 nm. 14 Curcumin is a curcuminoid compound with yellow pigment in turmeric rhizome, which is antitumor, antioxidant, anticarcinogenic, anti-inflammatory, antiviral, antifungal, antispasmodic, and hepatoprotective.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the antimicrobial reduction effect of photodynamic inactivation with the addition of chlorophyll and curcumin photosensitizer in Aggregatibacter actinomycetemcomitans and Enterococcus faecalis

et al. 2023

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Background: Aggregatibacter actinomycetemcomitans and Enterococcus faecalis are pathogenic bacteria of the oral cavity that cause various diseases such as periodontitis and endodontics. These bacteria are easily resistant to antibiotics. Photodynamic inactivation (PDI) is a method of inactivating microorganisms that utilizes light to activate a photosensitizer agent (PS) that produces reactive oxygen species causing cell lysis. Methods: This study used the PDI method with a 405 nm diode laser at various energy density with the addition PS curcumin or chlorophyll Alfalfa, as much as 1.6 mg/ml on A. actinomycetemcomitans and E. faecalis bacteria. Results: The study on E. faecalis bacteria showed that the energy density diode laser irradiation of 1.59 J/cm² gave the percentage of E. faecalis bacteria death 36.7% without PS, 69.30% with the addition of chlorophyll Medicago sativa L and 89.42% with the addition of curcumin. Meanwhile, the bacteria A. actinomycetemcomitans showed that the energy density diode laser irradiation of 1.59 J/cm² gave the percentage of bacterial death 35.81% without PS, 64.39% with the addition of chlorophyll Medicago sativa L and 89.82% with the addition of curcumin. PS was critical to the success of the PDI. Conclusions: The addition of PS curcumin increased the effectiveness of reducing bacteria E. faecalis and A. actinomycetemcomitans compared to chlorophyll Medicago sativa L.

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Photodynamic effectiveness of laser diode combined with ozone to reduce STaphylicoccus aureus biofilm with exogenous chlorophyll of Dracaena angustifolia leaves

Cited by 20 publications

References 23 publications

Comparison of the antimicrobial reduction effect of photodynamic inactivation with the addition of chlorophyll and curcumin photosensitizer in Aggregatibacter actinomycetemcomitans and Enterococcus faecalis

Comparison of the antimicrobial reduction effect of photodynamic inactivation with the addition of chlorophyll and curcumin photosensitizer in Aggregatibacter actinomycetemcomitans and Enterococcus faecalis

Application of visible longwave radiation for inactivation of microorganisms

Comparison of the antimicrobial reduction effect of photodynamic inactivation with the addition of chlorophyll and curcumin photosensitizer in Aggregatibacter actinomycetemcomitans and Enterococcus faecalis

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