Photodynamic effect of TPP encapsulated in polystyrene nanoparticles toward multi-resistant pathogenic bacterial strains: AFM evaluation

Malá, Zuzana; Žárská, Ludmila; Malina, Lukáš; Langová, Kateřina; Večeřová, Renata; Kolář, Milan; Henke, Petr; Mosinger, Jiří; Kolářová, Hana

doi:10.1038/s41598-021-85828-9

Cited by 9 publications

(6 citation statements)

References 46 publications

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“…In addition to the role of nanovehicle, the polymeric particles may also help to address the lack of solubility in the biological medium from the PSs and/or reduce their toxicity, as reported among others by Gualdesi et al [320,322]. For instance, polystyrene NPs with encapsulated hydrophobic TPP-NP(5,10,15,20-tetraphenylporphyrin) have been reported as nano-PS for efficient aPDI approaches towards multi-resistant bacteria [321]. In addition, biocompatible PLGA (polylactic-co-glycolic acid) NPs were employed to encapsulate curcumin, which could potentially serve as an orthodontic adhesive antimicrobial additive [322].…”

Section: Polymeric Nps and Nanocompositesmentioning

confidence: 95%

“…In aPDT, polymeric NPs may be either used to encapsulate PS molecules (loading with PSs), or built from inherent photoactive polymers acting as PSs [303]. Various PS molecules (e.g., RB, porphyrin derivatives, or curcumin) encapsulated in biocompatible polymeric NPs, such as polystyrene-, PEG-, polyester-(including poly-beta-amino esters), or polyacrylamide-based NPs, are widely used in aPDT/PDI approaches, as recently reported [319][320][321]. In addition to the role of nanovehicle, the polymeric particles may also help to address the lack of solubility in the biological medium from the PSs and/or reduce their toxicity, as reported among others by Gualdesi et al [320,322].…”

Section: Polymeric Nps and Nanocompositesmentioning

confidence: 99%

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Antimicrobial Photodynamic Therapy: Latest Developments with a Focus on Combinatory Strategies

et al. 2021

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Antimicrobial photodynamic therapy (aPDT) has become a fundamental tool in modern therapeutics, notably due to the expanding versatility of photosensitizers (PSs) and the numerous possibilities to combine aPDT with other antimicrobial treatments to combat localized infections. After revisiting the basic principles of aPDT, this review first highlights the current state of the art of curative or preventive aPDT applications with relevant clinical trials. In addition, the most recent developments in photochemistry and photophysics as well as advanced carrier systems in the context of aPDT are provided, with a focus on the latest generations of efficient and versatile PSs and the progress towards hybrid-multicomponent systems. In particular, deeper insight into combinatory aPDT approaches is afforded, involving non-radiative or other light-based modalities. Selected aPDT perspectives are outlined, pointing out new strategies to target and treat microorganisms. Finally, the review works out the evolution of the conceptually simple PDT methodology towards a much more sophisticated, integrated, and innovative technology as an important element of potent antimicrobial strategies.

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Section: Polymeric Nps and Nanocompositesmentioning

confidence: 95%

Section: Polymeric Nps and Nanocompositesmentioning

confidence: 99%

Antimicrobial Photodynamic Therapy: Latest Developments with a Focus on Combinatory Strategies

et al. 2021

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“…In this in vitro study, aqueous dispersion of highly sulfonated polystyrene NP with embedded 5,10,15,20-tetraphenylporphyrin (TPP) ( Figure 1 ) was tested. The photodynamic effect of TPP-NP in the presence of bacterial strains was already observed in our previous study [ 26 ]; based on these results, the testing of TPP embedded NP was extended to the non-tumor NIH3T3 cell line and tumor cell lines HeLa and G361 . Cell viability, ROS generation, and changes in mitochondrial membrane potential were studied.…”

Section: Introductionmentioning

confidence: 61%

Biological Evaluation of Photodynamic Effect Mediated by Nanoparticles with Embedded Porphyrin Photosensitizer

Žárská

Malá

Langová

et al. 2022

IJMS

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Clinically approved photodynamic therapy (PDT) is a minimally invasive treatment procedure that uses three key components: photosensitization, a light source, and tissue oxygen. However, the photodynamic effect is limited by both the photophysical properties of photosensitizers as well as their low selectivity, leading to damage to adjacent normal tissue and/or inadequate biodistribution. Nanoparticles (NPs) represent a new option for PDT that can overcome most of the limitations of conventional photosensitizers and can also promote photosensitizer accumulation in target cells through enhanced permeation and retention effects. In this in vitro study, the photodynamic effect of TPP photosensitizers embedded in polystyrene nanoparticles was observed on the non-tumor NIH3T3 cell line and HeLa and G361 tumor cell lines. The efficacy was evaluated by viability assay, while reactive oxygen species production, changes in membrane mitochondrial potential, and morphological changes before and after treatment were imaged by atomic force microscopy. The tested nanoparticles with embedded TPP were found to become cytotoxic only after activation by blue light (414 nm) due to the production of reactive oxygen species. The photodynamic effect observed in this evaluation was significantly higher in both tumor lines than the effect observed in the non-tumor line, and the resulting phototoxicity depended on the concentration of photosensitizer and irradiation time.

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“…Due to their ability to interact with light and rate, these molecules can be photosensitizers in aPDT. Several fi inactivated using porphyrins as PSs in aPDT [15][16][17]35,[52][53][54][55][56][57][58] porphyrins are more active as PSs against Gram-positive and Gra anionic or non-ionic PSs. Work by Banfi et al showed that posi porphyrins are important for PS antibacterial activity [80], as the into the cell.…”

Section: Use Of Porphyrins As Pss Against Fish Bacterial Pathogensmentioning

confidence: 99%

Applications of Antimicrobial Photodynamic Therapy in Aquaculture: Effect on Fish Pathogenic Bacteria

Dube,

Okuthe

2024

Fishes

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Increased infectious diseases and the reduced effectiveness of antibiotics due to antimicrobial resistance pose global challenges affecting the aquaculture industry. As bacteria increasingly develop antibiotic resistance, research scientists are shifting their focus to technologies such as antimicrobial photodynamic therapy (aPDT), which show potential for treating and controlling fish infections without promoting the development of resistant bacteria. Various photosensitizers (PSs), both natural and synthetic, are under investigation for their application in aPDT within the aquaculture industry. This shift is crucial for the sustainability of the aquaculture industry, which plays a significant role in achieving several of the United Nations (UN) Sustainable Development Goals (SDGs). This review highlights the application of aPDT against fish pathogens in the industry and the types of PSs utilized. It also explores the potential application of this technique for treating and controlling fish infections, along with the advantages and limitations of its use in aquaculture production systems. Finally, a conclusion and future perspectives are provided.

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Photodynamic effect of TPP encapsulated in polystyrene nanoparticles toward multi-resistant pathogenic bacterial strains: AFM evaluation

Cited by 9 publications

References 46 publications

Antimicrobial Photodynamic Therapy: Latest Developments with a Focus on Combinatory Strategies

Antimicrobial Photodynamic Therapy: Latest Developments with a Focus on Combinatory Strategies

Biological Evaluation of Photodynamic Effect Mediated by Nanoparticles with Embedded Porphyrin Photosensitizer

Applications of Antimicrobial Photodynamic Therapy in Aquaculture: Effect on Fish Pathogenic Bacteria

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