Photodynamic antimicrobial effect of safranine O on an ex vivo periodontal biofilm

Voos, Amelia C; Kranz, Stefan; Tonndorf-Martini, Silke; Voelpel, Andrea; Sigusch, Holger H.; Staudte, Henrike; Albrecht, Volker; Sigusch, Bernd W.

doi:10.1002/lsm.22217

Cited by 45 publications

(43 citation statements)

References 55 publications

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“…Recently, PDT has been used as an alternative therapy for treatment of several pathologies, such as skin cancer [41], Cutaneous leishmaniasis [42], candidiasis [43], tooth decay [44] and periodontitis [45]. …”

Section: Application Of Pdt In Dentistrymentioning

confidence: 99%

The application of antimicrobial photodynamic therapy (aPDT) in dentistry: a critical review

et al. 2016

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In recent years there have been an increasing number of in vitro and in vivo studies that show positive results regarding antimicrobial photodynamic therapy (aPDT) used in dentistry. These include applications in periodontics, endodontics, and mucosal infections caused by bacteria present as biofilms. Antimicrobial photodynamic therapy is a therapy based on the combination of a non-toxic photosensitizer (PS) and appropriate wavelength visible light, which in the presence of oxygen is activated to produce reactive oxygen species (ROS). ROS induce a series of photochemical and biological events that cause irreversible damage leading to the death of microorganisms. Many light-absorbing dyes have been mentioned as potential PS for aPDT and different wavelengths have been tested. However, there is no consensus on a standard protocol yet. Thus, the goal of this review was to summarize the results of research on aPDT in dentistry using the PubMed database focusing on recent studies of the effectiveness aPDT in decreasing microorganisms and microbial biofilms, and also to describe aPDT effects, mechanisms of action and applications.

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Section: Application Of Pdt In Dentistrymentioning

confidence: 99%

The application of antimicrobial photodynamic therapy (aPDT) in dentistry: a critical review

et al. 2016

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“…Moreover, recently Voos et al (2014) examined Safranine O (3,7-diamino-2,8-dimethyl-5-phenyl-phenazinium chloride), a structure analog to classical phenothiazinium dyes, where a nitrogen atom replaces the sulfur atom, which centers the delocalized electron system, for aPDT against biofilms. In this study, ex vivo biofilms were cultured from subgingival plaque samples for 24 or 72 h. After the respective incubation period the biofilms were incubated with Safranine O (10 μ M) for 15 min followed by irradiation with a diode laser (0.5 W; 532 nm) for 40 or 100 s, applying light doses of 20 or 50 J/cm 2 , respectively.…”

Section: Apdt Against Biofilms In Vitromentioning

confidence: 99%

“…In 24 h old biofilms there was a reduction of 2 log 10 steps with a light dose of 20 J/cm 2 and 3 log 10 steps with 50 J/cm 2 , whereas treatment with CHX (0.2%; 3 min) generated a reduction by 1 log 10 step only. In contrast, in 72 h old biofilms neither aPDT nor treatment with CHX led to any antibacterial effect (Voos et al, 2014). (Please find a summary of all studies described in this section in Table 1).…”

Section: Apdt Against Biofilms In Vitromentioning

confidence: 99%

Antimicrobial photodynamic therapy for inactivation of biofilms formed by oral key pathogens

Cieplik¹,

Tabenski²,

Buchalla³

et al. 2014

Front. Microbiol.

191

129

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With increasing numbers of antibiotic-resistant pathogens all over the world there is a pressing need for strategies that are capable of inactivating biofilm-state pathogens with less potential of developing resistances in pathogens. Antimicrobial strategies of that kind are especially needed in dentistry in order to avoid the usage of antibiotics for treatment of periodontal, endodontic or mucosal topical infections caused by bacterial or yeast biofilms. One possible option could be the antimicrobial photodynamic therapy (aPDT), whereby the lethal effect of aPDT is based on the principle that visible light activates a photosensitizer (PS), leading to the formation of reactive oxygen species, e.g., singlet oxygen, which induce phototoxicity immediately during illumination. Many compounds have been described as potential PS for aPDT against bacterial and yeast biofilms so far, but conflicting results have been reported. Therefore, the aim of the present review is to outline the actual state of the art regarding the potential of aPDT for inactivation of biofilms formed in vitro with a main focus on those formed by oral key pathogens and structured regarding the distinct types of PS.

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“…However, this might cause unwanted side effects and possible antibiotic resistance. In addition, it is not easy to have an effective dose for the topical agents used to remove the biofilm [3,4,5]. These difficulties demonstrate that there was a strong need to develop alternative techniques to mitigate these problems.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Photodynamic Inactivation against Periodontal Bacteria Mediated by a Chitosan Hydrogel in a 3D Gingival Model

Peng

Hsieh

Chen

et al. 2016

IJMS

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Chitosan hydrogels containing hydroxypropyl methylcellulose (HPMC) and toluidine blue O were prepared and assessed for their mucoadhesive property and antimicrobial efficacy of photodynamic inactivation (PDI). Increased HPMC content in the hydrogels resulted in increased mucoadhesiveness. Furthermore, we developed a simple In Vitro 3D gingival model resembling the oral periodontal pocket to culture the biofilms of Staphylococcus aureus (S. aureus), Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans), and Porphyromonas gingivalis (P. gingivalis). The PDI efficacy of chitosan hydrogel was examined against periodontal biofilms cultured in this 3D gingival model. We found that the PDI effectiveness was limited due to leaving some of the innermost bacteria alive at the non-illuminated site. Using this 3D gingival model, we further optimized PDI procedures with various adjustments of light energy and irradiation sites. The PDI efficacy of the chitosan hydrogel against periodontal biofilms can significantly improve via four sides of irradiation. In conclusion, this study not only showed the clinical applicability of this chitosan hydrogel but also the importance of the light irradiation pattern in performing PDI for periodontal disease.

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Photodynamic antimicrobial effect of safranine O on an ex vivo periodontal biofilm

Cited by 45 publications

References 55 publications

The application of antimicrobial photodynamic therapy (aPDT) in dentistry: a critical review

The application of antimicrobial photodynamic therapy (aPDT) in dentistry: a critical review

Antimicrobial photodynamic therapy for inactivation of biofilms formed by oral key pathogens

Assessment of Photodynamic Inactivation against Periodontal Bacteria Mediated by a Chitosan Hydrogel in a 3D Gingival Model

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