Photodynamic activity of anthraquinones isolated from Heterophyllaea pustulata Hook f. (Rubiaceae) on MCF-7c3 breast cancer cells

Comini, Laura R.; Fernandez, I.M.; Vittar, Natalia Belén Rumie; Montoya, Susana C. Núñez; Cabrera, José Luis; Rivarola, Viviana

doi:10.1016/j.phymed.2011.05.008

Cited by 47 publications

(32 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…On the contrary, necrosis causes inflammation by destroying the cell membrane, resulting in cell swelling and spillage of intracellular materials into the surrounding environment [13]. Apoptotic cell death is morphologically characterized by cell shrinkage, membrane blebbing, cell budding, and formation of apoptotic bodies without plasma membrane breakdown [14]. In this study, we observed photodynamic-mediated cell death by introverted microscopy.…”

Section: Bnce Induces Morphological Change Of Cancer Cellsmentioning

confidence: 96%

See 1 more Smart Citation

Photodynamic apoptosis and antioxidant activities of Brassica napus extracts in U937 and SK-HEP-1 cells

et al. 2017

View full text Add to dashboard Cite

Brassica napus is the most common feedstock for biodiesel production, and its cultivation area has been rapidly increased. Thus, B. napus residues left in the field after harvest are valuable resources. However, there have been few studies on biologically active substances from B. napus plant. The objective of this study is to evaluate cytotoxicity/photodynamic activity and antioxidant activity of B. napus plant extracts. B. napus plants were sequentially extracted with organic solvents (hexane, chloroform, ethanol, and water) and then screened for antioxidant activity and cytotoxicity against leukemia U937 and human liver cancer SK-HEP-1 cells. Among the solvent extracts, the cytotoxicity was the highest when cells treated with chloroform extract and irradiated. Degree of apoptosis substantially increased in both cell types in concentrationdependent manner, and non-irradiated cells showed similar results as the control cells. For the highest concentrations (100 lg/ml), toxicity effect in U937 and SK-HEP-1 cells was 94.62 ± 0.15% and 74.16 ± 1.54%, respectively. We observed the number of cells significantly decreased, and vesicles were floating in B. napus chloroform extract (BNCE) and light condition. BNCE induced DNA laddering pattern (between 300 and 1000 bp) and caspase-3/7 activation in both U937 and SK-HEP-1 cells. Total apoptotic U937 and SK-HEP-1 cells following BNCE 100 lg/ ml and light treatment were significantly increased (92.62 ± 2.07% and 59.71 ± 4.38%, respectively) compared with control. Our results showed that U937 cells were more sensitive than SK-HEP-1 cells. For the antioxidant activity, B. napus ethanol extract was the highest (IC 50 = 0.52 mg/ml).

show abstract

Section: Bnce Induces Morphological Change Of Cancer Cellsmentioning

confidence: 96%

“…However, U937 and SK-HEP-1 cells showed no internucleosomal (200 bp) DNA. Several research groups reported large DNA fragments during apoptosis and proposed that large DNA fragmentation occurs before internucleosomal DNA cleavage, and these large fragments serve as precursors for smaller DNA fragments [14].…”

Section: Bnce Induces Dna Fragmentationmentioning

confidence: 99%

Photodynamic apoptosis and antioxidant activities of Brassica napus extracts in U937 and SK-HEP-1 cells

et al. 2017

View full text Add to dashboard Cite

show abstract

“…137 Correlation between reduction potential of quinones and their cytotoxic action is well known. 138,139 Quinones with high reduction potential favour the formation of semiquinone radical leading to O2 -. .…”

Section: Enzymatic Reduction Of Quinones and Its Cytotoxicitymentioning

confidence: 99%

Quinones as photosensitizer for photodynamic therapy: ROS generation, mechanism and detection methods

Rajendran¹

2016

Photodiagnosis and Photodynamic Therapy

View full text Add to dashboard Cite

“…The first three AQs, stand out as the main components of leaves and stems and have photosensitizing properties, mediated by the generation of superoxide anion (O 2− ; Type I mechanism) and/or singlet molecular oxygen ( 1 O 2 ; Type II mechanism) [4][5][6]. AQs show important antibacterial and anticancer activity in vitro by means of the photosensitizing phenomenon [7,8]. In addition, we have previously established that extracts containing these compounds exhibit a significant antibacterial, antifungal and antiviral activity in vitro without the involvement of a photosensitizing action [4,9].…”

Section: Introduction Q4mentioning

confidence: 99%

Pressurized hot water extraction of anthraquinones from Heterophyllaea pustulata Hook f. (Rubiaceae)

Vázquez

Comini

Milanesio

et al. 2015

The Journal of Supercritical Fluids

Self Cite

View full text Add to dashboard Cite

Photodynamic activity of anthraquinones isolated from Heterophyllaea pustulata Hook f. (Rubiaceae) on MCF-7c3 breast cancer cells

Cited by 47 publications

References 13 publications

Photodynamic apoptosis and antioxidant activities of Brassica napus extracts in U937 and SK-HEP-1 cells

Photodynamic apoptosis and antioxidant activities of Brassica napus extracts in U937 and SK-HEP-1 cells

Quinones as photosensitizer for photodynamic therapy: ROS generation, mechanism and detection methods

Pressurized hot water extraction of anthraquinones from Heterophyllaea pustulata Hook f. (Rubiaceae)

Contact Info

Product

Resources

About