Photodynamic action of porphyrins on amino acids and proteins. I. Selective photooxidation of methionine in aqueous solution

Jori, Giulio; Galiazzo, G.; Scoffone, Ernesto

doi:10.1021/bi00835a026

Cited by 71 publications

(26 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Activation of lysosomal enzymes has also been proposed to induce chromosomal damage (Allison & Paton, 1965). On the molecular level, damage is known to be induced in amino acids/proteins (Jori et al, 1969) and lipids (Mead, 1976). Some authors claim that membrane damage is the determining step in cell inactivation (Lamola, 1976).…”

mentioning

confidence: 99%

Photodynamic action and chromosomal damage: A comparison of haematoporphyrin derivative (HpD) and light with X-irradiation

Evensen¹,

Moan²

1982

Br J Cancer

View full text Add to dashboard Cite

Summary.-Chromosomal aberrations (CA) are induced in human cells (NHIK 3025) in vitro when exposed to X-rays and to haematoporphyrin derivative (HpD) plus light. At the 0-1 survival level X-rays induce about 10 times more breaks per chromosome than the photodynamic treatment. There is some evidence for nonrandom distribution of the CA induced by HpD plus light; i.e. they seem to be localized at the centromeric and telomeric regions. Such non-random distribution of CA could be explained if centromeric and telomeric chromatin were associated with the inner nuclear membrane.

show abstract

mentioning

confidence: 99%

Photodynamic action and chromosomal damage: A comparison of haematoporphyrin derivative (HpD) and light with X-irradiation

Evensen¹,

Moan²

1982

Br J Cancer

View full text Add to dashboard Cite

show abstract

“…This product was identified as Met0 and Z-MetO-Asp, respectively, on the basis of the Rfvalues in several solvent systems [7]. The photoreaction was complete within 2 hr or irradiation.…”

Section: Resultsmentioning

confidence: 98%

Acetone‐sensitized anaerobic photo‐oxidation of methionine

Gennari

Jori

1970

FEBS Letters

View full text Add to dashboard Cite

“…Based on studies of photosensitized lysosomal damage, Slater and Riley suggested that the biochemical mode of action of a photosensitizer was the initiation of free radical reactions of the peroxy type with resultant damage to cellular membranes (38). Studies of the photochemistry of porphyrins include the observation of photoinduced porphyrin free radicals by Mauzerall and Feher (39), the detection of electron transfer from irradiated protoporphyrin IX (8), and the demonstration of the photodynamic action of porphyrins on amino acids and proteins (40).…”

Section: Discussionmentioning

confidence: 99%

“…Venous blood was obtained from five subjects with EPP, two of whom were related. Free erythrocyte protoporphyrin levels, measured by the method of Wranne (9), ranged from 700 to 1360 /ug/100 ml (normal < 40 Ag/100 ml). Hematocrits and reticulocyte counts of these patients were within normal limits.…”

Section: Methodsmentioning

confidence: 99%

Erythropoietic Protoporphyria: Lipid Peroxidation and Red Cell Membrane Damage Associated with Photohemolysis

Goldstein¹,

Harber²

1972

J. Clin. Invest.

166

View full text Add to dashboard Cite

A B S T R A C T The mechanism by which long wavelength ultraviolet light hemolyzes red cells obtained from patients with erythropoietic protoporphyria (EPP) was investigated. Previous studies had suggested that irradiation of these red cells with wavelengths of light capable of eliciting dermatological manifestations led to oxygen-dependent colloid osmotic hemolysis through the formation of peroxides. In the present report, lipid peroxidation during in vitro irradiation of EPP red cells with long ultraviolet light was demonstrated by: (a) the formation of 2-thiobarbituric acid reactants; (b) the presence of conjugated diene bonds in red cell lipid; and (c) the selective loss of unsaturated fatty acids proportional to the number of carbon-carbon double bonds in each. Irradiation of EPP red cells was also shown to result in the formation of hydrogen peroxide.Before photohemolysis there was a decline in cell membrane sulfhydryl groups and a loss in activity of the cell membrane enzyme acetylcholinesterase. These parameters provide further evidence suggesting that the cell membrane is a primary site of the photohemolytic effect of long ultraviolet light in EPP red cells.Further evaluation of the radiation-induced inactivation of EPP red cell acetylcholinesterase was performed by radiating mixtures containing bovine erythrocyte acetylcholinesterase and protoporphyrin IX. These studies revealed that the rate of decline in enzyme activity is accelerated by the addition of linoleic acid, an unsaturated fatty acid, but not by palmitic acid, a saturated fatty acid.

show abstract

Photodynamic action of porphyrins on amino acids and proteins. I. Selective photooxidation of methionine in aqueous solution

Cited by 71 publications

References 33 publications

Photodynamic action and chromosomal damage: A comparison of haematoporphyrin derivative (HpD) and light with X-irradiation

Photodynamic action and chromosomal damage: A comparison of haematoporphyrin derivative (HpD) and light with X-irradiation

Acetone‐sensitized anaerobic photo‐oxidation of methionine

Erythropoietic Protoporphyria: Lipid Peroxidation and Red Cell Membrane Damage Associated with Photohemolysis

Contact Info

Product

Resources

About