Photodynamic ability of silver nanoparticles in inducing cytotoxic effects in breast and lung cancer cell lines

Elhussein, Ahmed; Harith, M. A.; Abrahamse, Heidi; Hussein, Ahmed; Tynga, Ivan Mfouo

doi:10.2147/ijn.s63371

Cited by 21 publications

(9 citation statements)

References 46 publications

(45 reference statements)

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“…Afterward, the specimens were serially dehydrated with graded ethanol (50%, 70%, 90%, and 100%), infiltrated with propylene oxide, and then embedded in Araldite-Epon mixture. The samples were cut into ultrathin (70 nm) sections with a glass knife on an LKB ultramicrotome and then double-stained on 200-mesh copper grids with 2% uranyl acetate and lead citrate for examination [ 41 ].…”

Section: Methodsmentioning

confidence: 99%

Oxidative Stress and Apoptotic Responses Elicited by Nostoc-Synthesized Silver Nanoparticles against Different Cancer Cell Lines

Hamida

Albasher

Bin-Meferij

2020

Cancers

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Green nanoparticles represent a revolution in bionanotechnology, providing opportunities to fight life-threatening diseases, such as cancer, with less risk to the environment and to human health. Here, for the first time, we systematically investigated the anticancer activity and possible mechanism of novel silver nanoparticles (N-SNPs) synthesized by Nostoc Bahar M against the MCF-7 breast cancer cells, HCT-116 colorectal adenocarcinoma cells, and HepG2 liver cancer cells, using cell viability assays, morphological characterization with inverted light and transmission electron microscopy, antioxidants and enzymes (glutathione peroxidase (GPx), glutathione (GSH), adenosine triphosphatase (ATPase), and lactate dehydrogenase (LDH)), and western blotting (protein kinase B (Akt), phosphorylated-Akt (p-Akt), mammalian target of rapamycin (mTOR), B-cell lymphoma 2 (Bcl-2), tumor suppressor (p53), and caspase 3). N-SNPs decreased the viability of MCF-7, HCT-116, and HepG2 cells, with half-maximal inhibitory concentrations of 54, 56, and 80 µg/mL, respectively. They also significantly increased LDH leakage, enhanced oxidative stress via effects on antioxidative markers, and caused metabolic stress by significantly decreasing ATPase levels. N-SNPs caused extensive ultrastructural alterations in cell and nuclear structures, as well as in various organelles. Furthermore, N-SNPs triggered apoptosis via the activation of caspase 3 and p53, and suppressed the mTOR signaling pathway via downregulating apoptosis-evading proteins in MCF-7, HCT-116, and HepG2 cells. Ultrastructural analysis, together with biochemical and molecular analyses, revealed that N-SNPs enhanced apoptosis via the induction of oxidative stress and/or through direct interactions with cellular structures in all tested cells. The cytotoxicity of Nostoc-mediated SNPs represents a new strategy for cancer treatment via targeting various cell death pathways. However, the potential of N-SNPs to be usable and biocompatible anticancer drug will depend on their toxicity against normal cells.

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Section: Methodsmentioning

confidence: 99%

Oxidative Stress and Apoptotic Responses Elicited by Nostoc-Synthesized Silver Nanoparticles against Different Cancer Cell Lines

Hamida

Albasher

Bin-Meferij

2020

Cancers

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“…Searching for more effective protocols for drug administration leads to the modification of already existing procedures and combining pharmacological agents with natural, unconventional molecules. Metal-based AgNPs, known as pro-oxidative in different cancer cell lines [36] including breast MCF-7 and lung A549 cells [37] and squamous carcinoma SCC-25 cells [12], have shown novel applications in photodynamic therapy [37,38]. The alkaloid berberine was tested on squamous carcinoma cells as an antiproliferative and pro-apoptotic agent alone [22,[39][40][41] or in combination with AgNPs that improved its anticancer properties [12].…”

Section: Anticancer Activitymentioning

confidence: 99%

“…DNA damage changed gene expression, and cellular death could be manifested as programmed death (apoptosis) [44]. In photodynamic therapy (PDT), AgNPs caused tumor cell sensitization via intracellular ROS overproduction [19,37,38]. Ag ions are captured by free electrons, which affect mitochondrial membrane potential (Ψ) and leads to an increase in mitochondrial membrane permeability [45].…”

Section: Pro-oxidative Activitymentioning

confidence: 99%

“…Metal-based particles, because of their paramagnetic property and optical density (Figure 2), are widely used in bio-imaging as well as in electron microscopy, in magnetic resonance, in computed tomography for visualization, and in molecular diagnostics [19,21,50]. AgNPs, as cellular sensitizers with pro-oxidative and pro-apoptotic potential, also serve as therapeutic agents in photodynamic therapy against cancer cells [37,38]. In future applications some possible controversies must be resolved: dosage for different tissues, because of tissue-specific biocompatibility and side effects during therapy or microbial resistance against NPs.…”

Section: Applications Of Agnpsmentioning

confidence: 99%

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Biological Activity of Silver Nanoparticles and Their Applications in Anticancer Therapy

Skonieczna¹,

Hudy²

2018

Silver Nanoparticles - Fabrication, Characterization and Applications

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Nanotechnology delivers materials and nanoparticles (NPs) with high biological potential, useful in bioengineering, nanomedicine, and human health protection. Silver nanoparticles (NPs), because of their wide spectrum of activities and physical and chemical properties, are nowadays extensively researched. However, careful studies on living organism should be performed, with strong attention to biocompatibility. Multiple cellular effects, displayed after AgNP treatments, show interesting potential of metal-based NPs, not only in bio-nanotechnology but also in molecular medicine and anticancer therapy. AgNPs are promising anticancer agents, influencing the cell cycle, inhibiting cancer proliferation, and inducing oxidative stress and propagation of programmed cellular death (apoptosis). Additionally, they protect against bacterial, fungal, and viral infections. During chemo-and radio-therapies, such antimicrobial protection will be desirable because of the decreased immunological resistance of cancer patients. In conclusion, AgNPs often present in the human environment should be studied for novel findings and better characteristic. This article discusses advantages of AgNP's "eco-friendly" production, followed by green synthesis, with particular consideration of antimicrobial and anticancer properties. Cellular processes, induced after AgNP treatments, are focused on antiproliferative, pro-oxidative, and pro-apoptotic activities of NPs.

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“…Metal-based silver nanoparticles (Ag-NPs) have sparked interest due to their unique physicochemical properties such as chemical stability and electrical conductivity (Sharma et al, 2009) and also biological properties, including anti-bacterial, antifungal, anti-inflammatory, anti-viral, anti-angiogenesis, anticancer, and anti-platelet properties (Wong and Liu, 2010;Krishnaraj et al, 2012;Monteiro et al, 2012). Ag-NPs exhibited intrinsic cytotoxic activity in tumor cells (Singh and Ramarao, 2012;El-Sonbaty, 2013) including the release of silver ions and induced oxidative stress in fibroblast and glioblastoma cells, HeLa cells, THP-1 monocytes, breast MCF-7, lung A549, and squamous carcinoma SCC-25 cells (Sonoda et al, 1998;Asharani et al, 2009;Foldbjerg et al, 2009;Abrahamse et al, 2014;Dziedzic et al, 2016;Dayem et al, 2017;El-Hussein and Hamblin, 2017). As a result, cellular pathways become dysregulated, resulting in increased cellular damage and apoptosis (Gomes et al, 2021) thus providing an insight toward the use of nanomedicines.…”

Section: Introductionmentioning

confidence: 99%

Role of Biosynthesized Ag-NPs Using Aspergillus niger (MK503444.1) in Antimicrobial, Anti-Cancer and Anti-Angiogenic Activities

et al. 2022

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Green synthesis of nanoparticles is regarded as a safe and non-toxic process over conventional synthesis. Owing to the medicinal value of biologically derived biomolecules and utilizing them in synergy with nanoscience to offer more accurate therapeutic options to various diseases is an emerging field. One such study we present here with highlights of the synthesis and efficacy of biogenic silver nanoparticles produced from the extract of Aspergillus niger SAP2211 (accession number: MK503444.1) as an antimicrobial, anti-cancerous and anti-angiogenic agent. The synthesized Ag-NPs were characterized following UV–vis, FTIR, XRD, SEM and TEM, and were found to possess bactericidal activity against the selected pathogenic microbes, such as Staphylococcus aureus, Escherichia coli, and Salmonella typhi. Further, we evaluated cytotoxicity effect of this biogenic Ag-NPs using MMT assay on normal cardio myoblast (H9C2) and cancerous human cervical carcinoma (HeLa) cells. Doxorubicin used as positive control. This Ag-NPs have shown trivial cytotoxicity at the IC50 concentration on normal cells (IC50 = 47.17 µg/ml) over the cancer cells (IC50 = 8.609 µg/ml) with nearly 7 fold difference, indicating it as a selective anti-cancerous agent in contrast to standard drug doxorubicin (IC50 = 6.338 µg/ml). Further in-vitro assessment of wound healing capability by scratch wound healing assay, invasion by transwell matrigel invasion assay, and apoptosis via DAPI and annexin V-FITC assays were studied in HeLa cells. Synthesized biogenic Ag-NPs have shown to be anti-angiogenic in nature, which was established by in-vivo chick chorioallantois membrane assay. Overall, in vitro studies revealed that biogenic Ag-NPs positively inhibited migration, invasion, and induced apoptosis, and in-vivo CAM assay revealed that intercapillary network was reduced and the angiogenesis was inhibited.

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Photodynamic ability of silver nanoparticles in inducing cytotoxic effects in breast and lung cancer cell lines

Cited by 21 publications

References 46 publications

Oxidative Stress and Apoptotic Responses Elicited by Nostoc-Synthesized Silver Nanoparticles against Different Cancer Cell Lines

Oxidative Stress and Apoptotic Responses Elicited by Nostoc-Synthesized Silver Nanoparticles against Different Cancer Cell Lines

Biological Activity of Silver Nanoparticles and Their Applications in Anticancer Therapy

Role of Biosynthesized Ag-NPs Using Aspergillus niger (MK503444.1) in Antimicrobial, Anti-Cancer and Anti-Angiogenic Activities

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