Photodissociation of trapped ions

Hart, W.J. van der

doi:10.1002/mas.1280080403

Cited by 48 publications

(20 citation statements)

References 109 publications

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“…3'42 Two peaks in the CF 3 Mn(CO) 5 neutral spectrum were attributed to C-F stretching modes of A 1 and E symmetry respectively, and shifts to lower frequency of eacn peak were seen in both CF 3 Mn(CC) 3 (NO)" and CF 3 Mn(CO) 4 ". Explanation for these shifts involved increase of the elec"tron density in the carbon adonor orbital of the CF 3 group in the anionic species.…”

Section: Discussionmentioning

confidence: 99%

“…The disappearance of parent ion or the appearance of the fragment ions as a function of irradiation wavelength can then lead to a photodissociation spectrum of the parent ion under favorable conditions. 5 This approach has exhibited reliable results for interpretation of energy levels, dissociation thresholds, and structures of gaseous ions, when they ale subjected to UV-visible irradiation, quite often in Fourier transform ion cyclotron resonance (FTICR) mass spectrometers. 6 -9 The technique of infrared multiple photon dissociation (IRMPD) of ions has been used in isolated instances by several researchers " to obtain spectra of gaseous ions in order to assist in determining ion structure.…”

Section: Tlrmentioning

confidence: 99%

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Infrared multiple photon dissociation spectra of gaseous ions

Peiris¹,

Cheeseman²,

Ramanathan³

et al. 1993

J. Phys. Chem.

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Section: Discussionmentioning

confidence: 99%

Section: Tlrmentioning

confidence: 99%

Infrared multiple photon dissociation spectra of gaseous ions

Peiris¹,

Cheeseman²,

Ramanathan³

et al. 1993

J. Phys. Chem.

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“…However, photodissociation of larger proteins (ubiquitin ions, 8.6 kDa) resulted in formation of small, uninformative fragment ions (Beu et al, 1993). Ultraviolet photodissociation of small ions has been extensively reviewed (Berkowitz, 1979;Dunbar, 1984Dunbar, , 2000Vanderhart, 1989Vanderhart, , 1992 and will not be further discussed in this review.…”

Section: Multiphoton Dissociationmentioning

confidence: 99%

Activation of large lons in FT‐ICR mass spectrometry

Laskin

Futrell

2004

Mass Spectrometry Reviews

177

146

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The advent of soft ionization techniques, notably electrospray and laser desorption ionization methods, has enabled the extension of mass spectrometric methods to large molecules and molecular complexes. This both greatly extends the applications of mass spectrometry and makes the activation and dissociation of complex ions an integral part of these applications. This review emphasizes the most promising methods for activation and dissociation of complex ions and presents this discussion in the context of general knowledge of reaction kinetics and dynamics largely established for small ions. We then introduce the characteristic differences associated with the higher number of internal degrees of freedom and high density of states associated with molecular complexity. This is reflected primarily in the kinetics of unimolecular dissociation of complex ions, particularly their slow decay and the higher energy content required to induce decomposition-the kinetic shift (KS). The longer trapping time of Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) significantly reduces the KS, which presents several advantages over other methods for the investigation of dissociation of complex molecules. After discussing general principles of reaction dynamics related to collisional activation of ions, we describe conventional ways to achieve single-and multiple-collision activation in FT-ICR MS. Sustained off-resonance irradiation (SORI)-the simplest and most robust means of introducing the multiple collision activation process-is discussed in greatest detail. Details of implementation of this technique, required control of experimental parameters, limitations, and examples of very successful application of SORI-CID are described. The advantages of high mass resolving power and the ability to carry out several stages of mass selection and activation intrinsic to FT-ICR MS are demonstrated in several examples. Photodissociation of ions from small molecules can be effected using IR or UV/vis lasers and generally requires tuning lasers to specific wavelengths and/ or utilizing high flux, multiphoton excitation to match energy levels in the ion. Photodissociation of complex ions is much easier to accomplish from the basic physics perspective. The quasi-continuum of vibrational states at room temperature makes it very easy to pump relatively large amounts of energy into complex ions and infrared multiphoton dissociation (IRMPD) is a powerful technique for characterizing large ions, particularly biologically relevant molecules. Since both SORI-CID and IRMPD are slow activation methods they have many common characteristics. They are also distinctly different because SORI-CID is intrinsically selective (only ions that have a cyclotron frequency close to the frequency of the excitation field are excited), whereas IRMPD is not (all ions that reside on the optical path of the laser are excited). There are advantages and disadvantages to each technique and in many applications they complement each other. In contrast wi...

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“…This phenomenon is well known; laser photodissociation of organic chromophores stored in mass spectrometers has been studied extensively since the 1970s, primarily in FT-ICR mass spectrometers [40][41][42][43]. Even if a single photon is insufficient to cause fragmentation, photodissociation can still be effected by the gradual buildup of internal energy in a molecule via the absorption of multiple photons, followed rapidly by the intramolecular vibrational redistribution (IVR) of energy.…”

Section: Introductionmentioning

confidence: 99%

“…Multiple photon dissociation is a slow process with a rate that depends on the rates of activation and deactivation (both radiative and collisional). Kinetic models describing the appearance of fragment ions and the disappearance of the precursor ion population as a function of pressure, laser fluence, and photon energy have been explored [17,38,[40][41][42][43]. The ability to observe directly dispersed fluorescence emission of visible light combined with mass-resolved photodissociation measurements provides the opportunity to understand in more detail the competition between fluorescence emission and alternative modes of deactivation.…”

Section: Introductionmentioning

confidence: 99%

Gas-Phase Fluorescence Excitation and Emission Spectroscopy of Three Xanthene Dyes (Rhodamine 575, Rhodamine 590 and Rhodamine 6G) in a Quadrupole Ion Trap Mass Spectrometer

Forbes

Jockusch

2011

J. Am. Soc. Mass Spectrom.

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The gas-phase fluorescence excitation, emission and photodissociation characteristics of three xanthene dyes (rhodamine 575, rhodamine 590, and rhodamine 6G) have been investigated in a quadrupole ion trap mass spectrometer. Measured gas-phase excitation and dispersed emission spectra are compared with solution-phase spectra and computations. The excitation and emission maxima for all three protonated dyes lie at higher energy in the gas phase than in solution. The measured Stokes shifts are significantly smaller for the isolated gaseous ions than the solvated ions. Laser power-dependence measurements indicate that absorption of multiple photons is required for photodissociation. Redshifts and broadening of the dispersed fluorescence spectra at high excitation laser power provide evidence of gradual heating of the ion population, pointing to a mechanism of sequential multiple-photon activation through absorption/emission cycling. The relative brightness in the gas phase follows the order R575 (1.00)GR590(1.15)GR6G(1.29). Fluorescence emission from several mass-selected product ions has been measured.

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Photodissociation of trapped ions

Cited by 48 publications

References 109 publications

Infrared multiple photon dissociation spectra of gaseous ions

Infrared multiple photon dissociation spectra of gaseous ions

Activation of large lons in FT‐ICR mass spectrometry

Gas-Phase Fluorescence Excitation and Emission Spectroscopy of Three Xanthene Dyes (Rhodamine 575, Rhodamine 590 and Rhodamine 6G) in a Quadrupole Ion Trap Mass Spectrometer

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