“…Aromatic nitro compounds, however, are well known for their insolubility, particularly in aqueous solutions, and chloramphenicol is no exception -it has low aqueous solubility ($2.5 g/L at 25 C and neutral pH), with its lowest solubility occurring at pH 5.5-7.0 (i.e. Chloramphenicol itself is stable, with a shelf life of several years for the crystalline form, and it retains 100% of its activity for 24 hours in solution over a pH range of 1-9.5 (Bartz, 1948); however, over a longer time period, it degrades in solution due to exposure to light (Zhou et al, 2010), heat (Franje et al, 2010), and different pHs (Boer and Pijnenburg, 1983;Zhou et al, 2010). Amide NH groups are usually considered to be neutral, but that of chloramphenicol is significantly more acidic than most, due to the electronegative influence of the two chlorine atoms causing greater than usual electron flow from the amide N to the carbonyl group.…”