Photoconstructs of Nerve Guidance Prosthesis Using Photoreactive Gelatin as a Scaffold

Abstract

We devised a novel nerve prosthesis composed of an elastomeric gelatinous tube and multifilament gelatinous fibers, both of which were prepared from styrene-derivatized gelatin, which allows in situ formation of a bioactive substance-incorporated gel. An in vitro study showed that the axonal regeneration potential of a photocured gelatin layer impregnated with laminin, fibronectin, and NGF was almost comparable with that of coated Matrigel. A nerve conduit and fibers prepared from photoreactive gelatin was subjected to visiblelight irradiation with rotation in the presence of camphorquinone as a photoinitiator using a custom-designed apparatus. A sample of transparent gelatinous conduit with an inner diameter of 1.2 mm and a wall thickness of 0.6 mm and gelatin fibers ranging from 10 to 100 µm in diameter were produced. The photocured elastomeric gelatinous tube was flexible and had structural integrity that allowed mechanical handling without breaking. A novel nerve guidance prosthesis composed of tubes packed with fibers was assembled. This photofabrication technology may enable the design of a tailor-made shape and rapid morphogenesis and functional recovery of damaged nerve tissue.

Photofabricated Gelatin-Based Nerve Conduits: Nerve Tissue Regeneration Potentials

“…fabricate such devices. The major advantage of our technology is that it is possible to fabricate devices of the In this article, our interest was extended to examining differences in nerve tissue regeneration potentials bedesired sizes in a wet state (12) plain tube (model I), a tube packed with a rod containing 2. Nerve tissue regeneration was enhanced in the following order: model III > model II > model I.…”

“…The distal stumps of the discamphorquinone. Details of the preparation of styrenated gelatin (21) and photofabrication of fibers, rods sected nerve were inserted into the distal end of the photocured gelatin conduit and fixed with epineurial and conduits are described in our previous studies (12). Briefly, visible-light irradiation was conducted using an sutures.…”

“…Three types of nerve prostheconduits and microfilament fibers can be photoconstructed by photopolymerization of styrenated gelatin sis were evaluated. The rats were anesthetized by intraperitoneal injection of sodium pentobarbital (Nembutal) using custom-designed equipment, and that laminin, fibronectin, and NGF, which can all be coimmobilized in solution at a dose of 30 mg/kg body weight, and by intramuscular administration of ketamine hydrochloride a photocured gelatin matrix, can promote axon migration and growth in vitro (12). In this study, three nerve (Ketalar) also at a dose of 30 mg/kg body weight.…”

The response of normal human osteoblasts to anionic polysaccharide polyelectrolyte complexes

“…For more than a decade, engineering of new tissues by using selective cell transplantation on polymer scaffolds as an artificial ECM instead of tissue transplantation to other living bodies has been studied [3,4]. Recently, many studies on developing a scaffold for tissue regeneration have been done using ECM proteins such as collagen and gelatin [5][6][7], biodegradable synthetic polymers [8][9][10] and polysaccharides [11,12]. Because proteins derived from human tissues have many problems such as antigenicity or potential for infection, a biocompatible synthetic polymer or polysaccharide may be preferable for tissue regeneration.…”

Study on Small Gap Sleeve Bridging Peripheral Nerve Injury

“…We were seeking a kind of new material with better biocompatibility that can facilitate the nerve regeneration effects. With the development of biomaterial science, several kinds of biodegradable materials were found and used in biomedical fields [18,19]. Some scholars even demonstrated that adding Schwann cells [20][21][22][23] or bone marrow stromal cells [24] in the conduit cavity can improve the nerve fibers regeneration effect.…”