The response of normal human osteoblasts to anionic polysaccharide polyelectrolyte complexes

Nagahata, Misao; Nakaoka, Ryusuke; Teramoto, Akira; Abe, Kôji; Tsuchiya, Toshie

doi:10.1016/j.biomaterials.2005.01.041

Cited by 18 publications

(9 citation statements)

References 30 publications

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“…HS-containing scaffolds stimulated MSC proliferation [104]. A porous collagen/C6S scaffold was used to support osteogenic differentiation of rat bone marrow cells [105,106]. Immobilized monosulfated HA facilitated the proliferation and TNAP activity of human osteoblasts in the same manner as a coating with collagen [97].…”

Section: Gags and Osteoblastsmentioning

confidence: 99%

Regenerative potential of glycosaminoglycans for skin and bone

et al. 2011

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To meet the growing need for tissue replacement materials for our aging population, the development of new adaptive biomaterials is essential. The tissues with the highest demand for implant materials are skin and bone. These tissues share various similarities, including signaling pathways and extracellular matrix composition. Glycosaminoglycans such as hyaluronan and chondroitin sulfate are the major organic extracellular matrix components. They modulate the attraction of skin and bone precursor cells and their subsequent differentiation and gene expression and regulate the action of proteins essential to bone and skin regeneration. The precise action of glycosaminoglycans varies according to their structural composition mainly in respect to the degree of sulfation and polymer length. Changes in the glycosaminoglycan composition are frequently seen in physiological and pathological remodeling processes, such as bone formation or scaring. Here, we review the current state of knowledge of how the most common glycosaminoglycan, chondroitin sulfate and hyaluronan, interact with bone and skin cells, and summarize their potential in tissue engineering for skeletal and skin diseases.

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Section: Gags and Osteoblastsmentioning

confidence: 99%

Regenerative potential of glycosaminoglycans for skin and bone

et al. 2011

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“…This approach has produced an extensive literature about the usefulness of bone substitutes made of type I collagen, 205-219 hyaluronan, 207,220-227 chondroitin sulfate (never used alone), [228][229][230][231][232][233][234][235][236][237][238][239][240][241][242][243] and their hybrids with ceramics [191][192][193][194]203,204,216,234 and other natural 228,[230][231][232][233][235][236][237][238] polymers. Among these, great attention was given to chitosan, [239][240][241][242] alginate, 207,243,244 gelatin, 216,245-248 silk, [249][250][251][252][253][254] and their hybrids. [255][256][257][258...…”

Section: Natural Polymersmentioning

confidence: 99%

From natural bone grafts to tissue engineering therapeutics: Brainstorming on pharmaceutical formulative requirements and challenges

Baroli

2009

Journal of Pharmaceutical Sciences

171

130

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“…In order to find an effective nanoparticle vehicle for delivery of growth factors, cytotoxic drugsor adhesion proteins to vascular endothelial cells, several factors such as composition, size, and charge of nanoparticles with regard to their endothelial binding, uptake, and possible cytotoxicity have to be analyzed [ 9 , 10 ]. Various nanoparticle systems composed of charged polysaccharides, polypeptides or synthetic polyelectrolytes have been used as drug delivery systems [ 11 , 12 , 13 ] and may also support cellular ingrowth into a scaffold material [ 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Interaction of Poly(l-lysine)/Polysaccharide Complex Nanoparticles with Human Vascular Endothelial Cells

et al. 2018

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Short TitlePolyelectrolyte nanoparticles and vascular endothelial cells.AbstractAngiogenesis plays an important role in both soft and hard tissue regeneration, which can be modulated by therapeutic drugs. If nanoparticles (NP) are used as vectors for drug delivery, they have to encounter endothelial cells (EC) lining the vascular lumen, if applied intravenously. Herein the interaction of unloaded polyelectrolyte complex nanoparticles (PECNP) composed of cationic poly(l-lysine) (PLL) and various anionic polysaccharides with human vascular endothelial cells (HUVEC) was analyzed. In particular PECNP were tested for their cell adhesive properties, their cellular uptake and intracellular localization considering composition and net charge. PECNP may form a platform for both cell coating and drug delivery. PECNP, composed of PLL in combination with the polysaccharides dextran sulfate (DS), cellulose sulfate (CS) or heparin (HEP), either unlabeled or labeled with fluorescein isothiocyanate (FITC) and either with positive or negative net charge were prepared. PECNP were applied to human umbilical cord vein endothelial cells (HUVEC) in both, the volume phase and immobilized phase at model substrates like tissue culture dishes. The attachment of PECNP to the cell surface, their intracellular uptake, and effects on cell proliferation and growth behavior were determined. Immobilized PECNP reduced attachment of HUVEC, most prominently the systems PLL/HEP and PLL/DS. A small percentage of immobilized PECNP was taken up by cells during adhesion. PECNP in the volume phase showed no effect of the net charge sign and only minor effects of the composition on the binding and uptake of PECNP at HUVEC. PECNP were stored in endosomal vesicles in a cumulative manner without apparent further processing. During mitosis, internalized PECNP were almost equally distributed among the dividing cells. Both, in the volume phase and immobilized at the surface, PECNP composed of PLL/HEP and PLL/DS clearly reduced cell proliferation of HUVEC, however without an apparent cytotoxic effect, while PLL/CS composition showed minor impairment. PECNP have an anti-adhesive effect on HUVEC and are taken up by endothelial cells which may negatively influence the proliferation rate of HUVEC. The negative effects were less obvious with the composition PLL/CS. Since uptake and binding for PLL/HEP was more efficient than for PLL/DS, PECNP of PLL/HEP may be used to deliver growth factors to endothelial cells during vascularization of bone reconstitution material, whereas those of PLL/CS may have an advantage for substituting biomimetic bone scaffold material.

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The response of normal human osteoblasts to anionic polysaccharide polyelectrolyte complexes

Cited by 18 publications

References 30 publications

Regenerative potential of glycosaminoglycans for skin and bone

Regenerative potential of glycosaminoglycans for skin and bone

From natural bone grafts to tissue engineering therapeutics: Brainstorming on pharmaceutical formulative requirements and challenges

Interaction of Poly(l-lysine)/Polysaccharide Complex Nanoparticles with Human Vascular Endothelial Cells

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