Photochemically induced focal cochlear lesions in the guinea pig: I. DAB staining and SEM study

Jing, Gao; Hoshino, Tomoyuki; Iwasaki, Satoshi; Wu, Rong

doi:10.1002/(sici)1097-0029(19980515)41:4<323::aid-jemt5>3.0.co;2-r

Cited by 7 publications

(2 citation statements)

References 27 publications

(29 reference statements)

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“…We have already established a different model for inner ear microcirculatory disorders [7,9,14,23,24]. Vertical illumination in that model shed both the lateral wall and the organ of Corti, so that the inner and outer hair cells might have been exposed to the direct influence of the photochemical reaction.…”

Section: Introductionmentioning

confidence: 99%

A new model for investigating hair cell degeneration in the guinea pig following damage of the stria vascularis using a photochemical reaction

Ocho

Iwasaki

Umemura

et al. 2000

European Archives of Oto-Rhino-Laryngology

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We have established a new model for investigating the relationship between cochlear lateral wall damage and sensory cell degeneration in guinea pigs by using a photochemical reaction between the systemic injection of Rose Bengal (RB) and controlled green light irradiation to the cochlea. The photochemical reaction produced a reactive oxygen species, which then damaged the endothelium. This triggered platelet adhesion and aggregation at the site of endothelial injury to produce thrombi and affect microcirculation in the lateral wall at the site of irradiation. Changes were studied under a scanning electron microscope (SEM), and compound action potentials (CAP) were measured. SEM observations after tangential illumination of the cochlear wall revealed degeneration of the stria vascularis (SV). Specific morphological findings at 24 h included delayed degeneration of the outer hair cells concurrent with a significant increase in the CAP. Based on these findings, we suggest that degeneration of the SV was a direct result of the photochemical reaction, but CAP changes and sensory hair cell damage were secondarily caused by SV degeneration.

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Section: Introductionmentioning

confidence: 99%

A new model for investigating hair cell degeneration in the guinea pig following damage of the stria vascularis using a photochemical reaction

Ocho

Iwasaki

Umemura

et al. 2000

European Archives of Oto-Rhino-Laryngology

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“…DAB staining was performed according to the method reported by Gao et al [28]. In brief, animals were decapitated and the cerebrum of the rats was fixed for 48 h in the solution of 4% paraformaldehyde containing 30% sucrose at 4 C. 40 mm cryostat sections were obtained for DAB staining.…”

Section: Dab Stainingmentioning

confidence: 99%

Cerebrum Repair with PHPMA Hydrogel Immobilized with Neurite-Promoting Peptides in Traumatic Brain Injury of Adult Rat Model

Cui¹,

Tian²,

Fan

et al. 2003

Journal of Bioactive and Compatible Polymers

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A poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) hydrogel immobilized with the neurite-promoting peptide sequence of xIKVAVx was synthesized and its structure was characterized. The PHPMA-IKVAV hydrogel displayed an interconnected porous structure. The ability of the hydrogel to support axonal outgrowth in the injured adult rats cerebrum cavity and to promote tissue regeneration was evaluated. After implantation for 4, 6, 12 and 18 weeks, the brain sections were processed for histological staining. The observations of the sections showed that the polymer hydrogel provided a structural, three-dimensional continuity across the defect and favoured reorganization of local wound-repair cells, angiogenesis and axonal growth into

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Role of nitric oxide in focal microcirculation disorder of guinea pig cochlea

et al. 2001

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Photochemically induced focal cochlear lesions in the guinea pig: I. DAB staining and SEM study

Cited by 7 publications

References 27 publications

A new model for investigating hair cell degeneration in the guinea pig following damage of the stria vascularis using a photochemical reaction

A new model for investigating hair cell degeneration in the guinea pig following damage of the stria vascularis using a photochemical reaction

Cerebrum Repair with PHPMA Hydrogel Immobilized with Neurite-Promoting Peptides in Traumatic Brain Injury of Adult Rat Model

Role of nitric oxide in focal microcirculation disorder of guinea pig cochlea

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