Photochemical Reaction of 7,12-Dimethylbenz[a]anthracene (DMBA) and Formation of DNA Covalent Adducts

Yu, Hongtao; Yan, Jun; Jiao, Yujuan; Fu, Peter P.

doi:10.3390/ijerph2005010114

Cited by 19 publications

(14 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Comparison of the DNA adduct profiles indicates that the DNA adducts formed from photo-irradiation are different from the DNA adducts formed due to the reaction of DMBA metabolites with DNA. These results suggest that photoirradiation of DMBA can lead to genotoxicity through activation pathways different from those by microsomal metabolism of DMBA [49].…”

Section: Formation Of Dna Adductsmentioning

confidence: 80%

“…It has been found that upon light irradiation, anthracene, benz[a]anthracene (BA), pyrene, 5-methylbenz[a]anthracene, 7,12-dimethyl-BA (DMBA), and benzo[a]pyrene react with oxygen to form endoperoxides as intermediates and the corresponding quinones as the end products [17,[43][44][45][46][47][48][49][50]. These results indicate that quinones are the stable end photoproducts of PAH light irradiation.…”

Section: Reactive Pah Intermediatesmentioning

confidence: 87%

“…Photoirradiation of DMBA in the presence of calf thymus DNA followed by 32 P-postlabeling/TLC indicated that multiple DNA adducts were formed [49]. Comparison of the DNA adduct profiles indicates that the DNA adducts formed from photo-irradiation are different from the DNA adducts formed due to the reaction of DMBA metabolites with DNA.…”

Section: Formation Of Dna Adductsmentioning

confidence: 99%

“…The photomutagenicity of sixteen PAHs, all on the US EPA Priority Pollutant List, was studied [56] [49].…”

Section: Photomutagenicitymentioning

confidence: 99%

See 3 more Smart Citations

Photoirradiation of Polycyclic Aromatic Hydrocarbons with UVA Light – A Pathway Leading to the Generation of Reactive Oxygen Species, Lipid Peroxidation, and DNA Damage

Xia

Yan

et al. 2006

IJERPH

Self Cite

View full text Add to dashboard Cite

Polycyclic aromatic hydrocarbons (PAHs) are a class of genotoxic environmental contaminants. We have long been interested in determining the mechanisms by which PAHs induce genotoxicity. Although the metabolic activation of PAHs leading to biological activities has been well studied, the photo-induced activation pathway has seldom reported. In this paper, we review the study of photoirradiation of PAHs with UVA irradiation results in (i) cytotoxicity and DNA damage (ii) DNA single strand cleavage; (iii) formation of 8-hydroxy-2'-deoxyguanosine adduct (8-OHdG), and (iv) formation of lipid peroxidation. Evidence has been shown that these photobiological activities are mediated by reactive oxygen species (ROS).

show abstract

Section: Formation Of Dna Adductsmentioning

confidence: 80%

Section: Reactive Pah Intermediatesmentioning

confidence: 87%

Section: Formation Of Dna Adductsmentioning

confidence: 99%

“…The photomutagenicity of sixteen PAHs, all on the US EPA Priority Pollutant List, was studied [56] [49].…”

Section: Photomutagenicitymentioning

confidence: 99%

See 2 more Smart Citations

Photoirradiation of Polycyclic Aromatic Hydrocarbons with UVA Light – A Pathway Leading to the Generation of Reactive Oxygen Species, Lipid Peroxidation, and DNA Damage

Xia

Yan

et al. 2006

IJERPH

Self Cite

View full text Add to dashboard Cite

show abstract

“…6,7 7,12-dimetilbenz[a]antrasen (DMBA) merupakan golongan Polycyclic Aromatic Hydrocarbon (PAH) yang diketahui bersifat sebagai karsinogen pada manusia. 8 Oksidasi metabolik DMBA akan menghasilkan metabolik epoksida yang sangat reaktif sehingga mampu memicu karsinogenesis. 9 Metabolit reaktif DMBA akan membentuk adduct pada DNA sehingga terjadi mutasi DNA yang menyebabkan perubahan sifat sel-sel normal menjadi sel kanker yang tak terkontrol.…”

Section: Pendahuluanunclassified

Studi in vivo ekstrak etanolik ciplukan (Physalis angulata) dalam meningkatkan apoptosis sel kanker lidah

Safitri

Nawangsih

Noviyanti

et al. 2016

Majalah Kedokteran Gigi Indonesia

View full text Add to dashboard Cite

ABSTRAKKanker lidah merupakan jenis kanker di dalam rongga mulut yang sering terjadi dan memiliki prevalensi yang tinggi. Ciplukan (Physalis angulata L.) memiliki aktivitas sitotoksik pada sel kanker, yaitu mampu menghambat proliferasi sel kanker dan menginduksi cell cycle arrest. Penelitian ini bertujuan mengkaji potensi ekstrak etanolik ciplukan (EEC) sebagai antikanker lidah dan mengetahui pengaruh EEC dalam meningkatkan apoptosis sel kanker lidah yang diinjeksi DMBA. Lima belas ekor tikus Sprague dawley betina usia 2-3 bulan dibagi menjadi tiga kelompok yaitu: (1) kelompok kontrol DMBA; (2) kelompok DMBA+EEC dosis 750 mg/kg BB; (3) kelompok DMBA+EEC dosis 1500 mg/kg BB. Kanker lidah diinduksi dengan injeksi 0,1 ml/100 gram BB tikus larutan DMBA 2% pada bagian lateral lidah hewan uji satu kali, lalu dibiarkan selama 5 minggu. Pada awal minggu ke-6 EEC 750 dan 1500 mg/kg BB diberikan secara sondasi kepada hewan uji selama 7 hari. Pada awal minggu ketujuh tikus dikorbankan. Selanjutnya, dilakukan pengamatan untuk memeriksa apoptosis sel dengan pewarnaan TdT-mediated X-dUTP Nick End Labeling (TUNEL). Hasil penelitian menunjukkan indeks apoptosis kelompok kontrol adalah 3,94%, kelompok perlakuan EEC 750 mg/kg BB adalah 15,34%, dan kelompok perlakuan EEC 1500 mg/kg BB sebesar 25,5%. Hasil uji One Way ANOVA (p<0,05) menunjukkan bahwa EEC mampu meningkatkan apoptosis sel kanker lidah tikus yang diinduksi DMBA. Kesimpulan penelitian ini adalah EEC dapat menghambat pertumbuhan sel kanker lidah dengan cara meningkatkan apoptosis sel kanker lidah tikus. 4 Faktor yang mempengaruhi terjadinya kanker lidah yaitu faktor lokal meliputi kebersihan rongga mulut yang buruk, iritasi kronis dari restorasi, dan karies gigi, sedangkan faktor luar antara lain kebiasaan merokok, minum alkohol,

show abstract

Sequence‐Specific DNA Photosplitting of Crosslinked DNAs Containing the 3‐Cyanovinylcarbazole Nucleoside by Using DNA Strand Displacement

2016

View full text Add to dashboard Cite

An oligodeoxynucleotide (ODN) containing the ultrafast reversible 3-cyanovinylcarbazole ((CNV) K) photo-crosslinker was photo-crosslinked to a complementary strand upon exposure to 366 nm irradiation and photosplit by use of 312 nm irradiation. In this paper we report that the photoreaction of (CNV) K on irradiation at 366 nm involves a photostationary state and that its reaction can be controlled by temperature. Guided by this new insight, we proposed and have now demonstrated previously unknown photosplitting of (CNV) K aided by DNA strand displacement as an alternative to heating. The photo-crosslinked double-stranded DNA (dsDNA) underwent >80 % photosplitting aided by DNA strand displacement on irradiation at 366 nm without heating. In this photosplitting based on DNA strand displacement, the relative thermal stability of the invader strand with respect to the template strands plays an important role, and an invader strand/template strand system that is more stable than the passenger strand/template strand system induces photosplitting without heating. This new strand-displacement-aided photosplitting occurred in a sequence-specific manner through irradiation at 366 nm in the presence of an invader strand.

show abstract

Photochemical Reaction of 7,12-Dimethylbenz[a]anthracene (DMBA) and Formation of DNA Covalent Adducts

Cited by 19 publications

References 26 publications

Photoirradiation of Polycyclic Aromatic Hydrocarbons with UVA Light – A Pathway Leading to the Generation of Reactive Oxygen Species, Lipid Peroxidation, and DNA Damage

Photoirradiation of Polycyclic Aromatic Hydrocarbons with UVA Light – A Pathway Leading to the Generation of Reactive Oxygen Species, Lipid Peroxidation, and DNA Damage

Studi in vivo ekstrak etanolik ciplukan (Physalis angulata) dalam meningkatkan apoptosis sel kanker lidah

Sequence‐Specific DNA Photosplitting of Crosslinked DNAs Containing the 3‐Cyanovinylcarbazole Nucleoside by Using DNA Strand Displacement

Contact Info

Product

Resources

About