Sequence‐Specific DNA Photosplitting of Crosslinked DNAs Containing the 3‐Cyanovinylcarbazole Nucleoside by Using DNA Strand Displacement

Abstract

An oligodeoxynucleotide (ODN) containing the ultrafast reversible 3-cyanovinylcarbazole ((CNV) K) photo-crosslinker was photo-crosslinked to a complementary strand upon exposure to 366 nm irradiation and photosplit by use of 312 nm irradiation. In this paper we report that the photoreaction of (CNV) K on irradiation at 366 nm involves a photostationary state and that its reaction can be controlled by temperature. Guided by this new insight, we proposed and have now demonstrated previously unknown photosplitting of (CNV) K aided by DNA strand displacement as an alternative to heating. The photo-crosslinked double-stranded DNA (dsDNA) underwent >80 % photosplitting aided by DNA strand displacement on irradiation at 366 nm without heating. In this photosplitting based on DNA strand displacement, the relative thermal stability of the invader strand with respect to the template strands plays an important role, and an invader strand/template strand system that is more stable than the passenger strand/template strand system induces photosplitting without heating. This new strand-displacement-aided photosplitting occurred in a sequence-specific manner through irradiation at 366 nm in the presence of an invader strand.

Molecular Cybernetics: Challenges toward Cellular Chemical Artificial Intelligence

“…In particular, the innovation of functional molecular materials is quite effective in designing recursive reaction mechanisms. For example, photo‐responsive molecules such as azobenzene [ 46 ] and 3‐cyanovinylcarbazole (cnvK) [ 47 ] switch their structural conformations when irradiated with UV light of specific wavelengths. The double helix of azobenzene‐modified DNA can be destabilized by UV irradiation through photoisomerization.…”