Photochemical inactivation of selected viruses and bacteria in platelet concentrates using riboflavin and light

Abstract

The data indicate that the device successfully reduced the number of selected pathogens in PCs. Despite the fact that significant differences exist between treated and control in-vitro variables, it is speculated that the clinical effectiveness of both products will not be significantly different, based on comparison to historical data for products in routine clinical use today.

Study of CD69 antigen expression and integrity of leukocyte cellular membrane in stored platelet concentrates following irradiation and treatment with Mirasol® PRT System

“…Following illumination, the PCs were stored for 6 days. 17 The study procedure ran as follows; 35 mL of 500 µM riboflavin solution was added to the PCs in group M, 35 mL of saline solution (0.9% NaCl, Ravimed, Poland) was added to the PCs in groups R and C. The M group bags were immediately illuminated for about 6 min at 6.24 J/mL. At the same time, the R group bags were irradiated and put in storage for 6 days at 22°C with agitation (Helmer, Fresenius Kabi, USA) and the C group bags were put in storage for 6 days at 22°C with agitation.…”

Strategies to Preclude Hepatitis C Virus Entry

“…The two photoinactivation methods applied to plasma could nevertheless be adapted to the inactivation of HCV and other viruses in platelet concentrates [170][171][172].…”

“…These small molecules can penetrate membranes and intercalate with helical regions of HCV nucleic acids. Subsequent UV illumination irreversibly alters nucleic acids, making HCV particles unable to replicate [154,170].…”

Transfusion-Associated Bacterial Sepsis

“…The method is believed to be effective if the number of bacteria is diminished by 5 -6 log 10 in reference to the baseline values (71,72). In in vitro studies, the parameter defining the effectiveness of the method is the reduction index (R), which is a negative logarithm (Y) of the ratio between the number of bacteria in the baseline material to the number of bacteria after reduction plus/minus 1:…”