Photobiomodulatory effects of superpulsed 904 nm laser therapy on bioenergetics status in burn wound healing

Yadav, Anju; Gupta, Asheesh; Keshri, Gaurav K.; Verma, Saurabh; Sharma, Sanjeev; Singh, Shashi Bala

doi:10.1016/j.jphotobiol.2016.06.031

Cited by 29 publications

(12 citation statements)

References 25 publications

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“…In the present study, pulsed 810 nm PBMT was found to reduce the oxidative stress by increasing NADPH and GRP78 levels and decreasing NADP/NADPH ratio and HNE adduct levels, thereby maintaining the cellular redox homeostasis. These findings are in accordance with the previous studies, which exhibited that superpulsed 904 nm PBM maintained the cellular redox homeostasis during burn wound healing . Likewise, other studies reported that PBMT reduced oxidative stress during healing of diabetic wounds and muscle injury .…”

Section: Discussionsupporting

confidence: 93%

“…Cellular redox homeostasis plays its role to cope‐up with oxidative stress during repair process. NADPH has been known to maintain redox homeostasis by providing reducing equivalent to the cell and also regulates various biological processes including carbohydrate metabolism, mitochondrial, immunological functions, and calcium signaling . Similarly, GRP78, a member of heat shock protein 70 family, gets involved in maintaining the cellular redox status by stabilizing the protein‐folding process .…”

Section: Discussionmentioning

confidence: 99%

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Effects of Pulsed 810 nm Al‐Ga‐As Diode Laser on Wound Healing Under Immunosuppression: A Molecular Insight

et al. 2019

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Background and Objectives Dysregulated inflammation is one of the major contributing factors for the prevalence of non‐healing chronic wound in immunosuppressed subjects. Photobiomodulation (PBM) has emerged as a potential non‐thermal, light‐based therapeutic healing intervention for the treatment of impaired wounds. Study Design/Materials and Methods The present study delineates the underlying molecular mechanisms of PBM 810 nm laser‐induced full‐thickness cutaneous wound repair in immunosuppressed rats at continuous and pulsed wave‐mode with power‐density of 40 mW/cm 2, fluence 22.6 J/cm 2 for 10 minutes daily for 7 post‐wounding days. Molecular markers were assessed using biochemical, enzyme‐linked immunosorbent assay quantification, enzyme kinetics and immunoblots analyses pertaining to inflammation, oxidative stress, cell survival, calcium signaling, and proliferation cascades. Results Results distinctly revealed that pulsed 810 nm (10 Hz) PBM potentially influenced the cell survival and proliferation signaling pathway by significantly upregulated phospho‐protein kinase B(phospho‐Akt), phospho‐extracellular‐signal‐regulated kinase 1 (ERK1), transient receptor potential vanilloid‐3 (TRPV3), Ca2+, calmodulin, transforming growth factor‐β1 (TGF‐β1), TGF‐βR3, and Na +/K +‐ATPase pump levels. PBM treatment resulted in reduction of exaggerated inflammatory responses evident by significantly repressed levels of interleukin‐1β (IL‐1β), IL‐6, cyclooxygenase 2 (COX‐2), and substance‐P receptor (SPR), as well as inhibited apoptotic cell death by decreasing p53, cytochrome C, and caspase 3 levels (P < 0.05), which, in turn, effectively augment the wound repair in immunosuppressed rats. PBM treatment also lowered 4‐hydroxynoneal (HNE) adduct level and NADP/NADPH ratio and upregulated the GRP78 expression, which might culminate into reduced oxidative stress and maintained the redox homeostasis. Conclusions Taken together, these findings would be helpful in better understanding of the molecular aspects involved in pulsed 810 nm laser‐mediated dermal wound healing in immunosuppressed rats through regulation of cell survival and proliferation via Ca2+‐calmodulin, Akt, ERK, and redox signaling. Lasers Surg. Med. © 2019 Wiley Periodicals, Inc.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Effects of Pulsed 810 nm Al‐Ga‐As Diode Laser on Wound Healing Under Immunosuppression: A Molecular Insight

et al. 2019

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“…A previous in vitro study suggested that high‐frequency diode laser irradiation stimulates human gingival epithelial cell proliferation and migration, in association with activation of the MAPK/ERK signaling pathway . Recently, several in vivo studies demonstrated that ultrashort pulses of laser irradiation (904 nm, average power: 0.7 mW; peak power: 35 W, 100 Hz; pulse duration: 200 ns, 0.2 J/cm 2 ) accelerates healing of burn wounds . In addition, it has been suggested that high‐frequency diode laser irradiation (910 nm, average power: 0.2 W; peak power: 45 W, 30 kHz; pulse duration: 200 ns, 17.4, 43.8 J/cm 2 ) promotes epithelialization and bone formation in tooth extraction sockets, possibly through activation of both cell proliferation and differentiation .…”

Section: Discussionmentioning

confidence: 99%

Effect of high‐frequency near‐infrared diode laser irradiation on periodontal tissues during experimental tooth movement in rats

et al. 2018

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“…Antimicrobial phototherapy (PT) can utilize both visible and non-visible light (ultraviolet and near-infrared), to treat infectious diseases in a minimally invasive manner. Light irradiation has been indicated to cause improvement in the healing process of burn wounds [117–123]. PT consists of several therapeutic methods such as UV irradiation (200–280 nm), blue light therapy (BLT) (400–470 nm) and low-level laser therapy (LLLT) (390–1100 nm).…”

Section: Antimicrobial Light and Ultrasound-based Wound Therapymentioning

confidence: 99%

“…LLLT has been tested in studies to treat several disorders, and was shown to increase cellular proliferation, modulate inflammation, and accelerate re-epithelization and repair of the injured skin [125, 134]. In one study by Yadav et al [117] a super-pulsed 904 nm laser (200 ns pulse width, 100 Hz, and mean output power equal to 0.7 mW) was used to increase ATP levels in burn wounds. The results indicated improved aerobic metabolism (by increasing the activity of enzymes e.g.…”

Section: Antimicrobial Light and Ultrasound-based Wound Therapymentioning

confidence: 99%

Nanomedicine and advanced technologies for burns: Preventing infection and facilitating wound healing

Jahromi

Zangabad

Basri

et al. 2018

Advanced Drug Delivery Reviews

375

189

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According to the latest report from the World Health Organization, an estimated 265,000 deaths still occur every year as a direct result of burn injuries. A widespread range of these deaths induced by burn wound happens in low- and middle-income countries, where survivors face a lifetime of morbidity. Most of the deaths occur due to infections when a high percentage of the external regions of the body area is affected. Microbial nutrient availability, skin barrier disruption, and vascular supply destruction in burn injuries as well as systemic immunosuppression are important parameters that cause burns to be susceptible to infections. Topical antimicrobials and dressings are generally employed to inhibit burn infections followed by a burn wound therapy, because systemic antibiotics have problems in reaching the infected site, coupled with increasing microbial drug resistance. Nanotechnology has provided a range of molecular designed nanostructures (NS) that can be used in both therapeutic and diagnostic applications in burns. These NSs can be divided into organic and non-organic (such as polymeric nanoparticles (NPs) and silver NPs, respectively), and many have been designed to display multifunctional activity. The present review covers the physiology of skin, burn classification, burn wound pathogenesis, animal models of burn wound infection, and various topical therapeutic approaches designed to combat infection and stimulate healing. These include biological based approaches (e.g. immune-based antimicrobial molecules, therapeutic microorganisms, antimicrobial agents, etc.), antimicrobial photo- and ultrasound-therapy, as well as nanotechnology-based wound healing approaches as a revolutionizing area. Thus, we focus on organic and non-organic NSs designed to deliver growth factors to burned skin, and scaffolds, dressings, etc. for exogenous stem cells to aid skin regeneration. Eventually, recent breakthroughs and technologies with substantial potentials in tissue regeneration and skin wound therapy (that are as the basis of burn wound therapies) are briefly taken into consideration including 3D-printing, cell-imprinted substrates, nano-architectured surfaces, and novel gene-editing tools such as CRISPR-Cas.

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Photobiomodulatory effects of superpulsed 904 nm laser therapy on bioenergetics status in burn wound healing

Cited by 29 publications

References 25 publications

Effects of Pulsed 810 nm Al‐Ga‐As Diode Laser on Wound Healing Under Immunosuppression: A Molecular Insight

Effects of Pulsed 810 nm Al‐Ga‐As Diode Laser on Wound Healing Under Immunosuppression: A Molecular Insight

Effect of high‐frequency near‐infrared diode laser irradiation on periodontal tissues during experimental tooth movement in rats

Nanomedicine and advanced technologies for burns: Preventing infection and facilitating wound healing

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