Photobiomodulation therapy reverses spermatogenesis arrest in hyperthermia-induced azoospermia mouse model

Ziaeipour, Sanaz; Norouzian, Mohsen; Abbaszadeh, Hojjat-Allah; Aliaghaei, Abbas; Nazarian, Hamid; Karamian, Amin; Tabeie, Faraj; Naserzadeh, Parvaneh; Abdi, Shabnam; Abdollahifar, Mohammad‐Amin; Paktinat, Shahrokh

doi:10.1007/s10103-023-03780-8

Cited by 6 publications

(2 citation statements)

References 29 publications

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“…Simultaneously, PBMT treatment reduced ROS levels, mitochondrial membrane potential, and lipid peroxidation in the azoospermia model. 32 The findings demonstrated a significant increase in the levels of GSSG and ROS in the hyperthermic group as compared to the control group. However, no significant difference was observed between the control and treatment groups.…”

Section: Discussionmentioning

confidence: 86%

Therapeutic Effects of Exosome Therapy and Photobiomodulation Therapy on the Spermatogenesis Arrest in Male Mice After Scrotum Hyperthermia

Tabatabaee,

Darabi,

Soltani

et al. 2024

J Lasers Med Sci

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Introduction: In men, several factors cause infertility, among which we can mention damage to sperm due to high temperature. So far, various treatments have been proposed for it, but they have not been highly effective. The current study aimed to evaluate the effect of exosome therapy (EXO) and photobiomodulation therapy (PBMT) on spermatogenesis arrest in male mice after scrotum hyperthermia. Methods: In this experimental study, the animals were divided into four groups: control, scrotal hyperthermia, scrotal hyperthermia+EXO (100 μL/d) (mice were treated for 30 days), scrotal hyperthermia+PBMT (laser of 0.03 J/cm2 for 30 seconds/for 30 days). Hyperthermia was induced by exposure to the temperature of 43 °C for 20 minute every day for 5 times. After 6 weeks, the animals were sacrificed. Results: The treated groups showed a significant increase in sperm parameters, as compared to the hyperthermic groups. Moreover, these favorable effects were observed in relation to the volume of testicular tissue, the number of germ cells, Leydig cells and Sertoli cells, and the level of testosterone. Research on antioxidants showed a significant reduction in oxidized glutathione (GSSG) and reactive oxygen species (ROS) in the treatment groups in comparison to the hyperthermia group (P<0.001). Also, there has been a significant increase in the amount of hydrogen peroxide enzyme observed in the hyperthermia group as opposed to the treatment group (P<0.001). Conclusion: These findings show that EXO and PBMT can improve spermatogenesis caused by hyperthermia, reduce ROS and GSSG, and increase glutathione (GSH) and sperm quality.

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Section: Discussionmentioning

confidence: 86%

Therapeutic Effects of Exosome Therapy and Photobiomodulation Therapy on the Spermatogenesis Arrest in Male Mice After Scrotum Hyperthermia

Tabatabaee,

Darabi,

Soltani

et al. 2024

J Lasers Med Sci

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show abstract

“…However, few studies have conducted assessments of human sperm function, such as acrosome reaction, hyperactivation, and fertilization ability. Recent studies evaluated the effect of PBM therapy on the improvement of spermatogenesis in hyperthermia-induced azoospermia mouse models and found that the spermatogenesis process is significantly improved by PBM therapy [ 51 , 52 ]. In addition, accumulated evidence from animal studies suggested that PBM therapy improved sperm capacitation and fertilizing ability, as well as reproductive performance [ 53 , 54 ].…”

Section: Introductionmentioning

confidence: 99%

Applications of laser technology in the manipulation of human spermatozoa

Xue,

Xiong,

Cheng

et al. 2023

Reprod Biol Endocrinol

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The application of laser technology in the field of assisted reproductive technology (ART) has experienced rapid growth over the past decades owing to revolutionary techniques such as intracytoplasmic sperm injection (ICSI), preimplantation genetic testing (PGT), and in vitro manipulation of gametes and embryos. For male gametes, in vitro manipulation techniques include spermatozoa selection, sorting, immobilization, and quality assessment. A number of studies have been conducted to investigate the application of different laser technologies in the manipulation of human spermatozoa. However, there is a lack of a unified understanding of laser application in the in vitro manipulation of sperm and safety considerations in ART and, subsequently, the inability to make clear and accurate decisions on the clinical value of these laser technologies. This review summarizes the advancements and improvements of laser technologies in the manipulation of human spermatozoa, such as photobiomodulation therapy, laser trap systems for sperm analysis and sorting, laser-assisted selection of immotile sperm and laser-assisted immobilization of sperm prior to ICSI. The safety of those technologies used in ART is also discussed. This review will provide helpful and comprehensive insight into the applications of laser technology in the manipulation of human spermatozoa.

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A Comprehensive Systematic Review of the Effects of Photobiomodulation Therapy in Different Light Wavelength Ranges (Blue, Green, Red, and Near-Infrared) on Sperm Cell Characteristics in Vitro and in Vivo

Moradi,

Ghaffari Novin,

Bayat

2024

Reprod. Sci.

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Photobiomodulation therapy reverses spermatogenesis arrest in hyperthermia-induced azoospermia mouse model

Cited by 6 publications

References 29 publications

Therapeutic Effects of Exosome Therapy and Photobiomodulation Therapy on the Spermatogenesis Arrest in Male Mice After Scrotum Hyperthermia

Therapeutic Effects of Exosome Therapy and Photobiomodulation Therapy on the Spermatogenesis Arrest in Male Mice After Scrotum Hyperthermia

Applications of laser technology in the manipulation of human spermatozoa

A Comprehensive Systematic Review of the Effects of Photobiomodulation Therapy in Different Light Wavelength Ranges (Blue, Green, Red, and Near-Infrared) on Sperm Cell Characteristics in Vitro and in Vivo

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