Photobiomodulation Therapy Promotes Expansion of Epithelial Colony Forming Units

Abstract

Objective: This preliminary study examines the effects of low-dose light therapy, also called Photobiomodulation (PBM) therapy, on epithelial colony forming units (eCFUs) in epithelial cells from skin and mucosa to assess their potential to contribute to tissue regeneration. Also, preliminary comparison of basic PBM parameters such as wavelengths, light sources, and dose were evaluated in promoting eCFUs. Background Data: Regenerative medicine is at the brink of exploiting the tremendous potential offered by advances in stem cell biology. The two distinct aspects for utilization of stem cells, either resident (endogenous) or transplanted (exogenous), rely on cells amenable to expansion and being directed toward mature, functional tissues. Despite major progress in fundamental understanding of stem cell pluripotency, there remain fundamental challenges in applying these insights into clinical practice. Methods: PBM treatments with various devices, wavelengths, and doses were used on two epithelial cell lines and colony forming assays were performed. Results: This study noted a dose-dependent effect of 810 nm laser on increasing eCFUs, either in terms of size or numbers. Comparisons of different wavelengths and light sources noted better efficacy of collimated and coherent lasers compared to LEDs and broad-band light. Conclusions: PBM therapy promotes expansion of eCFUs that represent progenitors and stem cell populations capable of contributing to tissue repair and regeneration. Further exploration of the precise mechanisms would allow optimization of PBM clinical protocols to harness the regenerative potential of stem cells for wound healing and other clinical regenerative applications.

Photobiomodulation of freshly isolated human adipose tissue-derived stromal vascular fraction cells by pulsed light-emitting diodes for direct clinical application

“…Although the number of colonies indicated equal numbers of stem cells, the increased size of the colonies represents a higher proliferation potential after single red LLLT. This is exactly opposite to the study of Khan and Arany () where the size of the colonies was not affected by different red light sources but the number of colonies was significantly elevated. In this study, however, a 810‐nm laser at fluences of 1–3 J/cm 2 was used.…”

Accelerated burn wound healing with photobiomodulation therapy involves activation of endogenous latent TGF-β1

“…TGF-βs have been shown to promote proliferation and migration in a broad range of cell types from discrete anatomical sites 43 – 45 . We recently investigated the ability of the near-infrared laser to induce proliferation and enhance colony-forming units in epithelial keratinocytes 46 , 47 . These responses may contribute to the improved burn wound healing observed in this study.…”

A Fotobiomodulação No Processo Cicatricial Da Pele - Revisão Da Literatura / Photobiomodulation in Wound Healing Process - Literature Review

“…Os efeitos da fotobiomodulação se dão pela absorção da luz pelo fotorreceptor citocromo C oxidase, uma enzima da cadeia mitocondrial que quando excitada, ativa uma cascata de sinalização celular e acelera a transferência de elétrons; posteriormente, ocorre aumento eletroquímico na atividade mitocondrial, conferindo ainda aumento na síntese de ATP que levarão no processo cicatricial da pele à analgesia, ação anti-inflamatória, proliferação celular, neovascularização e regeneração tecidual [36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51] .…”