Photobiomodulation therapy modulates epigenetic events and NF-κB expression in oral epithelial wound healing

Gabriel, Amanda de Farias; Wagner, Vivian Petersen; Correa, Cintia; Webber, Liana Preto; Pilar, Emily Ferreira Salles; Curra, Marina; Carrard, Vinícius Coelho; Martins, Marco Antônio Trevizani; Martins, Manoela Domingues

doi:10.1007/s10103-019-02745-0

Cited by 40 publications

(29 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Conversely, PBM reportedly may reduce NFκB activation and subsequently reduce the expression of pro-inflammatory mediators in several diseases (111). Interestingly, de Farias Gabriel also reported that application of 660nm (4J/cm²) modulated NFκB activation leading to keratinocyte migration, resulting in improved wound healing in a rat model for oral epithelial wound healing (112). Hence, modulation of NFκB not only affects pathways related to inflammation but also those influencing wound healing.…”

Section: Nuclear Factor Kappa-light-chain-enhancer Of Activated B Celmentioning

confidence: 99%

Under the spotlight: mechanisms of photobiomodulation concentrating on blue and green light

Serrage

Heiskanen

Palin

et al. 2019

Photochem Photobiol Sci

100

View full text Add to dashboard Cite

show abstract

Section: Nuclear Factor Kappa-light-chain-enhancer Of Activated B Celmentioning

confidence: 99%

Under the spotlight: mechanisms of photobiomodulation concentrating on blue and green light

Serrage

Heiskanen

Palin

et al. 2019

Photochem Photobiol Sci

100

View full text Add to dashboard Cite

show abstract

“…The inflammatory cytokines further activate NF-κB through positive feedback and produce a cascade reaction, which continuously exacerbates inflammatory injury. The activation of NF-κB plays a key role in the initiation and process of mucosal inflammation, affecting injury and repair of oral mucosa [ 45 , 56 , 57 ]. The results showed that QWS downregulated the expressions of TLR4, TRAF6, MyD88, p-IκBα and NF-κB p65 protein in oral mucosal tissues of OU subjected to stomach heat syndrome in db/db mice and downregulated the ratio of p-IκBα/IκBα, suggesting inhibited efficacy on TLR4/MyD88/NF-κB pathway, which contributes to compromise of inflammatory injury and eventually improvement of OU.…”

Section: Discussionmentioning

confidence: 99%

Qingwei San treats oral ulcer subjected to stomach heat syndrome in db/db mice by targeting TLR4/MyD88/NF-κB pathway

Shi

Cheng

et al. 2022

Chin Med

View full text Add to dashboard Cite

Background Qingwei San (QWS), one of classic Chinese Medicine prescripts, has been widely used to treat stomach heat syndrome which manifests oral ulcer (OU), periodontitis and upper gastrointestinal bleeding for seven hundred years. However, the therapeutic effects of QWS on diabetic OU subjected to stomach heat syndrome are still ambiguous. In the study, we investigated the pharmacological mechanisms. Methods The main components of QWS aqueous extract were analyzed by LC–MS, and potential pathways of QWS targeting OU were predicted by network pharmacology. The db/db mice were administered with the decoction of dried Zingiber officinale Rosc. rhizome combined with NaOH cauterization to establish the model of diabetic OU subjected to stomach heat syndrome. Subsequently, the model mice were treated with QWS, and OU wound healing status were recorded. The pathological changes of gastric tissue and oral mucosa were evaluated using hematoxylin–eosin staining, and the morphology of collagen fibers in oral mucosa was assessed by Masson staining. The levels of thromboxane B2 (TXB2), 6-Keto-prostaglandin F1α (6-keto-PGF1α), interleukin-1 β (IL-1β), IL-2, IL-6, tumor necrosis factor-α (TNF-α), β-endorphin (β-EP) and 5-Hydroxytryptamine (5-HT) were determined by ELISA assay. The protein expressions of Toll-like receptor 4 (TLR4), TNF receptor associated factor 6 (TRAF6), myeloid differentiation factor 88 (MyD88), inhibitor of NF-κB alpha (IκΒα), p-IκΒα and nuclear factor kappa-B (NF-κB) p65 were measured by Western Blotting. Results A total of 183 compounds in QWS were identified by LC–MS, and identified 79 bioactive compounds corresponded to 269 targets and 59 pathways. QWS high-dose treatment significantly reduced the level of TXB2 and the ratio of TXB2/6-keto-PGF1α. Meanwhile, it improved mucosal pathological morphology, and reduced the area of OU and local edema. Simultaneously, the levels of TNF-α, IL-1β, IL-6, IL-2 and 5-HT, and the expressions of TLR4, TRAF6, MyD88, p-IκΒα and NF-κB p65 were decreased. Conclusion QWS treatment facilitates the healing of OU, ameliorates pathological morphologies of gastric and oral mucosa and decreases the levels of pro-inflammatory cytokines in db/db mice subjected to stomach heat syndrome, whose mechanism may be associated with the inhibition of TLR4/MyD88/NF-κB signaling pathway to exert anti-inflammatory effects.

show abstract

“…In the context of the laser biostimulatory effect on the proliferative and secretory functions of cells, Gabriel found that PBM therapy on surgical oral ulcer in the dorsum of rat tongue, using 660 nm continuous wave diode laser with energy density of 4 J/cm 2 applied for 10 consecutive day upregulated expression of NF-κB in keratinocytes on day 3 and downregulated it on day 10 which resulted in increased proliferation of keratinocytes in the early stage and differentiation in the last stages of wound repair. (42) Remaining Wound Area (RWA)…”

Section: Discussionmentioning

confidence: 99%

Effect of GaAlAs 940nm Photobiomodulation on palatal wound healing after Free Gingival Graft Surgery: A split mouth randomized controlled clinical trial

Tehrani

Aslroosta

Vafaei

2022

Preprint

View full text Add to dashboard Cite

Background: The aim of this study was to evaluate the effects of photobiomodulation (PBM) on wound healing, pain, and discomfort at free gingival graft (FGG) donor sites.Methods: Sixteen patients in need of bilateral FGG were selected for this randomized, controlled, triple-blinded, and split mouth clinical trial. The FGG donor sites in test group were treated with LLLT GaAlAs 940nm, 5J/cm2 immediately after surgery and every other day within the following ten days. The control group received sham irradiation. Remaining Wound Area (RWA), Epithelialization and color match were evaluated on the day of surgery and 7, 14, 21, 28, and 60 days after surgery. A questionnaire was administered to measure pain and bleeding in the first ten days after surgery. Results: RWA was significantly smaller in the test than control group on the days 7 (P<0.001) and 14 (P=0.048) after the surgery. Bleeding was higher in the test group than in the control group on the day of surgery (P=0.046). Pain and discomfort at the palatal donor site, however, had no significant difference between laser and control group during 11 days after the surgery (P> 0.05), nor did the Color match scores on the 28th and 60th days after the surgery (P> 0.05). Conclusions: It can be concluded that PBM enhances FGG donor site wound healing one and two weeks after the surgery.Trial registration: IRCT2017092036203N2, registered 01.11.2017.

show abstract

Photobiomodulation therapy modulates epigenetic events and NF-κB expression in oral epithelial wound healing

Cited by 40 publications

References 39 publications

Under the spotlight: mechanisms of photobiomodulation concentrating on blue and green light

Under the spotlight: mechanisms of photobiomodulation concentrating on blue and green light

Qingwei San treats oral ulcer subjected to stomach heat syndrome in db/db mice by targeting TLR4/MyD88/NF-κB pathway

Effect of GaAlAs 940nm Photobiomodulation on palatal wound healing after Free Gingival Graft Surgery: A split mouth randomized controlled clinical trial

Contact Info

Product

Resources

About