Photobiomodulation Therapy in the Proliferation and Differentiation of Human Umbilical Cord Mesenchymal Stem Cells: An In Vitro Study

Abstract

Introduction: Since photobiomodulation therapy (PBMT) favors in vitro mesenchymal stem cell (MSC) preconditioning before MSC transplantation, increasing the proliferation of these cells without molecular injuries by conserving their characteristics, in the present in vitro study we analyzed the effect of PBMT on the proliferation and osteogenic differentiation of human umbilical cord mesenchymal stem cells (hUCMSCs). Methods: Irradiation with an InGaAIP Laser (660 nm, 10 mW, 2.5 J/cm 2 , 0.08 cm 2 spot size, and 10 s) was carried out. The cells were divided into four groups: CONTROL [cells grown in Dulbecco's Modified Eagle Medium (DMEM)], OSTEO (cells grown in an osteogenic medium); PBMT (cells grown in DMEM+PBMT), and OSTEO+PBMT (cells grown in an osteogenic medium plus PBMT). The cell proliferation curve was obtained over periods of 24, 48 and 72 hours using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Osteogenic differentiation was analyzed by the formation of calcium nodules over periods of 7, 14 and 21 days. Morphometric analysis was performed to quantify the total area of nodular calcification. Results: The highest cell proliferation and cell differentiation occurred in the OSTEO+PBMT group, followed by the PBMT, OSTEO and CONTROL groups respectively, at the observed times (P < 0.05). Conclusion:PBMT enhanced the osteogenic proliferation and the differentiation of hUCMSCs during the periods tested, without causing damage to the cells and preserving their specific characteristics, a fact that may represent an innovative pretreatment in the application of stem cells.

Effects of Lectins from Alpinia purpurata Inflorescence (ApuL) and Schinus terebinthifolia Leaf (SteLL) on Human Leukemic Cell Lines and Mesenchymal Stem Cells

“…The MSCs isolation method was conducted without enzymatic treatment, based on the spontaneous migration of tissue cells and their adhesion to plastic with an average duration of 21 days. Identification was performed by known characteristics of MSCs, such as ability to adhere to plastic, morphology (fibroblastoid aspect), and by immunophenotyping [43].…”

Mesenchymal Stem Cells Based Treatment in Dental Medicine: A Narrative Review

“…Some research shows that the cells prepared for transplantation can be additionally conditioned with the visible light treatment, thus obtaining better final proliferation and differentiation of the cells [125]. Photobiomodulation therapy (PBMT) is well researched and widely accepted method in regenerative medicine and dentistry.…”

Low-Level Laser Irradiation Promotes Proliferation and Differentiation on Apical Papilla Stem Cells

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