Photobiomodulation reduces abdominal adipose tissue inflammatory infiltrate of diet‐induced obese and hyperglycemic mice

Yoshimura, Tania Mateus; Sabino, Caetano P.; Ribeiro, Martha S.

doi:10.1002/jbio.201600088

Cited by 21 publications

(25 citation statements)

References 32 publications

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“…Increased visceral adiposity and inflammatory infiltrate in adipose tissue are associated in the development of metabolic and cardiovascular diseases , therefore, we assessed the effects of PBMT on HFD‐induced adiposity and inflammatory infiltrate in visceral fat depots (Figure ). HFD increased epididymal adipocyte area (A), and epididymal adipose tissue inflammatory infiltrate (B) and PBMT attenuated these HFD‐induced responses.…”

Section: Resultsmentioning

confidence: 99%

“…Strategies, such as physical exercise training, reduce leucocyte infiltration into adipose tissue and its polarization to a pro‐inflammatory phenotype , improving insulin resistance . Using the same methodology for assessing inflammatory infiltrate, Yoshimura et al observed that high‐fat fed mice treated with PBMT (843 nm, 18 J of total energy on the abdominal surface) showed almost five times lower inflammatory infiltrate compared with nonirradiated animals. Therefore, our data together with previous studies shows that PBMT is able to reduce obesity‐induced adipose tissue inflammatory infiltrate.…”

Section: Resultsmentioning

confidence: 99%

“…It has also been demonstrated that PBMT exerts anti‐inflammatory effects, particularly in previously inflamed cells. For instance, Yoshimura et al showed that HFD‐fed, Sham‐treated mice presented five greater areas with inflammatory cells in abdominal fat than PBMT‐treated mice. Pro‐inflammatory cytokines can activate JNK, and therefore, as PBMT reduces inflammation, this therapy can reduce JNK phosphorylation.…”

Section: Introductionmentioning

confidence: 99%

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Insulin resistance is improved in high‐fat fed mice by photobiomodulation therapy at 630 nm

Silva

Ferraresi

Almeida

et al. 2020

Journal of Biophotonics

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Photobiomodulation therapy (PBMT) in the infrared spectrum exerts positive effects on glucose metabolism, but the use of PBMT at the red spectrum has not been assessed. Male Swiss albino mice were divided into low‐fat control and high‐fat diet (HFD) for 12 weeks and were treated with red (630 nm) PBMT or no treatment (Sham) during weeks 9 to 12. PBMT was delivered at 31.19 J/cm2, 60 J total dose per day for 20 days. In HFD‐fed mice, PBMT improved glucose tolerance, insulin resistance and fasting hyperinsulinemia. PBMT also reduced adiposity and inflammatory infiltrate in adipose tissue. Phosphorylation of Akt in epididymal adipose tissue and rectus femoralis muscle was improved by PBMT. In epididymal fat PBMT reversed the reduced phosphorylation of AS160 and the reduced Glut4 content. In addition, PBMT reversed the alterations caused by HFD in rectus femoralis muscle on proteins involved in mitochondrial dynamics and β‐oxidation. In conclusion, PBMT at red spectrum improved insulin resistance and glucose metabolism in HFD‐fed mice.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Insulin resistance is improved in high‐fat fed mice by photobiomodulation therapy at 630 nm

Silva

Ferraresi

Almeida

et al. 2020

Journal of Biophotonics

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“…Yoshimura et al [87] looked at a mouse model of obesity and type 2 diabetes [87]. Four weeks old male adult C57BL/6 mice were fed a hypercaloric high-fat diet (40% calories derived from fat) for eight weeks to induce obesity and hyperglycemia.…”

Section: Effects Of Pbm On Inflammation In Animal Models Of Diseasementioning

confidence: 99%

Mechanisms and applications of the anti-inflammatory effects of photobiomodulation

2017

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Photobiomodulation (PBM) also known as low-level level laser therapy is the use of red and near-infrared light to stimulate healing, relieve pain, and reduce inflammation. The primary chromophores have been identified as cytochrome c oxidase in mitochondria, and calcium ion channels (possibly mediated by light absorption by opsins). Secondary effects of photon absorption include increases in ATP, a brief burst of reactive oxygen species, an increase in nitric oxide, and modulation of calcium levels. Tertiary effects include activation of a wide range of transcription factors leading to improved cell survival, increased proliferation and migration, and new protein synthesis. There is a pronounced biphasic dose response whereby low levels of light have stimulating effects, while high levels of light have inhibitory effects. It has been found that PBM can produce ROS in normal cells, but when used in oxidatively stressed cells or in animal models of disease, ROS levels are lowered. PBM is able to up-regulate anti-oxidant defenses and reduce oxidative stress. It was shown that PBM can activate NF-kB in normal quiescent cells, however in activated inflammatory cells, inflammatory markers were decreased. One of the most reproducible effects of PBM is an overall reduction in inflammation, which is particularly important for disorders of the joints, traumatic injuries, lung disorders, and in the brain. PBM has been shown to reduce markers of M1 phenotype in activated macrophages. Many reports have shown reductions in reactive nitrogen species and prostaglandins in various animal models. PBM can reduce inflammation in the brain, abdominal fat, wounds, lungs, spinal cord.

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“…However, one of the urgent problems of aesthetic surgery, which is to develop an effective and safe method of removing unwanted fat, remains unresolved. There are many contemporary wave‐based methods of fat cell lipolysis and liposuction, such as ultrasonic , radiofrequency and laser technologies. The wave method is associated with radiation in a certain range of wavelengths (frequencies), which is effectively absorbed by the tissue, that is, optical, microwave or ultrasonic radiation.…”

Section: Introductionmentioning

confidence: 99%

Skin and subcutaneous fat morphology alterations under the LED or laser treatment in rats in vivo

Yanina

Navolokin

Bucharskaya

et al. 2019

Journal of Biophotonics

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The main objective of this work is to quantify the impact of photodynamic/photothermal treatment by using visible LED and NIR laser irradiation through the skin of subcutaneous fat in vivo followed up by tissue sampling and histology. The optical method may provide reduction of regional or site‐specific accumulations of abdominal or subcutaneous adipose tissue precisely and least‐invasively by inducing cell apoptosis and controlled necrosis of fat tissue. As photodynamic/photothermal adipose tissue sensitizers Brilliant Green (BG) or Indocyanine Green (ICG) dyes were injected subcutaneously in rats. The CW LED device (625 nm) or CW diode laser (808 nm) were used as light sources, respectively. Biopsies of skin together with subcutaneous tissues were taken for histology. The combined action BG‐staining and LED‐irradiation (BG + LED) or ICG‐staining and NIR‐laser irradiation (ICG + NIR) causes pronounced signs of damage of adipose tissue characterized by a strong stretching, thinning, folding and undulating of cell membranes and appearance of necrotic areas. As a posttreatment after 14 days only connective tissue was observed at the site of necrotic areas. The data obtained are important for safe light treatment of site‐specific fat accumulations, including cellulite. This work provides a basis for the development of fat lipolysis technologies and to move them to clinical applications. Schematics of animal experiment.

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Photobiomodulation reduces abdominal adipose tissue inflammatory infiltrate of diet‐induced obese and hyperglycemic mice

Cited by 21 publications

References 32 publications

Insulin resistance is improved in high‐fat fed mice by photobiomodulation therapy at 630 nm

Insulin resistance is improved in high‐fat fed mice by photobiomodulation therapy at 630 nm

Mechanisms and applications of the anti-inflammatory effects of photobiomodulation

Skin and subcutaneous fat morphology alterations under the LED or laser treatment in rats in vivo

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